Immune complexes in sera from patients with rheumatoid vasculitis induce polymorphonuclear cell-mediated injury to endothelial cells

Breedveld, Ferdinand C.; Heurkens, A. H. M.; Lafeber, G. J. M.; Hinsbergh, Victor W.M. van; Cats, A.

doi:10.1016/0090-1229(88)90084-0

Cited by 31 publications

(17 citation statements)

References 26 publications

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“…Some studies have suggested this to be mediated by polymorphonuclear cells,29 30 whereas others have found specific antibodies to endothelial cells in patients with rheumatoid vasculitis and suggested that these antibodies may play a part in the pathogenesis of the disease.3' Whatever the mechanism, damage to the blood vessel is likely to lead to dysfunction of the endothelium. It is interesting to note the significant positive correlation between vWF and t-PA Ag levels at baseline in the patients with RA alone.…”

Section: Discussionmentioning

confidence: 99%

Abnormal plasma fibrinolysis in patients with rheumatoid arthritis and impaired endothelial fibrinolytic response in those complicated by vasculitis.

Lau

McLaren

Hanslip

et al. 1993

Annals of the Rheumatic Diseases

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Objectives-(a) To assess plasma fibrinolytic parameters in patients with rheumatoid arthritis (RA) and to determine whether there are differences between patients with RA alone and those with RA complicated by vasculitis. (b) To determine if patients with RA respond differently to venous occlusion compared with normal subjects and to assess whether such a response differs in patients with RA alone and those with rheumatoid vasculitis. (c) To determine the extent of vascular damage in patients with rheumatoid vasculitis and if this correlates with the levels of plasma fibrinolytic parameters.

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Section: Discussionmentioning

confidence: 99%

Abnormal plasma fibrinolysis in patients with rheumatoid arthritis and impaired endothelial fibrinolytic response in those complicated by vasculitis.

Lau

McLaren

Hanslip

et al. 1993

Annals of the Rheumatic Diseases

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“…This destruction is caused by circulating C1q immune complexes containing RF and autoantibodies that form deposits in vessel walls, where they trigger an inflammatory reaction, which leads to endothelial cell (EC) injury and activation [30,31]. In the present study, we found that serum C4 levels in RV patients showed a negative correlation with MIF levels.…”

Section: Discussionmentioning

confidence: 77%

Elevated serum levels of macrophage migration inhibitory factor and their significant correlation with rheumatoid vasculitis disease activity

et al. 2011

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Macrophage migration inhibitory factor (MIF) is recognized to be an important mediator in several inflammatory disorders, including rheumatoid arthritis (RA) and vasculitis. To evaluate the role of MIF in rheumatoid vasculitis (RV), we determined serum levels of MIF by enzyme-linked immunosorbent assay in RA patients with and without vasculitis and assessed their relationship to disease activity. Serum was obtained from 95 RA patients during active disease states [49 without vasculitis, 35 with extra-articular manifestations without histologically proven vasculitis, and 11 with histologically proven vasculitis] and from 22 healthy individuals. Vasculitis disease activity was assessed using the Birmingham Vasculitis Activity Score (BVAS). MIF levels were significantly higher in RA patients than in controls. Moreover, MIF levels were significantly higher in RA patients with vasculitis than in those without vasculitic complications. In all RA patients, a statistically significant positive correlation was observed between serum MIF levels and each of the following: serum levels of C-reactive protein, rheumatoid factor, and thrombomodulin; and the erythrocyte sedimentation rate. In the RV group, the elevation of MIF levels correlated with the BVAS. Our findings suggest that MIF may serve as an additional serologic inflammatory marker of disease activity in RV, and it may be implicated in the pathogenesis of RV.

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“…is thought lo be mediated mainly by immune eomplexes [7], autoantibodies directed against EC have also been implicated in this process [6.8 12]. The observation that anti-endothelial cell antibodies (AEA) are found more often in RV patients than in RA supports an involvement of AL^A [5,1.^1, Thc presence of AEA in sera of these groups of autoimmune patients may thus be a marker for vasculitis or vascular damage.…”

Section: Introductionmentioning

confidence: 99%

Anti-endothelial cell antibodies in sera of patients with autoimmune diseases: comparison between ELISA and FACS analysis

Westphal

Boerbooms

Schalwijk

et al. 1994

Clinical and Experimental Immunology

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SUMMARYIn some patients suffering from rheumatoid arthritis (RA), vasculitis is a clear clinical manifestation, mentioned as rheumatoid vasculitis (RV). Autoantibodies directed againsi endothelial cells (AEA) have been implicated in the pathogenesis of this disorder, and it has been suggested in a number of studies that testing for AEA should be included in diagnosing RV. To test this hypothesis, we have evaluated the presence of AEA in sera of patients suffering from various autoimmune diseases, employing an ELISA with fixed cultured endothelial cells (EC). In ail the groups of patients ELlSA-positivc sera were present. A significant diflerence in percentage of positivity was found between the RA and RV group {P < 005). In addition, our results indicated that nol only antibodies directed against antigens on the EC membrane were detected, but atso antibodies directed against intracellular components like DNA. histones and cytoskeletal components. Therefore, we also tested all these patient sera on unfixed intact EC using indirect immunofluorescence followed by FACS analysis. Whereas in the total patient population 34 out of 65 patients were AEA-positivc as determined in the ELISA, only seven patienis were weakly positive when examined by flow cytomelry. We conclude that: (I) an ELISA on fixed EC does not specificaHy detect AEA, A positive test result is. however, to some extent correlated with the occurrence of vasculitis. and tnay therefore be helpful in diagnosing this disease: (ii) EACS analysis is a more suitable method than ELISA to measure the presence of membrane-specific AEA in patient sera; (iii) speciftc IgG-AEA are less common in patients suffering from autoimmune disorders than was assumed previously.

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Immune complexes in sera from patients with rheumatoid vasculitis induce polymorphonuclear cell-mediated injury to endothelial cells

Cited by 31 publications

References 26 publications

Abnormal plasma fibrinolysis in patients with rheumatoid arthritis and impaired endothelial fibrinolytic response in those complicated by vasculitis.

Abnormal plasma fibrinolysis in patients with rheumatoid arthritis and impaired endothelial fibrinolytic response in those complicated by vasculitis.

Elevated serum levels of macrophage migration inhibitory factor and their significant correlation with rheumatoid vasculitis disease activity

Anti-endothelial cell antibodies in sera of patients with autoimmune diseases: comparison between ELISA and FACS analysis

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