Immune modulation of antibody response to porcine reproductive and respiratory syndrome virus glycoprotein 5 (GP5) using anti-idiotypic antibodies in mouse model

Abstract

Porcine reproductive and respiratory syndrome (PRRS), caused by the PRRS virus (PRRSV), is a disease of great economic importance for the swine industry worldwide. PRRSV is a member of the family Arteriviridae in the order Nidovirales. PRRSV is an enveloped positive single-stranded RNA virus, which encodes an approximately 4,000-aminoacid large replicase polyprotein (open reading frame [ORF] 1 a and 1 b) and six structural proteins of 130 to 265 amino acids (ORFs 2 to 7). Three of these structural proteins have been identified as envelope (25 kDa), matrix (19 kDa), and nucleocapsid (15 kDa) proteins, i.e. GP5, M, and N proteins, respectively. PRRSV infection poses a challenge to current serodiagnostic and vaccination strategies. A better understanding of the mechanisms that regulate immunity to this virus will aid in the development of more effective vaccines, as well as serodiagnostic CHAPTER 3. Swine Auto-anti-Idiotypic Antibody Specific for Antibodies Against GPS Antigen of Porcine Reproductive and Respiratory Syndrome Virus Elicits Specific