Immune responses and gene expression in white shrimp, Litopenaeus vannamei, induced by Lactobacillus plantarum

Chiu, Chiu‐Hsia; Guu, Yuan-Kuang; Liu, Chun-Hung; Pan, Tzu‐Ming; Cheng, Winton

doi:10.1016/j.fsi.2006.11.010

Cited by 345 publications

(216 citation statements)

References 49 publications

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“…Some studies suggest the same for marine shrimp. Chiu et al (2007) reported that feeding with L. plantarum supplemented diet modified some immunological parameters in L. vannamei including PO and THC. Li et al (2007) reported the same results for L. vannamei fed with Bacillus licheniformis supplemented diet to PO and THC.…”

Section: Resultsmentioning

confidence: 99%

Time-related action of Lactobacillus plantarum in the bacterial microbiota of shrimp digestive tract and its action as immunostimulant

Vieira

Neto

Mouriño

et al. 2008

Pesq. agropec. bras.

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-The objective of this work was to assess the time-related action of probiotic Lactobacillus plantarum in the bacterial microbiota of the digestive tract of Litopenaeus vannamei, and the relation of total haemocyte count and serum phenol oxidase activity of shrimp challenged with Vibrio harveyi. Shrimps were fed with a probiotic-supplemented diet, for eight days, then shifted to a commercial diet. Shrimps fed only with the commercial diet served as control. Evaluations were made on the 8 th day of experiment and repeated two, four, six and eight days later. Total lactic bacteria in the digestive tract was higher until the 4 th day of evaluation in the probioticsupplemented group. Vibrio spp. counts were higher in the control at days zero and two. Until the 4 th day of evaluation, the total haemocyte counts in shrimps after challenge with V. harveyi were higher in probioticsupplemented group than in control group. Significant difference was not observed in phenol oxidase activity. On the 6 th day after shifting from supplemented to control diet, all parameters were equal in both groups, suggesting that the time-related action of L. plantarum in shrimp is short.Index terms: Litopenaeus vannamei, Vibrio harveyi, total haemocyte count, lactic bacteria, phenol oxidase, probiotic. Tempo de atuação de Lactobacillus plantarum na microbiota bacteriana intestinal de camarão e sua ação como imunoestimulanteResumo -O objetivo deste trabalho foi avaliar o tempo de atuação de Lactobacillus plantarum, na microbiota bacteriana intestinal de Litopenaeus vannamei, e sua relação com a contagem total de hemócitos e a atividade da fenoloxidase após a exposição a Vibrio harveyi. Os camarões foram alimentados com dieta suplementada com probióticos por oito dias e, depois, substituída por dieta comercial. Os camarões alimentados com dieta comercial constituíram o controle. As avaliações foram realizadas no oitavo dia de experimento e repetidas dois, quatro, seis e oito dias depois. A contagem total de bactérias lácticas no intestino foi maior até o quarto dia de avaliação, no grupo alimentado com probióticos. A contagem de Vibrio spp. foi superior no grupo controle nos dias zero e dois. Até o quarto dia, a contagem total de hemócitos dos camarões após a inoculação de V. harveyi foi superior no grupo alimentado com probiótico em relação ao grupo controle. Não foi observada diferença na atividade da fenoloxidase. No sexto dia, após a substituição da dieta suplementada com probióticos por dieta controle, todos os parâmetros avaliados foram iguais nos dois grupos, o que indica que o tempo de atuação de L. plantarum em camarões é curto.Termos para indexação: Litopenaeus vannamei, Vibrio harveyi, contagem total de hemócitos, bactérias lácticas, fenoloxidase, probiótico.

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Section: Resultsmentioning

confidence: 99%

Time-related action of Lactobacillus plantarum in the bacterial microbiota of shrimp digestive tract and its action as immunostimulant

Vieira

Neto

Mouriño

et al. 2008

Pesq. agropec. bras.

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“…There have been several positive reports on the use of probiotics in shrimp culture, such as improved balance of the intestinal microbiota (Li et al, 2009), production/stimulation of the production of digestive enzymes , improved growth rate , feed efficiency (Lin et al, 2004), immunostimulation (Chiu et al, 2007), resistance to infection by bacterial (Chiu et al, 2007) and viral pathogens (Tseng et al, 2009). However, the use of probiotics is still controversial due to some negative results (no action on the host) (Meunpol et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

In vitro selection of bacteria with potential for use as probiotics in marine shrimp culture

Vinatea

2013

Pesq. agropec. bras.

