Immune proteomics of <i>Staphylococcus aureus</i>

Bröker, Barbara M.; Belkum, Alex van

doi:10.1002/pmic.201100010

Cited by 30 publications

(20 citation statements)

References 93 publications

(140 reference statements)

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“…Although patients with infection had higher IgG titers than the control patients, the control patients also had significant IgG titers, some overlapping with the infected population. High IgG titers in noninfected individuals have been observed by others [5,8,13,17,[39][40][41][42][43] and may be related to the high rates of colonization with S aureus, especially among children. Most newborns first contact S aureus just after birth and the colonization rates of S aureus are high in children and adolescents (20%-24].…”

Section: A Predominant Pattern Of Antigens Recognized By Patients or mentioning

confidence: 96%

“…Most newborns first contact S aureus just after birth and the colonization rates of S aureus are high in children and adolescents (20%-24]. Even though the colonization rate decreases in adults, most humans are regularly exposed to S aureus possibly with repeated subclinical infections [5]. Moreover, some patients are persistent carriers of S aureus and have higher antibody levels than noncarriers [41,42].…”

Section: A Predominant Pattern Of Antigens Recognized By Patients or mentioning

confidence: 99%

See 1 more Smart Citation

A Diagnostic Serum Antibody Test for Patients With Staphylococcus aureus Osteomyelitis

Nishitani

Beck

Rosenberg

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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Background Because immunity against Staphylococcus aureus has not been fully elucidated, there is no diagnostic test to gauge how robust a patient's host response is likely to be. Therefore, we aimed to develop a test for specific antibodies in serum with diagnostic and prognostic potential. Questions/Purposes We describe the development and validation of a multiplex immunoassay for characterizing a patient's immune response against 14 known S aureus antigens, which we then used to answer four questions: (1) Do certain antigens predominate in the immune response against S aureus? (2) Is there a predominant pattern of antigens recognized by patients and mice with infections? (3) Is the immunoglobulin G (IgG) response to any single antigen a useful predictor of ongoing S aureus infection? (4) Does measurement of the combined response against all 14 antigens provide a better predictor of ongoing infection? Methods A case-control study was performed. Sera were collected from 35 consecutive patients with S aureus culture-confirmed (methicillin-sensitive S aureus or methicillin-resistant S aureus) musculoskeletal infections The institution of one or more of the authors (EMS, SLK, JLD, CAB) has received, during the study period, funding from the National Institutes of Health, NIAMS (Bethesda, MD, USA) and (EMS, SLK, JLD) the AOTrauma Clinical Priority Program (Davos, Switzerland). One of the authors certifies that he (JLD) or she or a member of his or her immediate family, has received or may receive payments or benefits, during the study period, an amount of USD 10,000 to 100,000 from Telephus Medical LLC (San Diego, CA, USA) and an amount of less than USD 10,000 from Calorics Pharmaceuticals (Waltham, MA, USA). One of the authors certifies that he (EMS) or she or a member of his or her immediate family, has received or may receive payments or benefits, during the study period, an amount of USD 10,000 to 100,000 from Telephus Medical LLC (San Diego, CA, USA). One of the authors certifies that he (SLK) or she or a member of his or her immediate family, has received or may receive payments or benefits, during the study period, an amount of less than USD 10,000 from Surgical Excellence Healthcare Quality Consulting, and an amount of USD 10,000 to 100,000 from Sage Publications (Thousand Oaks, CA, USA) One of the authors certifies that he (CAB) or she or a member of his or her immediate family, has received or may receive payments or benefits, during the study period, an amount of USD 10,000 to 100,000 from the FDA (Silver Spring, MD, USA), and an amount of USD 10,000 to 100,000 from Boston Scientific (Marlborough, MA, USA), an amount of USD 10,000 to 100,000 from Lundbeck Inc (Deerfield, IL, USA), an amount of USD 10,000 to 100,000 from Patient-Centered Outcomes Research Institute (PCORI, Washington, DC, USA), and an amount of less than USD 10,000 from Auspex Pharmaceuticals (La Jolla, CA, USA). All ICMJE Conflict of Interest Forms for authors and Clinical Orthopaedics and Related Research 1 editors and board mem...

