Immune response of silver pomfret (<i>Pampus argenteus</i>) to <i>Amyloodinium ocellatum</i> infection

Zhang, Youyi; Hu, Jiabao; Li, Yaya; Zhang, Man; Jacques, Kimran Jean; Gu, Weiwei; Sun, Yibo; Sun, Jiachu; Yang, Yang; Xu, Shanliang; Wang, Yajun; Yan, Xiaojun

doi:10.1111/jfd.13524

Cited by 33 publications

(6 citation statements)

References 62 publications

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“…Previous studies revealed that A. ocellatum infection induces a potent innate immune response in the host, including increased expression of genes linked to apoptosis and pro‐inflammation (Byadgi et al., 2019, 2021; Li et al., 2022; Zhang et al., 2021). In this view, Casp 3 , IL‐1 , IL‐10 and TNF‐α were selected to assess whether an in vitro platform could be used as a surrogate to examine parasite–host interactions.…”

Section: Discussionmentioning

confidence: 99%

Establishment of a gill cell line from yellowfin seabream (Acanthopagrus latus) for studying Amyloodinium ocellatum infection of fish

Li,

Zhuang,

Chen

et al. 2024

Journal of Fish Diseases

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Amyloodinium ocellatum is among the most devastating protozoan parasites, causing huge economic losses in the mariculture industry. However, the pathogenesis of amyloodiniosis remains unknown, hindering the development of targeted anti‐parasitic drugs. The A. ocellatum in vitro model is an indispensable tool for investigating the pathogenic mechanism of amyloodiniosis at the cellular and molecular levels. The present work developed a new cell line, ALG, from the gill of yellowfin seabream (Acanthopagrus latus). The cell line was routinely cultured at 28°C in Dulbecco's modified Eagle medium (DMEM) supplemented with 15% fetal bovine serum (FBS). ALG cells were adherent and exhibited an epithelioid morphology; the cells were stably passed over 30 generations and successfully cryopreserved. The cell line derived from A. latus was identified based on partial sequence amplification and sequencing of cytochrome B (Cyt b). The ALG was seeded onto transwell inserts and found to be a platform for in vitro infection of A. ocellatum, with a 37.23 ± 5.75% infection rate. Furthermore, scanning electron microscopy (SEM) revealed that A. ocellatum parasitizes cell monolayers via rhizoids. A. ocellatum infection increased the expression of apoptosis and inflammation‐related genes, including caspase 3 (Casp 3), interleukin 1 (IL‐1), interleukin 10 (IL‐10), tumour necrosis factor‐alpha (TNF‐α), in vivo or in vitro. These results demonstrated that the in vitro gill cell monolayer successfully recapitulated in vivo A. latus host responses to A. ocellatum infection. The ALG cell line holds great promise as a valuable tool for investigating parasite–host interactions in vitro.

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Section: Discussionmentioning

confidence: 99%

Establishment of a gill cell line from yellowfin seabream (Acanthopagrus latus) for studying Amyloodinium ocellatum infection of fish

Li,

Zhuang,

Chen

et al. 2024

Journal of Fish Diseases

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“…Fish were manually fed two times a day at 08:00 a.m. and 17:00 p.m. with commercial feed (larvae love 6#, Japan), until satiety. The experimental condition was controlled according to a previous study [41]. Throughout the experiment, the water temperature ranged between 25.5 • C and 27.5 • C, salinity was 24 ± 0.89%, and dissolved oxygen was 7.3 ± 0.49 mg/L.…”

Section: Fishmentioning

confidence: 99%

Molecular Characterization and Expression Response of Ghrelin, GLP-1 and PYY to Fasting, Dietary Lipid, and Fatty Acids in Silver Pomfret (Pampus argenteus)

Zhang

Shi

Zhang

et al. 2023

Fishes

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Ghrelin, glucagon-like peptide-1 (GLP-1), and peptide YY (PYY) are potent hormones mediating food intake according to the nutritional status in fish. However, limited information is available on these genes and their expression in response to nutrition in silver pomfret (Pampus argenteus). A comparison analysis revealed that ghrelin and GLP-1 were relatively conserved in marine fish. PYYa and PYYb shared a low identity and were clustered to different PYY branches. Ghrelin, pyya, and pyyb mRNAs were highly expressed in the brain, while glp-1 was highly expressed in the gills and liver. The ghrelin mRNA expression was relatively high an hour post-feeding and decreased after 3 to 72 h of fasting. The glp-1 mRNA expression was increased after 6 to 24 h of fasting. The pyya mRNA expression was increased after 72 h of fasting, while the pyyb expression remained stable during fasting. The diet with 14% lipid promoted the expressions of glp-1, pyya, and pyyb, but inhibited the ghrelin expression. The ghrelin expression in the intestine was increased after palmitic acid, oleic acid (OA), linoleic acid (LA), α-linolenic acid, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) incubation. OA decreased the expression of glp-1 and increased the expression of pyya. The expression of pyyb was downregulated by LA, DHA, and EPA. These results suggest the importance of ghrelin, glp-1, and pyy in coordinating food intake in response to fasting, dietary lipid concentration, and fatty acids in silver pomfret.

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“…There are also studies on a more global response of the organism to amyloodiniosis 102,214 and on the tomont phase of this parasite. 210 All these studies represent interesting results that can lead to the development of new therapies for amyloodiniosis.…”

Section: Omic's Approachesmentioning

confidence: 99%

Amyloodiniosis in aquaculture: A review

Moreira,

Costas,

Rodrigues

et al. 2023

Reviews in Aquaculture

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Fish ectoparasites are one of the pathogens groups that pose great concern to the aquaculture industry. The dinoflagellate Amyloodinium ocellatum is responsible for amyloodiniosis, a parasitological disease with a strong economic impact in temperate and warm water aquaculture, mainly in earthen pond semi‐intensive systems. Amyloodiniosis represents one of the most important bottlenecks for aquaculture and, with the predictable expansion of the area of influence of this parasite to higher latitudes due to global warming it might also be a threat to other aquaculture species that are not yet parasitized by A. ocellatum. This review made a compilation of the existing knowledge about this parasite and the disease associated with it. It was noticed that, except from the life cycle characterisation, detection methods, histopathological analysis, and treatments, there are still a lot of areas that need a further investment in research. Areas like parasite–host interactions, epidemiological models, taxonomy, host physiological responses to parasitism, and genome sequencing, amongst others, can contribute to a better understanding of this disease. These proposed approaches and routes of investigation will enhance and contribute to a more standardised knowledge, creating the opportunity for a better understanding of amyloodiniosis impacts on fish and contributing for the development of new tools against A. ocellatum, that may reduce fish mortality in aquaculture production due to amyloodiniosis outbreaks.

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Immune response of silver pomfret (Pampus argenteus) to Amyloodinium ocellatum infection

Cited by 33 publications

References 62 publications

Establishment of a gill cell line from yellowfin seabream (Acanthopagrus latus) for studying Amyloodinium ocellatum infection of fish

Establishment of a gill cell line from yellowfin seabream (Acanthopagrus latus) for studying Amyloodinium ocellatum infection of fish

Molecular Characterization and Expression Response of Ghrelin, GLP-1 and PYY to Fasting, Dietary Lipid, and Fatty Acids in Silver Pomfret (Pampus argenteus)

Amyloodiniosis in aquaculture: A review

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