Immune response and mitosis of human peripheral blood lymphocytes in vitro

The stimulation of DNA synthesis in cultures of rabbit lymph node and spleen cell suspensions by homologous cells

“…The response described here would appear to be analogous to the findings of Bain et al (10,11) and HirschhoIn and his colleagues (12) on the mixing of cultures of human peripheral leukocytes.…”

“…This response would appear to be similar to that noted in mixed cultures of human peripheral leukocytes described earlier by Bain et al (10,11) and I-Iirschhorn et al (12).…”

“…These and other workers have demonstrated similar in vitro changes in peripheral leukocytes brought about by antigens to which the subject had been immunized (17)(18)(19)(20)(21)(22)(23)(24)(25)(26), by extracts of autologous tissues in patients with autoimmune diseases (15,26,27), antisera from rabbits immunized to human leukocytes (28,29), and by phytohemaggiutinin M (12,25,(30)(31)(32)(33)(34)(35)(36)(37). The response to the latter was found to be most intense.…”

“…Hirschhorn and his colleagues (12,13) have described similar findings. In their experiments, as many as 50 to 70 per cent of the cells were large "transformed" cells in mixed cultures observed at 4 and 7 days, but there is no dear indication whether these ceils represent the transformation of a major fraction of the original ceils or are the progeny of a much smaller fraction stimulated to rapid proliferation.…”

Hepatotoxicity Due to Pemoline

“…Lymphocytes for transformation studies were cultured by the method of H irschhorn et al [7]. Lymphocyte stimulation was measured as incorporation of "H-thymidinc into DNA of lymphocytes using the method of Robbins et at.…”