Immune response to progesterone involved in Cu2+-mediated polyanion-protein complex — antigen specificity and affinity of hybridoma clones

Immune Response to Progesterone Immobilized on Cu2+-Induced Amphifilic Polyelectrolyte-Protein Complex: Antigen Specificity and Affinity of Hybridoma Clones

“…(5)(6)(7) Recently, we have shown the inclusion of steroid hormones (estradiol and progesterone) in water soluble polyelectrolyte-protein complexes. (8)(9)(10)(11) However, such polycomplexes were able to generate steroid (estradiole and progesterone) and BSA-specific antibodies, simultaneously. Of 60 progesteronereactive clones obtained in five fusions, 7 proved to be progesterone specific (K d 5 13.10 27 2 9.10 28 ) and of 80 estradiole-reactive clones obtained in three fusions, 10 proved to be estradiole-specific (K d 5 8.10 26 2 7.10 28).…”

Review on Assesment of Permeability of Enhancement Property of Hyaluronic Acid as Compare to Peg in Core Gel Liposome

“…Its tall deformability has been proposed to be the cause of their capacity to enter the skin and indeed permit for proteins to reach systemic circulation [69][70][71][72][73][74][75]. It is totally biocompatible additionally investigated by many specialists within the frame of polymeric microparticle and hydrogel for conveyance of protein and peptide [76][77][78][79][80][81].Gel center liposomes are the progressed liposomal build bearing center of biocompatible polymer interior the lipid vesicle. This system is the combination of polymer and lipid based delivery system, in which core of polymer serves function of skeleton and provides mechanical support to vesicles and in this way gelling will be actuated after division of unentrapped polymer.…”

Immune Response to 17β-Estradiol Involved in Polymer Gels: Antigen Specificity and Affinity of Hybridoma Clones

“…(9,10) We, and others, have previously shown that the attachment of weak microbial and viral protein antigens to various charged polymers of organic origin allows the modulation not only of their immunogenicity, but also protective activity. (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) Until now, formulations of some steroid hormones with polymers for controlled release have been reported, (21)(22)(23)(24)(25)(26)(27)(28)(29)(30) and these include microparticles of poly (lactic acid-co-glycolic acid), poly (lactide-co-epsilon-caprolactone), polyethylglycollactide copolymers containing levonergesterel and estradiol and water-soluble and insoluble polymer-hormone conjugates. (31)(32)(33)(34)(35)(36)(37)(38) However, there have been no reports of modifications to allow constructions of immunogenic polymer-steroid hormone conjugates.…”

“…(15)(16)(17)(18) However, such polycomplexes were able to generate steroid (estradiol or progesterone) and bovine serum albumin (BSA)-specific antibodies, simultaneously. Of 60 such progesterone-reactive clones obtained in 5 fusions, 7 proved to be progesterone specific (K d 5 13 3 10 27 -9 3 10 28 ) and of 80 estradiol-reactive clones obtained in three fusions, 10 proved to be estradiol-specific (K d 5 8 3 10 26 -7 3 10 28 ).…”