Immune response and alteration of pulmonary function after primary human metapneumovirus (hMPV) infection of BALB/c mice

Darniot, Magali; Petrella, Tony; Aho, Serge; Pothier, Pierre; Manoha, Catherine

doi:10.1016/j.vaccine.2005.04.027

Cited by 39 publications

(39 citation statements)

References 29 publications

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“…In addition to Alvarez et al [1,2], Hamelin et al [6] observed that the lung virus peaks somewhat earlier at day 5 p.i. in both BALB/c mice and cotton rats, an observation that is supported by Darniot et al [4].…”

Section: Mouse Model (Balb/c)mentioning

confidence: 54%

“…Th2-type response, low levels of interferon-gamma (IFN-γ) production at all stages of infection, and delayed specific CTL response that coincides with persistent virus replication in the lung [2]. Weak interferon gamma production was also found in BALB/c mouse lung homogenates in other studies and peaked from days 5 to 7 [4][5][6]. Thereby, the work by Guerrero-Plata et al [5] showed that HMPV is a stronger inducer of both alpha interferon (IFN-α) and IFN-γ responses than RSV.…”

Section: Mouse Model (Balb/c)mentioning

confidence: 99%

“…Furthermore, the BALB/c mice infected with HMPV exhibited symptoms that included ruffled fur, huddling behavior and weight loss [4]. Thus, BALB/c mice appear to be a good model for HMPV replication, immunity and protection, but do not exhibit respiratory illness similar to that of humans and do not constitute an exact "disease" model.…”

Section: Mouse Model (Balb/c)mentioning

confidence: 99%

“…The reasons for this discussion were two earlier reports from Macphail et al [12] and Williams et al [23] that demonstrated that mice are not permissive for HMPV, although the usefulness of the model was supported by the data of Hamelin et al [6][7][8] as well as the recent observations from Darniot et al [4] who investigated immunological response against the HMPV infection in infected BALB/c mice [4,[6][7][8] and cotton rats [6], respectively. It is noteworthy that Darniot et al [4] observed HMPV replication of 10 3 pfu/g in the lungs of BALB/c mice, similar to levels detected by Williams et al [23] and thus substantially lower than the levels of lung replication detected in BALB/c mice by Alvarez et al [1,2] and Hamelin et al [6][7][8]. A number of factors may account for these divergent findings.…”

Section: Mouse Model (Balb/c)mentioning

confidence: 99%

“…Several animal species have been determined to be permissive for HMPV infection [1,4,6,9,10,12,18,23,27]. These models, which are described in more detail below, include small animals such as mice, cotton rats, hamsters, guinea pigs, ferrets, and primates, including chimpanzees, rhesus macaques and African green monkeys.…”

Section: Introductionmentioning

confidence: 99%

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Animal models for human Metapneumovirus (HMPV) infections

2007

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-Since its detection in 2001 the human Metapneumovirus (HMPV), a member of the Paramyxoviridae family, was observed to be a serious pathogen in human respiratory infections during childhood. Meanwhile, several animal models have been established to study the virus-host interactions and pathogenic effects. Mainly, small laboratory animals like mice and cotton rats have been used, although the usage of these two species for HMPV research is controversially discussed and contradictious results were obtained by different groups. Further trials with ferrets, hamsters and non human primates were performed revealing different success in their individual usage. In this review we present the different animal models, summarize their advantages and disadvantages, and discuss the controversial results from different studies.

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Section: Mouse Model (Balb/c)mentioning

confidence: 54%

Section: Mouse Model (Balb/c)mentioning

confidence: 99%

Section: Mouse Model (Balb/c)mentioning

confidence: 99%

Section: Mouse Model (Balb/c)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Animal models for human Metapneumovirus (HMPV) infections

2007

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Immunogenicity in mice of human metapneumovirus with a truncated SH glycoprotein

Tedcastle

Fenwick

Robinson

et al. 2013

Journal of Medical Virology

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The SH glycoprotein of human metapneumovirus (HMPV) is twice the size of that of human respiratory syncytial virus and possesses a large, hydrophilic luminal domain. The glycoprotein is located on the surface of the virion and of virus infected cells and, if immunogenic, might be expected to play a role in anti-viral immunity. Initial attempts to study anti-SH antibody immunogenicity were thwarted by the instability of the SH gene on passage both in human bronchial epithelial cells and in mice. Repeated passage of virus isolates in human bronchial epithelial cells in culture resulted in the appearance and eventual predominance of HMPV mutants lacking all or most of the luminal domain of SH coincidental with the loss of productive infection in mouse lungs. Where infection was established in mice with an early cell culture passage, the virus recovered from mouse lung differed markedly from the inoculum, carrying 19 coding mutations in the SH luminal domain. Immunization of mice with a mutant virus variant expressing only 14 amino acids of the luminal domain of SH induced a cross-reactive antibody response to both the F glycoprotein and the SH glycoprotein but a largely sub-group specific response to the G glycoprotein. Similar patterns of response were achieved by immunization with individual HMPV glycoproteins expressed from recombinant vaccinia viruses. Recombinant truncated SH glycoprotein induced sub-group cross-reactive antibodies capable of neutralizing wild-type virus. Recombinant F glycoprotein also induced cross-reactive neutralizing antibodies whilst recombinant G glycoprotein induced largely strain-specific, non-neutralizing antibodies.

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Human metapneumovirus infection

Warris

Groot

2007

Pediatric Infectious Diseases Revisited

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Immune response and alteration of pulmonary function after primary human metapneumovirus (hMPV) infection of BALB/c mice

Cited by 39 publications

References 29 publications

Animal models for human Metapneumovirus (HMPV) infections

Animal models for human Metapneumovirus (HMPV) infections

Immunogenicity in mice of human metapneumovirus with a truncated SH glycoprotein

Human metapneumovirus infection

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