Immune response and susceptibility to Cotesia flavipes parasitizing Diatraea saccharalis larvae exposed to and surviving an LC25 dosage of Bacillus thuringiensis

Pinto, Ciro Pedro Guidotti; Azevedo, Emiliano Brandão de; Santos, Ana Letícia Zéro dos; Cardoso, Camila Pires; Fernandes, Fabrício Oliveira; Rossi, Gabriel Augusto Marques; Polanczyk, Ricardo Antônio

doi:10.1016/j.jip.2019.107209

Cited by 13 publications

(3 citation statements)

References 48 publications

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“…82 Likely, in our study, MbNPV was transmitted to FAW larvae and progeny of C. marginiventris through parasitism and caused transgenerational effects. Because Bt and baculovirus can share hosts with the parasitoids and regulate host immunity, respectively, 84,85 the interactions between bioinsecticides and parasitoids are more complex. In addition, in our study, Bt and MbNPV caused hormesis effects similar to those of the three chemical agents, with neurotoxic effects on the parasitoid.…”

Section: Discussionmentioning

confidence: 99%

Transgenerational hormesis and sublethal effects of five key insecticides for controlling Spodoptera frugiperda on its endoparasitoid Cotesia marginiventris

Hou,

Zang,

Lü

et al. 2023

Pest Management Science

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BACKGROUNDThe endoparasitoid Cotesia marginiventris (Cresson) is a promising biological control agent of the fall armyworm (FAW) Spodoptera frugiperda (Smith). Because the application of insecticides is one of the prime choices in pest management, we evaluated the sublethal and transgenerational effects of the five key insecticides—chlorantraniliprole, emamectin benzoate, spinetoram, Bacillus thuringiensis (Bt), and Mamestra brassicae nucleopolyhedrovirus (MbNPV)—on the parasitoid.ResultsExposure to five insecticides at a concentration causing 10% mortality (LC10) caused hormetic effects in the parent generation (F0) by increasing the parasitism and reducing the immature duration. Interestingly, the hormetic response was also observed in the offspring generation indirectly exposed to the insecticides. Furthermore, insecticides increased the parasitism rate by 6.32–14.73% in the F1 generation, which was similar to that of the F0 generation (3.96–11.81%) compared with the control. No significant adverse effect was observed on the number of emerged parasitoids of the F1 and F2 generations. However, insecticides had a detrimental impact on body size and fecundity in the F1 and F2 generations, which showed a small body size with shorter hind tibiae and a significant reduction in the female ratio compared with the control; the exception was that chlorantraniliprole significantly improved the female ratio in the F2 generation.ConclusionsFive insecticides at LC10 induced transgenerational hormetic and sublethal effects on C. marginiventris. Our results provide a scientific basis for a better understanding of the long‐term impacts of insecticides at sublethal doses on parasitoids, facilitating the development of improved integrated pest management programs for FAW control. © 2023 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Transgenerational hormesis and sublethal effects of five key insecticides for controlling Spodoptera frugiperda on its endoparasitoid Cotesia marginiventris

Hou,

Zang,

Lü

et al. 2023

Pest Management Science

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“…Considering the importance of the parasitoid C. avipes for the management of D. saccharalis [7], it is noteworthy evaluate the possible impacts of D. saccharalis with increased phenol oxidase activity over the parasitism success of C. avipes. However, these effects may be null or minimum given that D. saccharalis larvae with increased values for phenol oxidase activity stimulated by exposition to B. thuringiensis were still susceptible to parasitism by C. avipes without noticeable impacts over the development of the parasitoid [27]. Moreover, eld evaluation of Si-fertilized sugarcane plants also indicated compatibility of Si-fertilized plants with the use of C. avipes for the management of D. saccharalis [65].…”

Section: Discussionmentioning

confidence: 99%

Silicon Application Enhances Sugarcane Growth by Impairing the Development of Larval Sugarcane Borer

