Immune gene expression in developing honey bees (<i>Apis mellifera</i> L.) simultaneously exposed to imidacloprid and <i>Varroa destructor</i> in laboratory conditions

Tesovnik, Tanja; Zorc, Minja; Gregorc, Aleš; Rinehart, Timothy A.; Adamczyk, John J.; Narat, Mojca

doi:10.1080/00218839.2019.1634463

Cited by 19 publications

(15 citation statements)

References 57 publications

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“…Recently, a significant increase of mite infestation in colonies adjacent to fields exposed to neonicotinoid treatments was reported 13,14 , suggesting a possible link between these pesticides and mite population abundance 15 . A synergistic negative effect of the association between Varroa and neonicotinoid insecticides on honey bees has also been reported [16][17][18][19] . This interaction has been largely overlooked so far, in spite of its potential remarkable importance, since any factor exacerbating the effects of mite infestation can have dramatic consequences on the survival of honey bee colonies 20 .…”

mentioning

confidence: 78%

Neonicotinoid Clothianidin reduces honey bee immune response and contributes to Varroa mite proliferation

et al. 2020

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The neonicotinoid Clothianidin has a negative impact on NF-κB signaling and on immune responses controlled by this transcription factor, which can boost the proliferation of honey bee parasites and pathogens. This effect has been well documented for the replication of deformed wing virus (DWV) induced by Clothianidin in honey bees bearing an asymptomatic infection. Here, we conduct infestation experiments of treated bees to show that the immune-suppression exerted by Clothianidin is associated with an enhanced fertility of the parasitic mite Varroa destructor, as a possible consequence of a higher feeding efficiency. A conceptual model is proposed to describe the synergistic interactions among different stress agents acting on honey bees.

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mentioning

confidence: 78%

Neonicotinoid Clothianidin reduces honey bee immune response and contributes to Varroa mite proliferation

et al. 2020

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“…In bees infected with Varroa mites, TMX altered the gene expression of their immune systems [ 71 ]. Similar changes in immune genes were observed in bees exposed to imidacloprid and infected with Varroa where the potent effect is variation over the three developmental stages (white-eyed pupae, brown-eyed pupae, and newly emerged bees) [ 72 ]. The exposure to imidacloprid decreased AMPs in the hemolymph and encapsulation response, owing to the reduction in hemocytes and prophenoloxidase [ 73 ].…”

Section: Main Causes Of Honeybee Colony Lossesmentioning

confidence: 66%

Bee Stressors from an Immunological Perspective and Strategies to Improve Bee Health

El-Seedi

Ahmed

El-Wahed

et al. 2022

Veterinary Sciences

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Honeybees are the most prevalent insect pollinator species; they pollinate a wide range of crops. Colony collapse disorder (CCD), which is caused by a variety of biotic and abiotic factors, incurs high economic/ecological loss. Despite extensive research to identify and study the various ecological stressors such as microbial infections, exposure to pesticides, loss of habitat, and improper beekeeping practices that are claimed to cause these declines, the deep understanding of the observed losses of these important insects is still missing. Honeybees have an innate immune system, which includes physical barriers and cellular and humeral responses to defend against pathogens and parasites. Exposure to various stressors may affect this system and the health of individual bees and colonies. This review summarizes and discusses the composition of the honeybee immune system and the consequences of exposure to stressors, individually or in combinations, on honeybee immune competence. In addition, we discuss the relationship between bee nutrition and immunity. Nutrition and phytochemicals were highlighted as the factors with a high impact on honeybee immunity.

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“…Molecular effects thus need to be verified. Using the qPCR approach, Tesovnik et al (2019) examined honey bee adult immune response-related genes after exposure to 20 ppb imidacloprid during the larval stage for 4 continuous d. They identified the downregulation of immune responses, including Toll, JAK/STAT, IMD, JNK, and antimicrobial peptides, in both white-eyed and brown-eyed pupae, while those of adults were upregulated [ 67 ] ( Table 2 c).…”

Section: The Effects Of Sublethal Dosages Of Imidacloprid Exposure During the Larval Stage From A Molecular Perspectivementioning

confidence: 99%

“…After exposure to 500 ppb imidacloprid during the larval stage, developed adults showed a substantial impact on the expression of detoxification, metabolism, neuron development, structural protein, and transporters and receptors [ 66 ]. While providing 20 ppb imidacloprid during the larval stage, immune pathway-related genes were significantly downregulated in pupae and newly emerged adults [ 67 ]. From the perspective of the developmental queue, the affected genes and functional pathways were diverse among workers of different ages.…”

Section: The Affected Molecular Pathways At Different Ages Of Honey Bee Workersmentioning

confidence: 99%

Chronic Effects of Imidacloprid on Honey Bee Worker Development—Molecular Pathway Perspectives

Chen

Tzeng

Yang

2021

IJMS

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Sublethal dosages of imidacloprid cause long-term destructive effects on honey bees at the individual and colony levels. In this review, the molecular effects of sublethal imidacloprid were integrated and reported. Several general effects have been observed among different reports using different approaches. Quantitative PCR approaches revealed that imidacloprid treatments during the adult stage are expressed as changes in immuneresponse, detoxification, and oxidation-reduction response in both workers and queens. In addition, transcriptomic approaches suggested that phototransduction, behavior, and somatic muscle development also were affected. Although worker larvae show a higher tolerance to imidacloprid than adults, molecular evidence reveals its potential impacts. Sublethal imidacloprid treatment during the larval stage causes gene expression changes in larvae, pupae, and adults. Transcriptome profiles suggest that the population and functions of affected differentially expressed genes, DEGs, vary among different worker ages. Furthermore, an early transcriptomic switch from nurse bees to foragers was observed, suggesting that precocious foraging activity may occur. This report comprehensively describes the molecular effects of sublethal dosages of imidacloprid on the honey bee Apis mellifera. The corresponding molecular pathways for physiological and neurological responses in imidacloprid-exposed honey bees were validated. Transcriptomic evidence suggests a global and sustained sublethal impact of imidacloprid on honey bee development.

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Immune gene expression in developing honey bees (Apis mellifera L.) simultaneously exposed to imidacloprid and Varroa destructor in laboratory conditions

Cited by 19 publications

References 57 publications

Neonicotinoid Clothianidin reduces honey bee immune response and contributes to Varroa mite proliferation

Neonicotinoid Clothianidin reduces honey bee immune response and contributes to Varroa mite proliferation

Bee Stressors from an Immunological Perspective and Strategies to Improve Bee Health

Chronic Effects of Imidacloprid on Honey Bee Worker Development—Molecular Pathway Perspectives

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