Immune response gene coexpression network analysis of Arachis hypogaea infected with Aspergillus flavus

Jayaprakash, Aiswarya; Roy, Abhijeet Deb; Thanmalagan, Raja Rajeswary; Arunachalam, Annamalai; Lakshmi, P.T.V.

doi:10.1016/j.ygeno.2021.06.027

Cited by 10 publications

(5 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is involved in the production of phytohormones, metabolites, growth, development, and biotic and abiotic stress ( Li et al., 2014 ; Rehman et al., 2018 ). UDP members of the GT1 family catalyze, help the biosynthesis of oligo- and polysaccharides, and transfer sugar residues from nucleotide donor substrates to receptor substrates or a developing carbohydrate chain ( Hoffmeister et al., 2001 ; Jayaprakash et al., 2021 ). GT1-related genes were substantially up-regulated in the Fg-affected silk of Z. mays .…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome profiling and weighted gene co-expression network analysis to identify core genes in maize (Zea mays L.) silks infected by multiple fungi

Kumar

Kanak²,

Arunachalam³

et al. 2022

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Maize (Zea mays L.) is the third most popular Poaceae crop after wheat and rice and used in feed and pharmaceutical sectors. The maize silk contains bioactive components explored by traditional Chinese herbal medicine for various pharmacological activities. However, Fusarium graminearum, Fusarium verticillioides, Trichoderma atroviride, and Ustilago maydis can infect the maize, produce mycotoxins, hamper the quantity and quality of silk production, and further harm the primary consumer’s health. However, the defense mechanism is not fully understood in multiple fungal infections in the silk of Z. mays. In this study, we applied bioinformatics approaches to use the publicly available transcriptome data of Z. mays silk affected by multiple fungal flora to identify core genes involved in combatting disease response. Differentially expressed genes (DEGs) were identified among intra- and inter-transcriptome data sets of control versus infected Z. mays silks. Upon further comparison between up- and downregulated genes within the control of datasets, 4,519 upregulated and 5,125 downregulated genes were found. The DEGs have been compared with genes in the modules of weighted gene co-expression network analysis to relevant specific traits towards identifying core genes. The expression pattern of transcription factors, carbohydrate-active enzymes (CAZyme), and resistance genes was analyzed. The present investigation is supportive of our findings that the gene ontology, immunity stimulus, and resistance genes are upregulated, but physical and metabolic processes such as cell wall organizations and pectin synthesis were downregulated respectively. Our results are indicative that terpene synthase TPS6 and TPS11 are involved in the defense mechanism against fungal infections in maize silk.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome profiling and weighted gene co-expression network analysis to identify core genes in maize (Zea mays L.) silks infected by multiple fungi

Kumar

Kanak²,

Arunachalam³

et al. 2022

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Importantly, two TLPs—sweet potato LbACP ( Lpomea batatas anti-cancer peptide) and European plum ( Prunus domestica L.) PdPR5-1—were recently discovered to be HDPs, and they may provide us with valuable tools for developing phytosanitary products [ 71 ]. Regarding legumes, the peanut gene TLP1b is a candidate resistant gene that may be used to impart an immune response to Aspergillus flavus infections [ 72 ]. However, little is known about legume TLPs in terms of plant immune responses.…”

Section: Biological Functions Of Tlps In Legumesmentioning

confidence: 99%

Thaumatin-like Proteins in Legumes: Functions and Potential Applications—A Review

Feng,

Wei,

2024

Plants

View full text Add to dashboard Cite

Thaumatin-like proteins (TLPs) comprise a complex and evolutionarily conserved protein family that participates in host defense and several developmental processes in plants, fungi, and animals. Importantly, TLPs are plant host defense proteins that belong to pathogenesis-related family 5 (PR-5), and growing evidence has demonstrated that they are involved in resistance to a variety of fungal diseases in many crop plants, particularly legumes. Nonetheless, the roles and underlying mechanisms of the TLP family in legumes remain unclear. The present review summarizes recent advances related to the classification, structure, and host resistance of legume TLPs to biotic and abiotic stresses; analyzes and predicts possible protein–protein interactions; and presents their roles in phytohormone response, root nodule formation, and symbiosis. The characteristics of TLPs provide them with broad prospects for plant breeding and other uses. Searching for legume TLP genetic resources and functional genes, and further research on their precise function mechanisms are necessary.

show abstract

“…A. flavus is known to produce aflatoxins on various crops, including corn, peanuts, cottonseed, and nuts, resulting in significant agricultural economic losses and posing health risks [37,38]. The pathogenicity of ATG8 on crop seeds was detected.…”

Section: Effect Of Atg8 Mutants On Pathogenicity To Crop Seedsmentioning

confidence: 99%

The Autophagy-Related Protein ATG8 Orchestrates Asexual Development and AFB1 Biosynthesis in Aspergillus flavus

Geng,

Hu,

et al. 2024

JoF

View full text Add to dashboard Cite

Autophagy, a conserved cellular recycling process, plays a crucial role in maintaining homeostasis under stress conditions. It also regulates the development and virulence of numerous filamentous fungi. In this study, we investigated the specific function of ATG8, a reliable autophagic marker, in the opportunistic pathogen Aspergillus flavus. To investigate the role of atg8 in A. flavus, the deletion and complemented mutants of atg8 were generated according to the homologous recombination principle. Deletion of atg8 showed a significant decrease in conidiation, spore germination, and sclerotia formation compared to the WT and atg8Cstrains. Additionally, aflatoxin production was found severely impaired in the ∆atg8 mutant. The stress assays demonstrated that ATG8 was important for A. flavus response to oxidative stress. The fluorescence microscopy showed increased levels of reactive oxygen species in the ∆atg8 mutant cells, and the transcriptional result also indicated that genes related to the antioxidant system were significantly reduced in the ∆atg8 mutant. We further found that ATG8 participated in regulating the pathogenicity of A. flavus on crop seeds. These results revealed the biological role of ATG8 in A. flavus, which might provide a potential target for the control of A. flavus and AFB1 biosynthesis.

show abstract

Immune response gene coexpression network analysis of Arachis hypogaea infected with Aspergillus flavus

Cited by 10 publications

References 67 publications

Comparative transcriptome profiling and weighted gene co-expression network analysis to identify core genes in maize (Zea mays L.) silks infected by multiple fungi

Comparative transcriptome profiling and weighted gene co-expression network analysis to identify core genes in maize (Zea mays L.) silks infected by multiple fungi

Thaumatin-like Proteins in Legumes: Functions and Potential Applications—A Review

The Autophagy-Related Protein ATG8 Orchestrates Asexual Development and AFB1 Biosynthesis in Aspergillus flavus

Contact Info

Product

Resources

About