Immune responses in pigs and cattle vaccinated with half-volume foot-and-mouth disease vaccine

Park, Min-Eun; You, Su-Hwa; Lee, Seo-Yong; Lee, Kwang‐Nyeong; Ko, Mi-Kyeong; Choi, Joo-Hyung; Lee, Jong-Soo

doi:10.4142/jvs.2017.18.s1.323

Cited by 13 publications

(9 citation statements)

References 16 publications

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“…Thus, it is desirable to use vaccines that can protect against at least 2 different topotype strains in Korea. Previously, a vaccine was developed using the virus responsible for the SEA topotype outbreak in November 2010 in Korea; however, that vaccine showed low immunity from the virus that caused the 2014 outbreak [7].…”

Section: Discussionmentioning

confidence: 99%

“…However, although these vaccine strains provided protection, some vaccine strains belonging to different topotypes induced insufficient antibody reactions after immunization or induced delayed immunity. Previously, an FMD vaccine was developed using the virus that caused the 2010 (November) outbreak in Korea; this vaccine was evaluated for its immune response against the virus strain that caused a recent outbreak, and it was observed that it has a different immune reactivity, one that induced almost no production of antibodies [7]. Therefore, this result suggested that the same SEA topotype vaccine strains do not always guarantee protection.…”

Section: Introductionmentioning

confidence: 99%

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New foot-and-mouth disease vaccine, O JC-R, induce complete protection to pigs against SEA topotype viruses occurred in South Korea, 2014–2015

Choi

et al. 2019

J Vet Sci

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Foot-and-mouth disease (FMD) is an acute epidemic that spreads rapidly among cattle and pigs. In 2014, in Korea, despite enforced vaccination, the type O Southeast Asia (SEA) topotype viruses (Mya-98 lineage) infected mainly cattle and pigs simultaneously, thereby causing enormous damage. If a vaccine that is completely protective against this FMD virus is developed and used, it can become a very important preventive measure in Asia, which is where this type of virus mainly circulates. The SEA topotype has been steadily evolving and transforming into new variations since it became epidemic in Asia. Therefore, it became necessary to develop a new vaccine that could provide protection against the FMD virus strain that was responsible for the 2014–2015 outbreak in Korea. This study aimed to develop a vaccine that would provide complete protection against the SEA topotype FMD virus to control sporadic FMD outbreaks, which occur despite the enforcement of vaccination, and to completely prevent virus shedding, thereby preventing the virus from spreading. The vaccine candidate virus developed in this study showed low pathogenicity and can be distinguished from the wild-type FMD virus strain. The developed vaccine was able to protect mice from SEA and Middle East–South Asia topotype virus strains and induced high titers of antibodies against both virus strains in pigs, thereby confirming the sufficiency of its protective function. In particular, the results of the SEA topotype virus challenge test in pigs revealed that perfect immunity was created in the vaccinated pigs, without virus shedding and viremia.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

New foot-and-mouth disease vaccine, O JC-R, induce complete protection to pigs against SEA topotype viruses occurred in South Korea, 2014–2015

Choi

et al. 2019

J Vet Sci

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“…To measure the immune response and oil residue in the vaccination site, the O TWN antigen (15 µg/mL, 1 dose), 10% Al(OH) 3 , and saponin (0.5 mg/mL/dose) were mixed with each adjuvant (ISA15, ISA 206) [ 13 ]. A 1/10 dose of vaccine was administered to C57BL/6 7-week-old mice (5 mice/group) vis the intramuscular (IM), subcutaneous (SC) inoculation, and the fatty tissue (inguinal fat) route.…”

Section: Methodsmentioning

confidence: 99%

Improved immune responses and safety of foot-and-mouth disease vaccine containing immunostimulating components in pigs