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Section: A Predominant Pattern Of Antigens Recognized By Patients or mentioning

confidence: 96%

Section: A Predominant Pattern Of Antigens Recognized By Patients or mentioning

confidence: 99%

A Diagnostic Serum Antibody Test for Patients With Staphylococcus aureus Osteomyelitis

Nishitani

Beck

Rosenberg

et al. 2015

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

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“…Several studies have characterized the S. aureus proteins that are targeted by the host antibody response following infection or colonization (302–304). This group of antigens is referred to as the immune proteome, and identifies proteins that are expressed in vivo and may be important to virulence.…”

Section: Evasion Of the Host Immune Systemmentioning

confidence: 99%

The Staphylococcal Biofilm: Adhesins, Regulation, and Host Response

2016

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The Staphylococci comprise a diverse genus of Gram-positive, non-motile commensal organisms that inhabit the skin and mucous membranes of humans and other mammals. In general, Staphylococci are benign members of the natural flora, but many species have the capacity to be opportunistic pathogens, mainly infecting individuals who have medical device implants or are otherwise immunocompromised. S. aureus and S. epidermidis are a major source of hospital-acquired infections and are the most common causes of surgical site infections and central line-associated bloodstream infections. The ability of Staphylococci to form biofilms in vivo makes them highly resistant to chemotherapeutics and leads to chronic diseases. These biofilm infections include osteomyelitis, endocarditis, medical device implants, and persistence in the cystic fibrosis lung. Here, we provide a comprehensive analysis of our current understanding of Staphylococcal biofilm formation, with an emphasis on adhesins and regulation, while also addressing how Staphylococcal biofilms interact with the immune system. On the whole, this review will provide a thorough picture of biofilm formation of the Staphylococcus genus and how this mode of growth impacts the host.

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“…Furthermore, anti-staphylococcal IgG and IgA levels and Sa antigenic diversity show great individual variability in healthy individuals and in the presence of bacteremia 17,18. This variability has been attributed to strain characteristics, with strain-specific arsenals of evasion mechanisms and differences in the host immune response to this bacterium 19.…”

Section: Discussionmentioning

confidence: 99%

Passive acquisition of anti-Staphylococcus aureus antibodies by newborns via transplacental transfer and breastfeeding, regardless of maternal colonization

Nadaf¹,

Lima²,

Stranieri³

et al. 2016

Clinics

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OBJECTIVE:To investigate the transmission of anti-Staphylococcus aureus (Sa) IgG, IgG1 and IgG2 via placental transfer and the transfer of IgA via the colostrum according to maternal Sa carrier status at delivery.METHODS:We evaluated anti-Sa IgG, IgG1 and IgG2 in maternal and cord sera and IgA in colostrum from a case (n=49, Sa+) and a control group (n=98, Sa-).RESULTS:Of the 250 parturients analyzed for this study, 49 were nasally colonized with S. aureus (prevalence of 19.6%). Ninety-eight non-colonized subjects were selected for the control group. The anti-Sa IgG, IgG1 and IgG2 levels and the IgG avidity indexes in the maternal and cord sera did not differ between the groups, with a low transfer ratio of anti-Sa IgG to the newborns in both groups. The anti-Sa IgG2 titers were significantly higher than the IgG1 titers in the maternal and cord sera. Inversely, the transfer ratios were higher for anti-Sa IgG1 compared with IgG2; however, no differences between the groups were detected. The Sa-specific IgA levels and avidity indexes in the colostrum were equivalent between groups.CONCLUSIONS:Maternal Sa nasal colonization at delivery is not associated with higher antibody levels in the mother or newborns. The high titers of anti-Sa IgG2 found in the cord serum indicate a greater reactivity with non-protein antigens, which may further contribute to the susceptibility to staphylococcal infections at birth. The presence of IgA in the colostrum with avidity to S. aureus reinforces the importance of breastfeeding shortly after birth.

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Immune proteomics of Staphylococcus aureus

Cited by 30 publications

References 93 publications

A Diagnostic Serum Antibody Test for Patients With Staphylococcus aureus Osteomyelitis

A Diagnostic Serum Antibody Test for Patients With Staphylococcus aureus Osteomyelitis

The Staphylococcal Biofilm: Adhesins, Regulation, and Host Response

Passive acquisition of anti-Staphylococcus aureus antibodies by newborns via transplacental transfer and breastfeeding, regardless of maternal colonization

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