Immune-pyroptosis-related genes predict the prognosis of kidney renal clear cell carcinoma

Zhang, Minhao; Liu, Y E; Gao, Yue; Zhao, Chenggui; Pan, Ke-Hao

doi:10.1016/j.tranon.2023.101693

Cited by 2 publications

(3 citation statements)

References 43 publications

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“…Several renal cancer cell lines (caki-1, caki-2, 786–0, A498) and normal renal cell lines (HK2,293 T) were used for subsequent experiments [ 12 , 13 ], obtained from the American Type Culture Collection (ATCC), USA. All cells were cultured at 37 °C and 5% CO 2 in the appropriate medium, the details was listed in Additional file 1 .…”

Section: Methodsmentioning

confidence: 99%

CCDC25 suppresses clear cell renal cell carcinoma progression by LATS1/YAP-mediated regulation of the hippo pathway

Tan,

Liu,

et al. 2024

Cancer Cell Int

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Background Clear cell renal cell carcinoma (ccRCC) is one of the most prevalent renal cancers, and the molecular mechanisms underlying its progression are still not fully understood. The expression of CCDC25, a notably underexpressed gene in many tumors, has been understudied in ccRCC. This research aims to explore the role of CCDC25 in ccRCC's clinical outcomes and uncover the molecular pathways influenced by it. Methods A multi-tiered approach was adopted involving bioinformatic analysis, tissue sample evaluation, in vitro and in vivo experiments. CCDC25 expression levels in tumor vs. normal tissues were quantified using Western blot and immunofluorescence studies. Cell proliferation and migration were analyzed using CCK8, EDU, Transwell assays, and wound healing assays. RNA sequencing was performed to elucidate the molecular pathways affected, followed by detailed protein–protein interaction studies and mouse xenograft models. Results CCDC25 was predominantly underexpressed in ccRCC tumors and associated with advanced clinical stages and poor prognosis. Overexpression of CCDC25 in renal cancer cell lines resulted in reduced proliferation and migration. RNA sequencing revealed significant alterations in the Hippo pathway. Overexpression of CCDC25 inhibited the expression of downstream Hippo pathway proteins ITGA3 and CCND1 and promoted YAP phosphorylation. Mechanistic studies showed that CCDC25 interacts with YAP and influences YAP phosphorylation through LATS1. In vivo, CCDC25 overexpression inhibited tumor growth and promoted apoptosis. Conclusion CCDC25 acts as a potential tumor suppressor in ccRCC by inhibiting cell proliferation and migration, potentially through regulating the Hippo signaling pathway. These findings highlight the potential of CCDC25 as a therapeutic target in ccRCC treatment. Graphical Abstract

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Section: Methodsmentioning

confidence: 99%

CCDC25 suppresses clear cell renal cell carcinoma progression by LATS1/YAP-mediated regulation of the hippo pathway

Tan,

Liu,

et al. 2024

Cancer Cell Int

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“…Targeted therapies and immunotherapies are crucial modalities for managing KIRC. 39 First, drug sensitivity to several commonly used targeted therapies was calculated using the pRRophetic algorithm. Furthermore, we performed investigations to examine the relationship between TNNT1 levels of expression and responsiveness to specific treatment, as indicated by IC50 measurements.…”

Section: Methodsmentioning

confidence: 99%

“…Targeted therapies and immunotherapies are crucial modalities for managing KIRC 39 . First, drug sensitivity to several commonly used targeted therapies was calculated using the pRRophetic algorithm.…”

Section: Methodsmentioning

confidence: 99%

Troponin T1 in tumorigenesis and immune modulation: Insights into multiple cancers and kidney renal clear cell carcinoma

Zhang,

Hao,

Wang

et al. 2024

J Cellular Molecular Medi

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Troponin T1 (TNNT1) plays a crucial role in muscle contraction but its role in cancer, particularly in kidney renal clear cell carcinoma (KIRC), is not well‐understood. This study explores the expression, clinical significance and biological functions of TNNT1 in various cancers, with an emphasis on its involvement in KIRC. We analysed TNNT1 expression in cancers using databases like TCGA and GTEx, assessing its prognostic value, mutation patterns, methylation status and functional implications. The study also examined TNNT1's effect on the tumour microenvironment and drug sensitivity in KIRC, complemented by in vitro TNNT1 knockdown experiments in KIRC cells. TNNT1 is overexpressed in several cancers and linked to adverse outcomes, showing frequent upregulation mutations and abnormal methylation. Functionally, TNNT1 connects to muscle and cancer pathways, affects immune infiltration and drug responses, and its overexpression in KIRC is associated with advanced disease and reduced survival. Knocking down TNNT1 curbed KIRC cell growth. TNNT1's aberrant expression plays a significant role in tumorigenesis and immune modulation, highlighting its value as a prognostic biomarker and a potential therapeutic target in KIRC and other cancers. Further studies are essential to understand TNNT1's oncogenic mechanisms in KIRC.

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Immune-pyroptosis-related genes predict the prognosis of kidney renal clear cell carcinoma

Cited by 2 publications

References 43 publications

CCDC25 suppresses clear cell renal cell carcinoma progression by LATS1/YAP-mediated regulation of the hippo pathway

CCDC25 suppresses clear cell renal cell carcinoma progression by LATS1/YAP-mediated regulation of the hippo pathway

Troponin T1 in tumorigenesis and immune modulation: Insights into multiple cancers and kidney renal clear cell carcinoma

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