Immune response in Lutjanus erythropterus induced by the major outer membrane protein (OmpU) of Vibrio alginolyticus

Abstract

The outer membrane proteins (OMPs) of the marine aquatic animal pathogen Vibrio alginolyticus play an important role in the virulence of the bacterium and are potential candidates for vaccine development. In this study, the major 35.6 kDa OMP of V. alginolyticus was isolated by gel excision from the crude OMP fraction from V. alginolyticus. The sequence of the first 27 amino acid residues from the N-terminal end of the protein is ATV YKD GGT ELL VGG RVE FRG DFI GSD, which has high homology with OmpU proteins from other Vibrio spp. (92%). Lutjanus erythropterus were vaccinated with OmpU, and immunogenicity was confirmed by subsequent western blotting. Enzyme-linked immunosorbent assay (ELISA) analysis demonstrated that OmpU produced an observable antibody response in all sera of the vaccinated fish. L. erythropterus vaccinated with OmpU produced specific antibodies, and were highly resistant to infection with virulent V. alginolyticus. These results indicate that OmpU is an effective vaccine candidate against V. alginolyticus for L. erythropterus. KEY WORDS: Vibrio alginolyticus · Outer membrane protein · OmpU · Immunogenicity · VaccineResale or republication not permitted without written consent of the publisher

Cloning, expression of Vibrio alginolyticus outer membrane protein‐OmpU gene and its potential application as vaccine in crimson snapper, Lutjanus erythropterus Bloch

“…They play multiple functions and roles that help bacteria survive, such as acting as antennae for environmental signals, adhesion for colonization and also as invasion mediators (Cai et al . ). OMPs extracted from some bacterial fish pathogens have been shown to be protective antigens, including V. alginolytiucs , V. anguillarum , V. harveyi , Aeromonas hydrophila , Aeromonas veronii , Aliivibrio salmonicida and Edwardsiella tarda in fish (Vazquez‐Juarez et al .…”

“…In a previous study, we reported that the isolated OmpU was a good protective antigen of f V. alginolyticus in L. erythropterus (Cai et al . ). It was also reported that the OmpU in V. cholerae V. fischeri and V. splendidus could provide significantly protection against corresponding pathogens (Das et al .…”

“…; Cai et al . ). Due to diversity of the pathogens and their serotypes, the progress in the development of a vaccine against vibriosis has been slow.…”

“…Recently, we found the isolated OmpU protein of V. alginolyticus could protect L. erythropterus from the infection of virulent V. alginolyticus (Cai et al . ). To explore the potential application of the recombinant OmpU as a vaccine candidate for L. erythropterus , the present study was proceeded with the cloning and expression of OmpU gene of V. alginolyticus and evaluates immunogenicity of the recombinant proteins in L. erythropterus .…”

Immunogenicity study of an expressed outer membrane protein U ofVibrio vulnificusin Japanese eel (Anguilla japonica)

“…This result agreed with the protective effect of OmpU of V. alginolyticus and V. mimicus (Cai et al . ; Cen et al . ).…”