Immune Reactions to &lt;i&gt;Nippostrongylus brasiliensis &lt;/i&gt;in the Rat

Keller, R.

doi:10.1159/000230233

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Immunoglobulins and Helminth Infections1

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Cited by 12 publications

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References 23 publications

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“…Although reagins are continuously present in the circulation, sensitivity to systemic anaphylaxis during a course of infections in rats with N. brasiliensis is not directly correlated with the presence of reagins (Ogilvie 1967, Keller 1969. Why do helminths (and probably ectoparasites?)…”

Section: Immunoglobulins and Helminth Infectionsmentioning

confidence: 99%

Immunity: Host Response

1970

The Journal of Parasitology

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Section: Immunoglobulins and Helminth Infectionsmentioning

confidence: 99%

Immunity: Host Response

1970

The Journal of Parasitology

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Antianaphylactic and Antiallergic Effects

Hahn¹

1978

Histamine II and Anti-Histaminics

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Changes in mast cell phenotype in rat lung following infection with Nippostrongylus brasiliensis

Yue

Pearce

2000

Inflamm. res.

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IntroductionPrevious studies by ourselves and others have shown that mast cells may undergo functional changes in the course of allergic and inflammatory disease [1]. Infection of the rat with the nematode parasite Nippostrongylus brasiliensis leads to an acute inflammatory response in the lung and intestine of the animal [2, 3]. We have now used this as a model system and have compared the properties of mast cells recovered from the peritoneum and by enzymatic dissociation of the lung, mesentery and prostate of normal and sensitised animals. Materials and methodsMale Sprague Dawley rats (ca 250 g) were used throughout. Lung, prostate and mesentery were dispersed into their component cells by treatment with the enzyme collagenase [4]. Peritoneal mast cells were obtained by direct lavage [4]. Where appropriate, animals were sensitised to N. brasiliensis and used two weeks later when the inflammatory response in the lung was maximal [5]. Histamine release was determined spectrofluorometrically as previously described [5]. Cytocentrifuge preparations of mast cells were fixed in either Carnoy's solution or formol saline and dried. The sides were then stained with alcian blue dye (30 min) and counterstained with safranin O (5 min) [6].The following secretagogues were purchased from Sigma unless otherwise noted: anti-rat IgE (ICN), compound 48/80, concanavalin A, dextran (RMM 70,000), histone, ionomycin (Calbiochem), ionophore A23187 and polymyxin B.

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Immune Reactions to <i>Nippostrongylus brasiliensis </i>in the Rat

Cited by 12 publications

References 23 publications

Immunity: Host Response

Immunity: Host Response

Antianaphylactic and Antiallergic Effects

Changes in mast cell phenotype in rat lung following infection with Nippostrongylus brasiliensis

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