Immune-enhancing activity of polysaccharides from Hibiscus sabdariffa Linn. via MAPK and NF-kB signaling pathways in RAW264.7 cells

Shen, Chunyan; Zhang, Wenli; Jiang, Jian-Guo

doi:10.1016/j.jff.2017.03.060

Cited by 133 publications

(72 citation statements)

References 48 publications

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“…The untreated cells were used as control, LPS (40 lg mL À1 ) was used as the positive control. Subsequently, the supernatants of cells were collected and the levels of tumour necrosis factor-a (TNF-a) and interleukin-6 (IL-6) were measured using an ELISA kit (BD Biosciences, Mississauga, ON, Canada) according to the manufacturer's instructions; the level of nitric oxide (NO) was measured using the Griess reagent (Ren et al, 2017;Shen et al, 2017a). Samples were measured in triplicate, and this experiment was repeated three separate times.…”

Section: Cell Viabilitymentioning

confidence: 99%

Comparison of structural, antioxidant and immuno‐stimulating activities of polysaccharides from Tremella fuciformis in two different regions of China

Pan

Sun

Wang

et al. 2018

Int J of Food Sci Tech

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Summary In this study, we compared the chemical structure, antioxidant and immuno‐stimulating activities of two polysaccharides, TPS–FJ and TPS–SC, extracted from Tremella fuciformis from two of the major cultivation regions of China. TPS–FJ and TPS–SC contained protein and high uronic acid. Average molecular weights of TPS–FJ and TPS–SC were 45 461 and 25 981 kDa, respectively. A triple‐helix conformation was exhibited by TPS–FJ but not by TPS–SC. TPS–FJ and TPS–SC contained same monosaccharides but in different ratios. Both the polysaccharides displayed characteristic polysaccharide bands in their Fourier transform infrared and NMR spectra, shown high water and oil holding capacity, and retained antioxidant potential after simulated gastrointestinal digestion in vitro. TPS–FJ significantly (P < 0.05) stimulated the nitric oxide, tumour necrosis factor‐α and interleukin‐6 production of RAW 264.7 cells with higher reactions than those of TPS–SC in vitro. Thus, Tremella fuciformis polysaccharide can be used as a potential antioxidant and immunomodulatory ingredient in food industry.

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Section: Cell Viabilitymentioning

confidence: 99%

Comparison of structural, antioxidant and immuno‐stimulating activities of polysaccharides from Tremella fuciformis in two different regions of China

Pan

Sun

Wang

et al. 2018

Int J of Food Sci Tech

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“…The polysaccharide with higher content of uronic acid exhibited stronger immunostimulatory activity. In addition, low or moderate molecular‐weight polysaccharides were reported to exhibit stronger immunostimulatory activities than high molecular‐weight polysaccharides (Shen, Zhang, & Jiang, ). Therefore, these results suggested that the immunomodulatory activity DFHRP‐1 and DFHRP‐2 were influenced by several factors, and chemical degradation had capacity to strengthen the immunostimulatory activity of native polysaccharides.…”

Section: Resultsmentioning

confidence: 99%

Characterization, functional and biological properties of degraded polysaccharides from Hylocereus undatu s flowers

Zhao

Huang

2019

J Food Process Preserv

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The present study aimed at investigating the chemical degradation of the polysaccharide (designated as FHRP) isolated from the flowers of Hylocereus undatus (Haw.) Britton et Rose. Two degraded polysaccharides, designated as DFHRP‐1 and DFHRR‐2, were obtained from FHRP by using the Fe2+‐H2O2 degradation method. Physicochemical characterization showed that Fe2+‐H2O2 degradation could effectively decrease the molecular weight of FHRP, but did not significantly change chemical composition and monosaccharide composition. The functional assays indicated that DFHRP‐1 and DFHRR‐2 showed better oil‐holding, but weaker water‐holding and emulsifying capacities than FHRP. The results of bioactivity assays indicated that DFHRP‐1 and DFHRR‐2 exhibited better antioxidant, hypoglycemic, and immunostimulatory activities than FHRP. The functional and biological properties of the degraded polysaccharides from H. undatus flowers were greatly determined by its molecular weight. Overall, DFHRP‐1 and DFHRP‐2 might have potential applications as multi‐functional additives in foods, pharmaceuticals, and cosmetics. Practical applications It has been reported that degraded polysaccharides exhibited better water solubility, functional, and biological properties than native polysaccharide. In the present study, the degraded polysaccharides were obtained from the flowers of H. undatus by using the Fe2+‐H2O2 degradation method to modify its functional and biological properties. The results will provide valuable information for application of the degraded polysaccharides in the food industry.

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“…Recent studies revealed that polysaccharides with relatively low molecular weight have higher biological activities. [37][38][39] Moreover, numerous studies have demonstrated that content of glucose plays a key role in the improvement of hyperlipidemia. [17][18][19] Owing to the large amount of IPCM-2 and EPCM-2 in IPCM-1 and EPCM-1, the bioactivity of IPCM-1 and EPCM-1 were mainly caused by IPCM-2 and EPCM-2.…”

Section: Spatial Conformation Studiesmentioning

confidence: 99%

Structural characterization of polysaccharides fromCordyceps militarisand their hypolipidemic effects in high fat diet fed mice

et al. 2018

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Immune-enhancing activity of polysaccharides from Hibiscus sabdariffa Linn. via MAPK and NF-kB signaling pathways in RAW264.7 cells

Cited by 133 publications

References 48 publications

Comparison of structural, antioxidant and immuno‐stimulating activities of polysaccharides from Tremella fuciformis in two different regions of China

Comparison of structural, antioxidant and immuno‐stimulating activities of polysaccharides from Tremella fuciformis in two different regions of China

Characterization, functional and biological properties of degraded polysaccharides from Hylocereus undatu s flowers

Structural characterization of polysaccharides fromCordyceps militarisand their hypolipidemic effects in high fat diet fed mice

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