Immune infiltration and a ferroptosis-associated gene signature for predicting the prognosis of patients with endometrial cancer

Yin, Wenwu; Liao, Fuchun; Chen, Mengjie; Qin, Xiaoqing; Lai, Hao; Lin, Yuan; Yao, Desheng

doi:10.18632/aging.203190

Cited by 47 publications

(29 citation statements)

References 59 publications

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“…The FRGs containing 275 protein coding genes were downloaded from FerrDb and GeneCards (https://www.genecards.org/). 17 The details of FRGs were listed in Supplementary Table 1.…”

Section: Gse Datasets and Ferroptosis-related Genes (Frgs) Acquisitionmentioning

confidence: 99%

Identification of ferroptosis-related molecular markers in glomeruli and tubulointerstitium of lupus nephritis

Wang

Lin

Feng

et al. 2022

Lupus

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Purpose Ferroptosis, characterized by iron accumulation and lipid peroxidation, is a newly demonstrated form of programed cell death. Present studies reveal that ferroptosis is involved in tumor and neurodegenerative disease. Regarding its roles in the development of LN, it is least interrogated. In this study, we explored whether ferroptosis is activated and how does it change at transcriptomic level in LN. Methods 4-Hydroxynonenal (4-HNE) was stained to explore whether ferroptosis is activated. Subsequently, by using bioinformatic methods, public GSE32591 dataset was analyzed. Ferroptosis-related differentially expressed genes (FR-DEGs) were identified in both glomeruli and tubulointerstitium. Immune cell infiltration was evaluated. Correlation between FR-DEGs and infiltrated immune cells was also calculated. Finally, dataset of GSE113342, qPCR, and immunofluorescence staining were also used or performed to validate the results. Results Expression of 4-HNE was significantly increased in both glomeruli and tubulointerstitium. At transcriptomic level, 19 FR-DEGs in glomeruli and 15 FR-DEGs in tubulointerstitium including genes of iron metabolism, antioxidant system inhibitors, and ferroptosis suppressors were significantly altered in LN. Of which, LTF, CYBB, and CCL5 were upregulated and G0S2 and AKR1C1 were downregulated in both glomeruli and tubulointerstitium of LN. qPCR further validated the alteration of LTF, CYBB, CCL5, G0S2, and AKR1C1 in the whole kidney. Correlation analysis showed that CYBB positively correlated with monocyte infiltration in glomeruli and positively correlated with response to therapy. Conclusion Lipid peroxidation was aberrantly activated in LN, suggesting the activation of ferroptosis. LTF, CYBB, CCL5, G0S2, and AKR1C1, especially CYBB, might be good biomarkers of ferroptosis in LN.

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“…The FRGs containing 275 protein coding genes were downloaded from FerrDb and GeneCards (https://www.genecards.org/). 17 The details of FRGs were listed in Supplementary Table 1.…”

Section: Gse Datasets and Ferroptosis-related Genes (Frgs) Acquisitionmentioning

confidence: 99%

Identification of ferroptosis-related molecular markers in glomeruli and tubulointerstitium of lupus nephritis

Wang

Lin

Feng

et al. 2022

Lupus

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“…In addition, cytoplasm FANCD2 was discovered to be capable of binding protein participating in the native immune system, cell reaction to thermal stress, amyloid fiber formation, and estrogen-mediated signal transduction [ 27 ]. High ATP5MC3 expression predicted a poor prognosis in prostate cancer, colon cancer, and endometrial cancer [ 28 – 30 ]. TGF- β 1-mediated inhibition of SLC7A11 drives vulnerability to GPX4 suppression in hepatic cell cancer [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

A Comprehensive Prognostic and Immune Analysis of Ferroptosis-Related Genes Identifies SLC7A11 as a Novel Prognostic Biomarker in Lung Adenocarcinoma

Wang

et al. 2022

Journal of Immunology Research

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Lung adenocarcinoma (LUAD) is still one of the illnesses with the greatest mortality and morbidity. As a recently identified mode of cellular death, the activation of ferroptosis may promote the effectiveness of antitumor therapies in several types of tumors. However, the expression and clinical significance of Ferroptosis-associated genes in LUAD are still elusive. The RNA sequencing data of LUAD and relevant clinical data were downloaded from The Cancer Genome Atlas (TCGA) datasets. Subsequently, potential prognostic biomarkers were determined by the use of biological information technology. The R software package “ggalluvial” was applied to structure Sanguini diagram. Herein, our team screened 14 dysregulated ferroptosis-associated genes in LUAD. Among them, only four genes were associated with clinical outcome of LUAD patients, including ATP5MC3, FANCD2, GLS2, and SLC7A11. In addition, we found that high SLC7A11 expression predicted an advanced clinical progression in LUAD patients. Additionally, 8 immune checkpoint genes and 7 immune cells for LUAD were recognized to be related to the expression of SLC7A11. KEGG assays indicated that high expression of SLC7A11 might participate in the modulation of intestinal immune network for IgA generation and Staphylococcus aureus infection. Overall, our findings revealed that SLC7A11 might become a potentially diagnostic biomarker and SLC7A11 might serve as an independent prognosis indicator for LUAD.

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“…Although there have been some articles using genetic models to predict the prognosis of EC patients ( Wang H. et al, 2020 ; Zhang et al, 2020a ; Wang X. et al, 2021 ; Weijiao et al, 2021 ), there is no literature that systematically uses necroptosis-related miRNAs to predict the prognosis of EC patients. As far as we know, this study is the first to systematically use necroptosis-related miRNAs to predict the prognosis of EC patients.…”

Section: Discussionmentioning

confidence: 99%

Necroptosis-Related miRNA Biomarkers for Predicting Overall Survival Outcomes for Endometrial Cancer

Song

Sheng

et al. 2022

Front. Genet.

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Endometrial cancer (EC) is the gynecological tumor with the highest incidence. In recent years, it has been proved that necroptosis is a method of cell death related to EC. However, the expression of necroptosis-related miRNA in EC and its correlation with prognosis still ill-defined. Use the Cancer Genome Atlas (TCGA) cohort to obtain prognostic data and related clinical data for ECs and normal endometrium tissues. In this study, we identified three necroptotic regulatory miRNAs that are necroptosis-related and survival-related miRNAs (DENSMs) between normal endometrium tissues and EC from 13 necroptosis-related miRNAs. The three DENSMs signature was built to develop prognostic model and classified all EC patients into a high or low risk group. EC patients in the low-risk group showed significantly higher survival possibilities than those in the high-risk group (p = 0.0242), and the risk score was found to be an independent prognosis factor for predicting the OS of EC patients (p = 0.0254) in multivariate Cox regression. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed dephosphorylation, microtubule, protein serine/threonine kinase activity, PI3K-Akt signaling pathway and MAPK signaling pathway are closely related to it. In conclusion, the risk prediction model based on necroptosis-related miRNAs can effectively predict the prognosis of EC patients.

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Immune infiltration and a ferroptosis-associated gene signature for predicting the prognosis of patients with endometrial cancer

Cited by 47 publications

References 59 publications

Identification of ferroptosis-related molecular markers in glomeruli and tubulointerstitium of lupus nephritis

Identification of ferroptosis-related molecular markers in glomeruli and tubulointerstitium of lupus nephritis

A Comprehensive Prognostic and Immune Analysis of Ferroptosis-Related Genes Identifies SLC7A11 as a Novel Prognostic Biomarker in Lung Adenocarcinoma

Necroptosis-Related miRNA Biomarkers for Predicting Overall Survival Outcomes for Endometrial Cancer

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