Immune response and protective immunity after vaccination of Atlantic salmon (Salmo salar L.) against furunculosis

A colorimetric assay for the quantification of brook trout (Salvelinus fontinalis) lymphocyte mitogenesis

“…Ten microlitres of mitogen solution were added per well with the final concentrations being 200 or 500 g ml 1 for LPS, 10 g ml 1 for PHA and 25 g ml 1 for ConA. These mitogen doses had previously been optimised in preliminary experiments with brook trout which were based on previous studies with salmonid lymphocytes (DeKoning & Kaattari, 1991;Erdal & Reitan, 1992).…”

“…Aeromonas salmonicida strain 80204 (Olivier, 1990) was grown on TSA for 48 h at 22 C. Bacteria were killed with 0·5% formalin, washed twice in 0·85% saline and 10 l was added to wells at a final concentration of 10 8 bacteria ml 1 , as described by Erdal & Reitan (1992).…”

Pathology of Atlantic salmon Salmo salar intraperitoneally immunized with oil-adjuvanted vaccine. A case report

“…There can be little doubt that the most significant factor in reducing outbreaks is the extensive vaccination programs that have been carried out. In particular, the introduction of oil-adjuvanted vaccines for immunization against furunculosis and other septicaemic diseases caused by Gram-negative bacteria has shown good effect and reduced the occurrence of furunculosis both experimentally (Erdal & Reitan 1992 and in the field (Midtlyng 1996).…”

Evaluation of a cohabitation challenge model in immunization trials for channel catfish Ictalurus punctatus against Ichthyophthirius multifiliis

“…Cohabitation challenges have been used in studies for evaluating protective immunity of vaccinated fish against bacteria (Erdal & Reitan 1992, Klesius et al 2006) and viruses (Raynard et al 2001). In a recent study, sham-vaccinated Nile tilapia marked with calcein were cohabited with vaccinated tilapia and then challenged to evaluate the efficacy of vaccination (Klesius et al 2006).…”