Immune responses in Eimeria acervulina infected one-day-old broilers compared to amount of Eimeria in the duodenum, measured by real-time PCR

Swinkels, W.J.C.; Post, J.; Cornelissen, J.B.W.J.; Engel, B.; Boersma, W.J.A.; Rebel, Johanna M.J.

doi:10.1016/j.vetpar.2006.02.011

Cited by 27 publications

(17 citation statements)

References 26 publications

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“…During the first week, immune protection could be provided by maternal antibodies and innate effector (Bar-Shira and Friedman, 2006). However, the immune protection of maternal antibody in chicks was poor (Swinkels et al, 2006), so innate effector including goblet cell or MUC2 played an important role in intestinal immunity. Therefore, although green light improved intestinal immunity of the chicken embryo at E21 (hatch) in our study, it has an important biological role in intestinal immunity for the first week.…”

Section: Green Light Better Enhanced Development and Muc2 Expression mentioning

confidence: 99%

Effects of monochromatic light stimuli on the development and Muc2 expression of goblet cells in broiler small intestines during embryogenesis

Wang

Cao

et al. 2014

Poultry Science

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The effects of monochromatic light on the ontogeny, differentiation, and Muc2 expression level in goblet cells were studied in the small intestines of latestage broiler embryos. The embryos were exposed to blue light (B group), green light (G group), red light (R group), or darkness (D group) throughout the incubation period. On d 15 of incubation (E15), a few acidic goblet cells (only the sulfated subtype) were observed, and Muc2 mRNA expression was detected. On E18, however, neutral, acidic, and intermediate types, as well as the sulfated subtype, were observed in the small intestine, and a decreasing gradient of goblet cell density was found along the duodenum to ileum axis. Up to E21, 3 types of goblet cells and 3 acidic cell subtypes were found in all the small intestines. The goblet cell density increased along the duodenum to ileum axis. Monochromatic light stimulation resulted in no significant differences in the density and types of goblet cells between the different treatment groups on E15 and E18, but an increased Muc2 mRNA expression level was detected on E18 in the G group compared with the other treatment groups. On E21, the goblet cell density, proportion of acidic goblet cells, and Muc2 mRNA expression level increased in the G group compared with other treatment groups. These results suggest that the ontogeny and differentiation of goblet cells in broiler embryos display temporal and spatial differences. Green monochromatic light may have the potential to promote the proliferation and maturation of as well as the expression of Muc2 mRNA in goblet cells of broiler embryos.

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Section: Green Light Better Enhanced Development and Muc2 Expression mentioning

confidence: 99%

Effects of monochromatic light stimuli on the development and Muc2 expression of goblet cells in broiler small intestines during embryogenesis

Wang

Cao

et al. 2014

Poultry Science

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“…While sublethal injury by pressure has been detected in bacteria (Mañas & Pagán, 2005), this phenomenon has not been documented in parasites. The severity of coccidiosis symptoms can be correlated with dosage, and while this can vary with the isolate, ingestion of as few as 500 oocysts of E. acervulina has been demonstrated to cause weight depression and lesions (Swinkels et al, 2006). Therefore, if viable oocysts remained after pressure treatment in this study, the surviving population was likely to be fewer than 500 oocysts.…”

Section: Discussionmentioning

confidence: 79%

Effects of high hydrostatic pressure on Eimeria acervulina pathogenicity, immunogenicity and structural integrity

Shearer

Wilkins

Jenkins

et al. 2007

Innovative Food Science & Emerging Technologies

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“…A number of previous studies (Blake et al, 2006(Blake et al, , 2008Swinkels et al, 2006Swinkels et al, , 2007 have developed assays for quantification of Eimeria spp., but they lack the crossspecies breadth of the real-time PCR HRM curve analysis described here. Further studies are necessary to fully investigate the capacity of the real-time PCR HRM curve analysis for quantification of Eimeria spp.…”

Section: Discussionmentioning

confidence: 99%

A polymerase chain reaction-coupled high-resolution melting curve analytical approach for the monitoring of monospecificity of avianEimeriaspecies

Kirkpatrick

Blacker

Woods³

et al. 2009

Avian Pathology

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Coccidiosis is a significant disease of poultry caused by different species of Eimeria. Differentiation of Eimeria species is important for the quality control of the live attenuated Eimeria vaccines derived from monospecific lines of Eimeria spp. In this study, high-resolution melting (HRM) curve analysis of the amplicons generated from the second internal transcribed spacer of nuclear ribosomal DNA (ITS-2) was used to distinguish between seven pathogenic Eimeria species of chickens, and the results were compared with those obtained from the previously described technique, capillary electrophoresis. Using a series of known monospecific lines of Eimeria species, HRM curve analysis was shown to distinguish between Eimeria acervulina, Eimeria brunetti, Eimeria maxima, Eimeria mitis, Eimeria necatrix, Eimeria praecox and Eimeria tenella. Computerized analysis of the HRM curves and capillary electrophoresis profiles could detect the dominant species in several specimens containing different ratios of E. necatrix and E. maxima and of E. tenella and E. acervulina. The HRM curve analysis identified all of the mixtures as "variation" to the reference species, and also identified the minor species in some mixtures. Computerized HRM curve analysis also detected impurities in 21 possible different combinations of the seven Eimeria species. The PCR-based HRM curve analysis of the ITS-2 provides a powerful tool for the detection and identification of pure Eimeria species. The HRM curve analysis could also be used as a rapid tool in the quality assurance of Eimeria vaccine production to confirm the purity of the monospecific cell lines. The HRM curve analysis is rapid and reliable and can be performed in a single test tube in less than 3 h.

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Immune responses in Eimeria acervulina infected one-day-old broilers compared to amount of Eimeria in the duodenum, measured by real-time PCR

Cited by 27 publications

References 26 publications

Effects of monochromatic light stimuli on the development and Muc2 expression of goblet cells in broiler small intestines during embryogenesis

Effects of monochromatic light stimuli on the development and Muc2 expression of goblet cells in broiler small intestines during embryogenesis

Effects of high hydrostatic pressure on Eimeria acervulina pathogenicity, immunogenicity and structural integrity

A polymerase chain reaction-coupled high-resolution melting curve analytical approach for the monitoring of monospecificity of avianEimeriaspecies

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