Immune modulation through RNA interference-mediated silencing of CD40 in dendritic cells

A lipid nanoparticle for the efficient delivery of siRNA to dendritic cells

“…In the case of a clinical application, however, a non-viral approach can be more desirable than a viral vector, which have been reported to show serious side effects in clinical trials [8]. Some studies have demonstrated that DCs can be gene silenced using non-viral methods including commercially available transfection reagents such as Lipofectamine TM 2000 [9][10][11][12][13][14]. The small interfering RNA (siRNA) doses used in these reported studies were 100 nM -4 μM in transfection to DCs, which was considerably higher than the dose used in transfection when other cell lines are used.…”

Antigen-Presenting Dendritic Cells Rescue CD4-Depleted CCR2−/−Mice from LethalHistoplasma capsulatumInfection

“…DC in lungs of infected CCR2 Ϫ/Ϫ mice exhibited a phenotype consistent with TH2 cytokine instruction. A decreased proportion of CD8 Ϫ cDC expressed CD40, a costimulatory molecule that restricts IL-4 production by CD4 ϩ T cells (22). In addition, DC from CCR2 Ϫ/Ϫ mice bear less MHCII than control DC (43).…”

“…Increased surface expression of the Notch ligand Jagged 1 on human DC (29) or of Jagged 2 on murine DC (48) and decreased expression of the maturation marker CD40 (22) promote a TH2 phenotype as well. In contrast, pathogens that induce production of IL-12 by DC promote TH1 polarization and simultaneously inhibit IL-4 production (30).…”

Development of a multifunctional envelope-type nano device and its application to nanomedicine

“…In the case of a clinical applications, however, a non-viral approach might well be more desirable. Gene silencing in DCs using non-viral vectors such as LF2000 have been reported, but the siRNA doses used in these reported studies were 100 nM-4 μM for transfection [57][58][59][60][61][62].…”