Choi

You

et al. 2020

J Vet Sci

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Background: The quality of a vaccine depends strongly on the effects of the adjuvants applied simultaneously with the antigen in the vaccine. The adjuvants enhance the protective effect of the vaccine against a viral challenge. Conversely, oil-type adjuvants leave oil residue inside the bodies of the injected animals that can produce a local reaction in the muscle. The longterm immunogenicity of mice after vaccination was examined. ISA206 or ISA15 oil adjuvants maintained the best immunity, protective capability, and safety among the oil adjuvants in the experimental group. Objectives: This study screened the adjuvant composites aimed at enhancing foot-andmouth disease (FMD) immunity. The C-type lectin or toll-like receptor (TLR) agonist showed the most improved protection rate. Methods: Experimental vaccines were fabricated by mixing various known oil adjuvants and composites that can act as immunogenic adjuvants (gel, saponin, and other components) and examined the enhancement effect on the vaccine. Results: The water in oil (W/O) and water in oil in water (W/O/W) adjuvants showed better immune effects than the oil in water (O/W) adjuvants, which have a small volume of oil component. The W/O type left the largest amount of oil residue, followed by W/O/W and O/W types. In the mouse model, intramuscular inoculation showed a better protection rate than subcutaneous inoculation. Moreover, the protective effect was particularly weak in the case of inoculation in fatty tissue. The initial immune reaction and persistence of long-term immunity were also confirmed in an immune reaction on pigs. Conclusions: The new experimental vaccine with immunostimulants produces improved immune responses and safety in pigs than general oil-adjuvanted vaccines.

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“…The presence of FMDV particles in a sample of each fraction was determined using a lateral flow device (BioSign FMDV Ag; Princeton BioMeditech, USA). Before its use in the animal experiment, innocuity of the pre-PEG treatment supernatant was tested through a BHK-21 cell line and LF-BK cell line at least twice to check that no cytopathic effect (CPE) had occurred, thereby confirming the absence of live virus in the supernatant [19]. The 146S virus particles were quantified using the spectrophotometer at a wavelength of 259 nm.…”

Section: Methodsmentioning

confidence: 99%

Needleless intradermal vaccination for foot-and-mouth disease induced granuloma-free effective protection in pigs

et al. 2019

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Vaccination is one of the most effective ways of controlling and preventing foot-and-mouth disease (FMD) outbreaks. The effective prevention of this disease requires the use of high-quality vaccines to meet the criteria that enable customers to use them simply. The administration of FMD vaccines containing oil-based adjuvants in pigs can induce the formation of granuloma in the muscle of the vaccinated, which makes these vaccines a less preferable option. Therefore, it is important to establish an FMD vaccine and vaccine delivery tool that offers better immunity and safer application. This study compared the immune responses of intramuscular and needleless intradermal vaccination in pigs. When the same amount of an FMD virus (FMDV) antigen was administered to pigs, both the intradermally and intramuscularly vaccinated groups were protected completely against a challenge of the homologous FMDV, but the intramuscularly vaccinated group showed an overall higher level of neutralizing antibodies. Importantly, the formation of granuloma in muscle could be excluded in the intradermally vaccinated group. Of the oil-based adjuvants selected in this study, ISA 207 was effective in eliciting immunogenicity in intradermal vaccination. In conclusion, a new vaccine formula can be chosen for the delivery of intradermal route to exclude the possibility of local reactions in the muscle and generate protective immunity against an FMDV challenge.

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Immune responses in pigs and cattle vaccinated with half-volume foot-and-mouth disease vaccine

Cited by 13 publications

References 16 publications

New foot-and-mouth disease vaccine, O JC-R, induce complete protection to pigs against SEA topotype viruses occurred in South Korea, 2014–2015

New foot-and-mouth disease vaccine, O JC-R, induce complete protection to pigs against SEA topotype viruses occurred in South Korea, 2014–2015

Improved immune responses and safety of foot-and-mouth disease vaccine containing immunostimulating components in pigs

Needleless intradermal vaccination for foot-and-mouth disease induced granuloma-free effective protection in pigs

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