Immune responses in Indonesian thin tail and Merino sheep during a primary infection with Fasciola gigantica: lack of a specific IgG2 antibody response is associated with increased resistance to infection in Indonesian sheep

Hansen, Diana S.; Clery, Daniel; Estuningsih, Sri; Widjajanti, Sri; Partoutomo, Sutijono; Spithill, Terry W.

doi:10.1016/s0020-7519(99)00038-7

Cited by 37 publications

(19 citation statements)

References 40 publications

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“…Indeed, the experimental data obtained in vivo support this hypothesis. ITT sheep exhibit a rapid induction of eosinophilia and immunoglobulins G and E within 8 to 14 days of infection with F. gigantica (9), and significant killing of the invading parasites in ITT sheep occurs within 2 to 4 weeks of infection and before significant damage to the liver occurs (38,39,44). This lack of damage to the liver observed within 2 to 4 weeks of infection suggests that many invading parasites are killed in the perito- Interestingly, rats mediate effective immunity to F. hepatica and also have resident cells (monocytes/macrophages) which can kill parasites in vitro in the presence of a parasite-specific antibody, albeit the effector mechanism is nitric oxide and not superoxide radicals (33,34,41,46,47).…”

Section: Discussionmentioning

confidence: 99%

Peritoneal Lavage Cells of Indonesian Thin-Tail Sheep Mediate Antibody-Dependent Superoxide Radical Cytotoxicity In Vitro against Newly Excysted Juvenile Fasciola gigantica but Not Juvenile Fasciola hepatica

et al. 2007

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Indonesian thin-tail (ITT) sheep resist infection byFasciola gigantica by an immunological mechanism within 2 to 4 weeks of infection yet are susceptible to F. hepatica infection. Studies of ITT sheep show that little liver damage occurs following F. gigantica infection, suggesting that the invading parasites are killed within the peritoneum or shortly after reaching the liver. We investigated whether cells isolated from the peritoneums of ITT sheep could kill newly excysted juvenile F. gigantica in vitro and act as a potential mechanism of resistance against F. gigantica infection. Peritoneal cells from F. gigantica-infected sheep, rich in macrophages and eosinophils, mediated antibody-dependent cytotoxicity against juvenile F. gigantica in vitro. Cytotoxicity was dependent on contact between the parasite and effector cells. Isolated mammary gland eosinophils of F. gigantica-infected sheep, or resident peritoneal monocytes/macrophages from uninfected sheep, also killed the juvenile parasites in vitro. By using inhibitors, we show that the molecular mechanism of killing in these assays was dependent on the production of superoxide radicals by macrophages and eosinophils. In contrast, this cytotoxic mechanism was ineffective against juvenile F. hepatica parasites in vitro. Analysis of superoxide dismutase activity and mRNA levels showed that activity and gene expression were higher in F. hepatica than in F. gigantica, suggesting a possible role for this enzyme in the resistance of F. hepatica to superoxide-mediated killing. We suggest that ovine macrophages and eosinophils, acting in concert with a specific antibody, may be important effector cells involved in the resistance of ITT sheep to F. gigantica.

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Section: Discussionmentioning

confidence: 99%

Peritoneal Lavage Cells of Indonesian Thin-Tail Sheep Mediate Antibody-Dependent Superoxide Radical Cytotoxicity In Vitro against Newly Excysted Juvenile Fasciola gigantica but Not Juvenile Fasciola hepatica

et al. 2007

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“…It is caused by the liver flukes, Fasciola hepatica, in temperate zones and Fasciola gigantica in tropical and subtropical regions. The infection has been studied in cattle (Bossaert et al, 2000) and rat (Poitou et al, 1993;Paz et al, 1998), which exhibit partial resistance, as well as sheep (Hansen et al, 1999) and goat (Martinez-Moreno et al, 1999), which display nonprotective immunity to a challenge infection. Among sheep breeds, there are examples of acquired resistance in the Indonesian thin-tailed and susceptibility in the Merino.…”

Section: Introductionmentioning

confidence: 99%

“…The effector cells, eosinophils, neutrophils and macrophages, following attachment and degranulation, induce nitric oxide-mediated killing of the parasite (Spithill et al, 1997). The absence of resistance has been attributed to the following evasion mechanisms: (1) formation of specific antibody-glycocalyx complexes and shedding of the glycocalyx to prevent attachment of inflammatory effector cells (Glauert et al, 1985); (2) generation of parasite-specific IgG2 blocking antibodies in susceptible sheep breeds to interfere with IgG1-dependent macrophagemediated cytotoxicity (Hansen et al, 1999); and (3) down-regulation of an early T helper 1 cytokine-regulated protection by a later T helper 2 cytokine-controlled nonprotective mechanism that favours chronic infection (Hansen et al, 1999). The latter mechanism results from the inhibition of gamma interferon-activated effector cells of the monocyte/macrophage lineage by interleukin-10.…”

