Immune profiles of T lymphocyte subsets in adipose tissue of obese mouse induced by high-fat diet

Zhang, Guojun; Wang, Xia; Feng, Huanhuan; Sun, Aiping; Sun, Shuming; Lu, Bin; Song, Xiangfeng

doi:10.18805/ijar.v0iof.9141

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…There were evidences that serum levels of the proinflammatory SAA were moderately increased in DIO rats (Uhlar and Whitehead, 1999;Dimitrov et al, 2015). Other studies reported alteration of immune profile of rodents in response of high fat diet (Guojun Zhang et al, 2017). Our experiments have shown no significant difference between the SAA levels in obese, compared with control animals.…”

Section: General Time Distribution Of Survival In Groupscontrasting

confidence: 58%

Influence of obesity on the tolerance of abdominal hypertension in rats-some mechanical aspects

Turiyski

Dimitrov

Ardasheva

et al. 2018

IJAR

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The present study investigates the relations between obesity and survival during intra-abdominal hypertension (IAH) in diet induced obesity Wistar rats. The animals from model group were fed with high-fat diet for 45 days. Intra-abdominal pressure in both groups (control and model) was elevated by manual system for air insufflations. The pressure was of value (25mmHg) corresponding to the typical values observed in abdominal compartment syndrome. Quantification of the serum levels of serum amyloid A was carried out after deflation. The results interpretation was made in correlation with clinical observations in patients with proved cardiovascular diseases caused by obesity, as well as with patients to whom surgery had caused an increased intra-abdominal pressure. Experimental observations have shown increased survival in diet induced obesity rats with IAH compared to control rats with IAH. Increased resistance to IAH is due to changes in the structure and in the mechanical characteristics of the abdominal wall, following obesity.

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Section: General Time Distribution Of Survival In Groupscontrasting

confidence: 58%

Influence of obesity on the tolerance of abdominal hypertension in rats-some mechanical aspects

Turiyski

Dimitrov

Ardasheva

et al. 2018

IJAR

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“…Obesity regulates immune cells in adipose tissue ( 22 ). Adipose tissue is a vital immune organ that stores fat and provides energy ( 23 ). Obesity changes the immune cell landscape in adipose tissues, causing the pro-inflammatory cells and CD8+T cells to gradually dominate ( 24 , 25 ).…”

Section: Discussionmentioning

confidence: 99%

Investigating the change in gene expression profile of blood mononuclear cells post-laparoscopic sleeve gastrectomy in Chinese obese patients

Liu

Chen²,

Ran³

et al. 2023

Front. Endocrinol.

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BackgroundLaparoscopic sleeve gastrectomy (LSG) is a sustainable technique that effectively treats morbid obesity. However, the molecular mechanisms underlying the improvement of metabolic health following this process warrants more investigation. This study investigates LSG-related molecules and uses bulk RNA-sequencing high-throughput analysis to unravel their regulatory mechanisms.MethodsPeripheral blood mononuclear cells (PBMC) were collected from ten obese patients with BMI ≥ 32.5 kg/m2 in the Department of General Surgery of Kunming First People’s Hospital. After LSG, patients were followed up for one month, and blood samples were retaken. Blood samples from ten patients before and after LSG and bulk RNA-Seq data were analyzed in this study. LSG-associated gene expression was detected by weighted gene coexpression network analysis (WGCNA) and differential analysis. Subsequently, essential signature genes were identified using logistic least absolute shrinkage and selection operator (LASSO) and support vector machine-recursive feature elimination (SVM-RFE) algorithms. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and single-sample gene set enrichment analysis (ssGSEA) were utilized to reveal the potential functions of the target genes. Furthermore, the Pearson correlation of signature genes with leptin and lipocalin was also explored. Finally, we constructed a robust endogenous RNA (ceRNA) network based on miRWalk and starBase databases.ResultsWe identified 18 overlapping genes from 91 hub genes, and 165 differentially expressed mRNAs (DE-mRNA), which were revealed to be significantly associated with immune cells, immune response, inflammatory response, lipid storage, and localization upon functional enrichment analysis. Three signature genes, IRF1, NFKBIA, and YRDC, were identified from the 18 overlapping genes by LASSO and SVM-REF algorithms. The logistic regression model based on the three signature genes highlighted how robustly they discriminated between samples. ssGSEA indicated these genes to be involved in lipid metabolism and degradation pathways. Moreover, leptin levels were significantly reduced in patients undergoing LSG, and NFKBIA significantly negatively correlated with leptin. Finally, we identified how the long non-coding RNA (lncRNA) ATP2B1-AS1 regulated the expression of the signature genes by competitively binding to six microRNAs (miRNAs), which were hsa-miR-6509-5p, hsa-miR-330-5P, hsa-miR-154-5P, hsa-miR-145-5P, hsa-miR4726-5P and hsa-miR-134-5P.ConclusionThis study identified three critical regulatory genes significantly differentiated between patients before and after LSG treatment and highlighted their potentially crucial role after bariatric surgery. This provides novel insights to increase our understanding of the underlying mechanisms of weight loss and associated metabolic improvement after bariatric surgery.

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Immune profiles of T lymphocyte subsets in adipose tissue of obese mouse induced by high-fat diet

Cited by 2 publications

References 19 publications

Influence of obesity on the tolerance of abdominal hypertension in rats-some mechanical aspects

Influence of obesity on the tolerance of abdominal hypertension in rats-some mechanical aspects

Investigating the change in gene expression profile of blood mononuclear cells post-laparoscopic sleeve gastrectomy in Chinese obese patients

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