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-The objective of this work was to isolate strains of lactic acid bacteria with probiotic potential from the digestive tract of marine shrimp (Litopenaeus vannamei), and to carry out in vitro selection based on multiple characters. The ideotype (ideal proposed strain) was defined by the highest averages for the traits maximum growth velocity, final count of viable cells, and inhibition halo against nine freshwater and marine pathogens, and by the lowest averages for the traits duplication time and resistance of strains to NaCl (1.5 and 3%), pH (6, 8, and 9), and biliary salts (5%). Mahalanobis distance (D 2 ) was estimated among the evaluated strains, and the best ones were those with the shortest distances to the ideotype. Ten bacterial strains were isolated and biochemically identified as Lactobacillus plantarum (3), L. brevis (3), Weissella confusa (2), Lactococcus lactis (1), and L. delbrueckii (1). Lactobacillus plantarum strains showed a wide spectrum of action and the largest inhibition halos against pathogens, both Gram-positive and negative, high growth rate, and tolerance to all evaluated parameters. In relation to ideotype, L. plantarum showed the lowest Mahalanobis (D 2 ) distance, followed by the strains of W. confusa, L. brevis, L. lactis, and L. delbrueckii. Among the analyzed bacterial strains, those of Lactobacillus plantarum have the greatest potential for use as a probiotic for marine shrimp.Index terms: Litopenaeus vannamei, acid lactic bacteria, bioprospection, pathogen inhibition. Seleção in vitro de bactérias com potencial para uso como probióticos na carcinicultura marinhaResumo -O objetivo deste trabalho foi isolar cepas de bactérias ácido-lácticas, com potencial probiótico do trato digestório de camarões marinhos e realizar seleção in vitro baseada em múltiplos caracteres. O ideótipo (cepa ideal proposta) foi definido por meio das maiores médias para os caracteres velocidade máxima de crescimento, contagem final de células viáveis e halo de inibição contra nove patógenos de origem continental e marinha, e pelas menores médias para os caracteres tempo de duplicação e tolerância das cepas a NaCl (1,5 e 3%), pH (6, 8 e 9) e sais biliares (5% Termos para indexação: Litopenaeus vannamei, bactérias ácido-lácticas, bioprospecção, inibição de patógenos.

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“…The bacterocin was produced by L. plantarum during the late logarithmic growth phase at a cell count of 1.5 x 10 9 cfu/mL. L. plantarum administration to the cultured aquatic animals showed increasing of immune response of the animal against bacterial pathogen (Messi et al, 2001;Chiu et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

THE EFFECT OF FEEDING Lactobacillus ON GROWTH, SURVIVAL RATE AND PROTEASE ACTIVITY OF Litopenaeus vannamei

Nafiqoh

Chang

Wang

2011

Indonesian Aquaculture Journal

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This study examined the effect of two Lactobacillus bacteria on protease activity and growth rate of Litopenaeus vannamei. An experiment was conducted to examine protease activity and growth rate. The experiment consisted of two treatment tanks, the first tank was provided with artemia immersed in 2.6 x 10 16 cfu/mL of bacteria solution, the second tank served as the control tank. After 20 days, the L. vannamei in the tank that received Lactobacillus have significantly different in growth, survival rate and protease activity (P<0.05) compared to the control, but no significant difference between Lactobacillus casei and Lactobacillus plantarum treatments. Within the digestive organ, protease activity of hepatopancreas and stomach demonstrated significant higher activity (P<0.05) compared to the intestine.

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Immune responses and gene expression in white shrimp, Litopenaeus vannamei, induced by Lactobacillus plantarum

Cited by 345 publications

References 49 publications

Time-related action of Lactobacillus plantarum in the bacterial microbiota of shrimp digestive tract and its action as immunostimulant

Time-related action of Lactobacillus plantarum in the bacterial microbiota of shrimp digestive tract and its action as immunostimulant

In vitro selection of bacteria with potential for use as probiotics in marine shrimp culture

THE EFFECT OF FEEDING Lactobacillus ON GROWTH, SURVIVAL RATE AND PROTEASE ACTIVITY OF Litopenaeus vannamei

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