Immune response to the collagen-like synthetic ordered polypeptide (L-Pro-Gly-L-Pro)n

Maoz, Arieh; Fuchs, Sara; Sela, Michael

doi:10.1021/bi00745a031

Cited by 17 publications

(19 citation statements)

References 28 publications

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“…Further evidence that the antibody detection method might be successful was work with proline and glycine containing synthetic peptides used as immunogens, and the demonstration that antibody raised against these peptides cross-reacts with collagen (12 …”

Section: Discussionmentioning

confidence: 99%

Characterization of Carrot Cell Wall Protein

Smith¹

1981

Plant Physiol.

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Antiserum was raised against a synthetic nona-peptide which was predicted to have considerable homology with the unhydroxylated, unglycosylated precursor of cell wail proteins from several plants. The antiserum is able to recognize the major cell wail protein of incubated carrot (Daucus carota) root discs which is produced when the discs are treated with a proline hydroxylase inhibitor and then labeled with radioactive proline.This technique has potential applications in studying cell wail biosynthesis and its regulatory control mechanisms.Plant cell walls contain proteins rich in hydroxyproline (8).Traditional studies of these proteins use treatments which remove them from the wall, but which also break some of their peptide bonds (11). An approach which would obviate the need to destroy the polypeptide structure of the protein would be to study the protein prior to its incorporation into the cell wall. This approach requires a way to identify the protein in the presence of all the cytoplasmic proteins of the plant cell. Several criteria are available for making this identification; the protein should be rich in proline or hydroxyproline, should be transiently present in the cytoplasm, and should be absent (or present in a different form) when an inhibitor of proline hydroxylation is present.A more useful method would not only identify the protein, but also isolate it from the other cytoplasmic proteins. The method described in this paper has the potential to fulfill this need and is based on a striking characteristic of the peptides extracted from protease-treated tomato cell walls: five of the isolated peptides contain the sequence Ser-Hyp4 (9). The unusual nature of this peptide sequence suggested that it might serve as a unique marker of the cell wall protein, and that the sequence Ser-Pro4 might likewise serve as a marker for the unhydroxylated cell wall protein precursor. The method chosen to recognize the Ser-Pro4 sequence was to raise antibodies against a synthetic peptide containing the sequence. It was hoped that antibodies recognizing the synthetic peptide would also recognize the peptide sequence as it occurs in the cell wall protein precursor. MATERIALS AND METHODS

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Section: Discussionmentioning

confidence: 99%

Characterization of Carrot Cell Wall Protein

Smith¹

1981

Plant Physiol.

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“…Rabbits were immunized with the synthetic ordered polymer PGPn as described elsewhere [20]. Specific antibodies were isolated from rabbit antisera against PGPn by means of the i m mu no adso rben t (ProGl y-Pro) n-Sep haro se [ 201.…”

Section: Antisera and Antibodiesmentioning

confidence: 99%

Cytotoxic activity of antibodies to a collagen‐like synthetic polytripeptide on cells in tissue culture

Maoz

Dym

Fuchs³

et al. 1973

Eur J Immunol

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“…Borek et al [20] showed that (Pro-Gly-Proj., is immunogenic in guinea pigs and rabbits, and, using passive cutaneous anaphylaxis test, they demonstrated a weak cross-reaction with several collagens. Recently, we extended the immunological characterization of this ordered polymer, showing that it is an appropriate immunological model for collagen and thus can cross-react with natural collagens [21,22]. RESULTS …”

Section: Introductionmentioning

confidence: 99%

The Ordered Polymer (Pro‐Gly‐Pro)_n an Immunological Model for Collagen

Fuchs

Maoz

Sela

1974

Israel Journal of Chemistry

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The periodic collagen-like poly tripeptide (Pro-Gly-Proj., is immunogenic in several species of animals. Antibodies to this polymer are specific to the unique collagenlike conformation of the immunogen. There is an immunological cross-reactivity between (Pro-Gly-Projj, and natural collagens in several systems, and it is possible to induce an autoimmune response to collagen by means of the synthetic polymer. The ordered polymer (Pro-Gly-Projj, as well as collagen are thymus-independent antigens, whereas the random polymer (Pro·· ,Gly34)n and gelatin are thymus dependent.

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Immune response to the collagen-like synthetic ordered polypeptide (L-Pro-Gly-L-Pro)n

Cited by 17 publications

References 28 publications

Characterization of Carrot Cell Wall Protein

Characterization of Carrot Cell Wall Protein

Cytotoxic activity of antibodies to a collagen‐like synthetic polytripeptide on cells in tissue culture

The Ordered Polymer (Pro‐Gly‐Pro)_n an Immunological Model for Collagen

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Immune response to the collagen-like synthetic ordered polypeptide (L-Pro-Gly-L-Pro)n

Cited by 17 publications

References 28 publications

Characterization of Carrot Cell Wall Protein

Characterization of Carrot Cell Wall Protein

Cytotoxic activity of antibodies to a collagen‐like synthetic polytripeptide on cells in tissue culture

The Ordered Polymer (Pro‐Gly‐Pro)n an Immunological Model for Collagen

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Product

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The Ordered Polymer (Pro‐Gly‐Pro)_n an Immunological Model for Collagen