Immune Response of Guinea Pigs to Common Enterobacterial Antigen

Enterobacterial common antigen.

“…Guinea pigs have been immunized by both E. coli 014 extracts and ethanol-soluble ECA in Freund adjuvant and tested for cutaneous delayed hypersensitivity and antibody production (132). No or only minimal anti-ECA activity was detected in the sera 9 days after a single dose of immunogen.…”

Chemistry and Biology of the Enterobacterial Common Antigen (ECA)

“…Hartley albino guinea-pigs were immunized by Morgenstern and Gorzynski (1973) with ECA extracted from heated bacterial suspensions (E. coli 014 and 0111) by meanS of 85% ethanol. ECA was administered with complete Freund's adjuvant into the four footpads of the animals.…”

Immunogenic cross-reactivity between human tissues and the enterobacterial common antigen

“…However, with the exception of Escherichia coli 014 and R-1 and R-4 core types (11,26), ECA has been shown to be non-immunogenic in the presence of its own or heterologous lipopolysaccharide (LPS) or lipid A (23,27). Ethanol-soluble ECA, essentially free from LPS, is capable of engendering significant ECA hemagglutinin levels in rabbits (17), human subjects (8), and, to a lesser degree, guinea pigs (15). Although LPS appears to suppress humoral ECA antibody formation, i.e., animals given a mixture of ECA 1 Present address: Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611.…”

“…on days 0, 3, 6, 9, and 16. Sera were obtained on days 0, 12, 20, and 23 and titrated for ECA hemagglutinins by a procedure previously described (15).…”

“…In addition, the effects of our tissue preparations on the modifying capacity of bacteria-derived ECA were examined. To this end, individual or combined preparations of human kidney and E. coli 0111, as described below, were used to passively modify and/or inhibit ECA hemagglutination with anti-S. typhimurium, using a microtiter technique (15). E. coli 0111 HKS, E. coli 0111 ES, human kidney HE, and human kidney ES preparations were obtained by procedures previously described.…”