Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice

Abstract

Avian influenza viruses can be efficiently transmitted through mucous membranes, and conventional vaccines are not effective in protecting against mucosal infection by influenza viruses. To induce multiple immune responses in an organism, we constructed a recombinant Lactobacillus plantarum expressing the influenza virus antigen HA1 with the adjuvant dendritic cell-targeting peptide (DCpep). The recombinant L. plantarum strains NC8Δ-pWCF-HA1 and NC8Δ-pWCF-HA1-DCpep were used to immunize mice via oral administration, and the humoral, cellular and mucosal immune responses were evaluated. In addition, the serum levels of specific antibodies and hemagglutination inhibition (HI) levels were also measured. Our results showed that recombinant L. plantarum activated dendritic cells in Peyer’s patches (PPs), increased the numbers of CD4+IFN-γ+ and CD8+IFN-γ+ cells in the spleen and mesenteric lymph nodes (MLNs), and affected the ability of CD4+ and CD8+ cells to proliferate in the spleen and MLNs. Additionally, recombinant L. plantarum increased the number of B220+IgA+ cells in PPs and the level of IgA in the lungs and different intestinal segments. In addition, specific IgG, IgG1 and IgG2a antibodies were induced at high levels in the mice serum, specific IgA antibodies were induced at high levels in the mice feces, and HI potency was significantly increased. Thus, the recombinant L. plantarum strains NC8Δ-pWCF-HA1 and NC8Δ-pWCF-HA1-DCpep have potential as vaccine candidates for avian influenza virus.

New insights into natural products that target the gut microbiota: Effects on the prevention and treatment of colorectal cancer

“…The effect is the same as, or even better than, ICIs ( Tanoue et al, 2019 ). Stimulation of NK cells with Lactobacillus plantarum (Lp) enhances IL‐22 production, which decreases damage to the intestinal epithelial barrier ( Suzuki, 2013 ) and delays tumour formation ( Niu et al, 2021 ). Butyrate enhances Treg function in a murine model ( Arpaia et al, 2013 ; Smith et al, 2013 ).…”

Oral or intranasal immunization with recombinant Lactobacillus plantarum displaying head domain of Swine Influenza A virus hemagglutinin protects mice from H1N1 virus

“…SIgA is an important component of mucosal immunity. It is the most abundant immunoglobulin isotype in human secretions [53] and effectively protects against influenza virus infections [54]. It has been reported to be more important than IgG in protecting the upper respiratory tract, specifically the nose and trachea [54,55], primarily by reducing viral attachment and preventing virus internalisation at the mucosal surfaces, thereby preventing infection [54,[56][57][58].…”

Oral or intranasal immunization with recombinant Lactobacillus plantarum displaying head domain of Swine Influenza A virus hemagglutinin protects mice from H1N1 virus

“…The in uenza virus, a respiratory pathogen, contributes to high rates of morbidity and mortality in humans globally [39]. Although antiviral therapy is available, vaccination is still the most effective strategy for the prevention and control of in uenza [39][40][41][42]. In addition, since in uenza viruses naturally infect the mucosa, it is highly desirable to develop vaccines that can induce mucosal as well as systemic immune responses [41].…”

“…It is the most abundant immunoglobulin isotype in human secretions [43] and effectively protects against in uenza virus infections [40]. It has been reported to be more important than IgG in protecting the upper respiratory tract, speci cally the nose and trachea [40,44], primarily by reducing viral attachment and preventing virus internalisation at the mucosal surfaces, thereby preventing infection [40,[45][46][47]. In this study, L. plantarum ZN-3 was used as a vehicle for the delivery of truncated H1N1 HA1 antigen.…”