Section: Introductionmentioning

confidence: 99%

The Liver Flukes Fasciola gigantica and Fasciola hepatica Express the Leucocyte Cluster of Differentiation Marker CD77 (Globotriaosylceramide) in Their Tegument

Wuhrer¹,

Berkefeld²,

Dennis³

et al. 2001

Biological Chemistry

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Glycosphingolipids from the parasitic liver flukes Fasciola gigantica and Fasciola hepatica were isolated and their carbohydrate moieties were structurally analysed by methylation analysis, exoglycosidase treatment, on-target exoglycosidase cleavage and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. For both liver fluke species, the ceramide monohexosides Gal1-ceramide and Glc1-ceramide were found in relative amounts of 1.0 to 0.1, respectively. From F. gigantica, the ceramide dihexoside was isolated in sufficient amounts to be structurally determined as lactosylceramide, Gal beta4-Glc1-ceramide, while for both liver fluke species the ceramide trihexoside was shown to be Gal alpha4Gal beta4-Glc1-ceramide, which is designated as either globotriaosylceramide, Pk-blood group antigen or CD77 leucocyte cluster of differentiation antigen. To our knowledge, this is the first report on the expression of globo-series glycosphingolipids in non-mammalian species. Ceramide analysis of ceramide monohexosides yielded as major components octadecanoic and 2-hydroxyoctadecanoic fatty acids together with C18- and C20-phytosphingosines. By the use of an anti-CD77 monoclonal antibody and the Escherichia coli Shiga toxin B1 subunit, globotriaosylceramide could be immunolocalised to the tegument of F. hepatica cryosections. The sharing of CD77 between liver flukes and their mammalian hosts fits in with the concept of molecular mimicry, which is closely parallel to the established imitation of host CD15 (Lewis X) displayed by the blood fluke Schistosoma mansoni.

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“…FABP and GST immunization caused a rapid proliferation of the eosinophils in these experimental calves. An increase in the eosinophil count in F. gigantica-infected buffaloes (15.2%) and in sheep experimentally infected with F. gigantica was reported by Zhang et al (2006), Hansen et al (1999) and Mulcahy et al (1999). Eosinophils play a crucial role in inducing resistance during parasitic infections (Butterworth 1984).…”

Section: Challenge-infect Contmentioning

confidence: 99%

Vaccination of buffaloes with Fasciola gigantica recombinant glutathione S-transferase and fatty acid binding protein

et al. 2011

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Fasciola gigantica, causative agent of tropical fasciolosis, inflicts substantial economic losses on the livestock industry, affecting severely buffalo productivity in the tropical countries. Very few vaccination trials with different target antigens against F. gigantica infection have been conducted in this host. Present study describes a vaccination trial in buffaloes with F. gigantica recombinant glutathione S-transferase and fatty acid binding protein. The two recombinant proteins were expressed in Escherichia coli and evaluated for their immunoprophylactic potential in buffalo calves, using montanide 70 M-VG, a mineral oil-based adjuvant, for delivering the antigens. Buffalo calves were distributed in three groups, with group I, II and III calves immunized with recombinant glutathione S-transferase, fatty acid binding protein and a cocktail of these two antigens, respectively. Immunization of the calves evoked a mixed IgG1 and IgG2 antibody response. Present vaccination trial in these animals achieved a maximum protection level of 35%, when the two antigens were used in combination. Eosinophils were measured in both immunized and non-immunized challenge control animals, which showed a steady increase in their count in response to immunization with both the antigens and infection with F. gigantica, respectively.

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Immune responses in Indonesian thin tail and Merino sheep during a primary infection with Fasciola gigantica: lack of a specific IgG2 antibody response is associated with increased resistance to infection in Indonesian sheep

Cited by 37 publications

References 40 publications

Peritoneal Lavage Cells of Indonesian Thin-Tail Sheep Mediate Antibody-Dependent Superoxide Radical Cytotoxicity In Vitro against Newly Excysted Juvenile Fasciola gigantica but Not Juvenile Fasciola hepatica

Peritoneal Lavage Cells of Indonesian Thin-Tail Sheep Mediate Antibody-Dependent Superoxide Radical Cytotoxicity In Vitro against Newly Excysted Juvenile Fasciola gigantica but Not Juvenile Fasciola hepatica

The Liver Flukes Fasciola gigantica and Fasciola hepatica Express the Leucocyte Cluster of Differentiation Marker CD77 (Globotriaosylceramide) in Their Tegument

Vaccination of buffaloes with Fasciola gigantica recombinant glutathione S-transferase and fatty acid binding protein

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