Sousa,

Pinto,

dos Santos

et al. 2023

Silicon

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Sugarcane borer, Diatraea saccharalis, is one of the main insect pests of sugarcane elds, and it has been mainly managed by the use of chemical or biological controls. Considering the bene ts of Silicon (Si) fertilization against pests, it was assessed the development of sugarcane borer larvae and sugarcane growth with and without Si. A greenhouse experiment was conducted using a factorial design (2x2) with 10 repetitions. Two commercial sugarcane varieties were evaluated: SP80-3280 and IAC91-1099, which has, respectively, susceptibility, and intermediate resistance to D. saccharalis. Si was applied in soil in an equivalent rate of 800 kg of Si ha − 1 . Before herbivory, Si increased stalk diameter and plant height in both varieties, and number of leaves and leaf width were only increased in IAC91-1099. After 20 days of herbivory, Si increased stalk diameter in both varieties and plant height in IAC91-1099, but decreased the number of leaves and leaf width in SP80-3280. Larval D. saccharalis showed a reduced weight and a greater index for mandible abrasion after feeding Si-treated plants independently of variety. No in uence of Si-treated plants was found in immunological parameters of larvae (total number of hemocytes, cell viability, encapsulation capability, lysozyme active). The activity of phenol oxidase, an immunological and stress marker for insects, was greater in larval D. saccharalis fed with IAC 91-1099, independently of Si. In conclusion, Si application improved sugarcane growth of IAC91-1099 and impaired the development of larval D. saccharalis in both sugarcane varieties.

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“…Nevertheless, insects have a chance of survival after Bt infection. Immune responses to sublethal doses of Bt have been found to reduce bacterial pathogenicity, which may accelerate the development of resistance [5][6][7][8][9]. Identifying insect factors that interfere with Bt pathogenesis may help to develop new formulations with increased toxicity.…”

Section: Introductionmentioning

confidence: 99%

RNA Interference-Mediated Knockdown of Bombyx mori Haemocyte-Specific Cathepsin L (Cat L)-Like Cysteine Protease Gene Increases Bacillus thuringiensis kurstaki Toxicity and Reproduction in Insect Cadavers

Yang

Sun

Chang

et al. 2022

Toxins

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The silkworm’s Cat L-like gene, which encodes a lysosomal cathepsin L-like cysteine protease, is thought to be part of the insect’s innate immunity via an as-yet-undetermined mechanism. Assuming that the primary function of Cat L-like is microbial degradation in mature phagosomes, we hypothesise that the suppression of the Cat L-like gene expression would increase Bacillus thuringiensis (Bt) bacteraemia and toxicity in knockdown insects. Here, we performed a functional analysis of Cat L-like in larvae that were fed mulberry leaves contaminated with a commercial biopesticide formulation based on Bt kurstaki (Btk) (i.e., Dipel) to investigate its role in insect defence against a known entomopathogen. Exposure to sublethal doses of Dipel resulted in overexpression of the Cat L-like gene in insect haemolymph 24 and 48 h after exposure. RNA interference (RNAi)-mediated suppression of Cat L-like expression significantly increased the toxicity of Dipel to exposed larvae. Moreover, Btk replication was higher in RNAi insects, suggesting that Cat L-like cathepsin may be involved in a bacterial killing mechanism of haemocytes. Finally, our results confirm that Cat L-like protease is part of the antimicrobial defence of insects and suggest that it could be used as a target to increase the insecticidal efficacy of Bt-based biopesticides.

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Immune response and susceptibility to Cotesia flavipes parasitizing Diatraea saccharalis larvae exposed to and surviving an LC25 dosage of Bacillus thuringiensis

Cited by 13 publications

References 48 publications

Transgenerational hormesis and sublethal effects of five key insecticides for controlling Spodoptera frugiperda on its endoparasitoid Cotesia marginiventris

Transgenerational hormesis and sublethal effects of five key insecticides for controlling Spodoptera frugiperda on its endoparasitoid Cotesia marginiventris

Silicon Application Enhances Sugarcane Growth by Impairing the Development of Larval Sugarcane Borer

RNA Interference-Mediated Knockdown of Bombyx mori Haemocyte-Specific Cathepsin L (Cat L)-Like Cysteine Protease Gene Increases Bacillus thuringiensis kurstaki Toxicity and Reproduction in Insect Cadavers

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