Immune responses to vaccines delivered by encapsulation into and/or adsorption onto cationic lipid-PLGA hybrid nanoparticles

Liu, Lanxia; Ma, Pingchuan; Hai, Wang; Zhang, Chao; Sun, Hongfan; Wang, Chun; Song, Cunxian; Leng, Xigang; Kong, Deling; Ma, Guilei

doi:10.1016/j.jconrel.2016.01.050

Cited by 93 publications

(72 citation statements)

References 32 publications

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“…Although uptake inhibition data suggest a common uptake mechanism for soluble OVA and OVA PNPs, the attenuated acidification of PNPs as compared to soluble OVA suggests different internal routing for PNP and soluble antigen. Our data is also consistent with lysosomal colocalization studies that show antigen in nanoparticle cores can achieve cytosolic delivery, while soluble or nanoparticle-adsorbed antigen cannot (18). The pH 4.7 experienced by soluble OVA is characteristic of endosomal fusion with lysosomes, a process promoting proteolytic antigen degradation (48).…”

Section: Discussionsupporting

confidence: 90%

“…Biodegradable polymers such as chitosan and poly lactic-co-glycolic acid (PLGA) have been studied extensively for nanoparticle vaccine encapsulation(15). Incorporation of antigens in a nanoparticulate polymer matrix has shown that controlled release of antigen to the immune system gives an advantage over vaccination with soluble antigen(16-18). However, recently surface-based display of antigen on nanoparticles, such as on virus-like particles(16) or protein particles(19) has gained increasing attention.…”

Section: Introductionmentioning

confidence: 99%

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Effects of ovalbumin protein nanoparticle vaccine size and coating on dendritic cell processing

et al. 2017

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Nanoparticle vaccine delivery platforms are a promising technology for enhancing vaccine immunogenicity. Protein nanoparticles (PNPs), made entirely from antigen, have been shown to induce protective immune responses against influenza. However, the fundamental mechanisms by which PNPs enhance component protein immunogenicity are not understood. Here, we investigate the role of size and coating of model ovalbumin (OVA) PNPs on particle uptake and trafficking, as well as on inflammation and maturation factor expression in dendritic cells (DCs) in vitro. OVA PNPs enhance antigen uptake in a size-independent manner, and experience attenuated endosomal acidification as compared to soluble OVA. OVA PNPs also trigger Fc receptor upregulation. Expression of cytokines IL-1β and TNF-α were PNP size- and coating-dependent, with small (~270 nm) nanoparticles triggering greater inflammatory cytokine production than large (~560 nm) particles. IL-1β expression by DCs in response to PNP stimulation implies activation of the inflammasome, a pathway known to be activated by certain types of nanoparticulate adjuvants. The attenuated acidification and pro-inflammatory profile generated by PNPs in DCs demonstrate that physical biomaterial properties can modulate dendritic cell-mediated antigen processing and adjuvancy. In addition to nanoparticles’ enhancement of DC antigen uptake, our work suggests that vaccine nanoparticle size and coating are uptake-independent modulators of immunogenicity.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Effects of ovalbumin protein nanoparticle vaccine size and coating on dendritic cell processing

et al. 2017

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“…However, Ags adsorbed on a carrier surface may be released at a relatively high rate, leaving a limited time window for APC recruitment, and this may cause inefficient and unsynchronized cellular uptake of Ags and adjuvants to form a population of APCs that are Ag-positive but adjuvant-negative and unable to properly present Ags, as mentioned above [103]. Recent studies also reported that whilst encapsulation of Ags within a carrier engendered more efficient lysosomal escape and cross-presentation of Ags in DCs than did absorption on a carrier [117], whereas the combination of Ag encapsulation and absorption of Ags with PLGA NPs induced more potent anti-Ag immunoresponses than each mode alone did, due, perhaps, to both adequate initial and persistent Ag exposure [118].…”

Section: Ag-loading Modementioning

confidence: 99%

Liposomes used as a vaccine adjuvant-delivery system: From basics to clinical immunization

Wang

Chen

Wang

2019

Journal of Controlled Release

226

155

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“…In the cellular uptake process, exogenous particles would be transported to endosomes initially and lysosomes eventually after being internalized into the cells . We detected the intracellular location of OVA‐Cu‐HVs in cells by staining with LAMP1 to identify lysosomes.…”

Section: Resultsmentioning

confidence: 99%

Antigen‐Inorganic Hybrid Flowers‐Based Vaccines with Enhanced Room Temperature Stability and Effective Anticancer Immunity

Liu

Zhu

Lü

et al. 2019

Adv Healthcare Materials

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Particle‐based antigen carriers as adjuvants play an important role in vaccine development. Herein, an antigen‐inorganic hybrid flower‐like particle is developed as a novel vaccine carrier. Model antigen ovalbumin (OVA)‐copper (II) sulfate hybrid vaccines (OVA‐Cu‐HVs) are mildly and facilely constructed through a biomimetic mineralization process. OVA‐Cu‐HVs facilitate cellular uptake in antigen‐presenting cells and the internalization of OVA‐Cu‐HVs involves macropinocytosis‐mediated endocytosis. OVA‐Cu‐HVs can release OVA in a pH‐responsive behavior and promote cytosolic release of antigen to enhance antigen cross‐presentation. Immunization with OVA‐Cu‐HVs promotes the maturation of dendritic cells in draining lymph nodes, induces robust antigen‐specific T lymphocyte response, and inhibits tumor growth in vivo. In addition, OVA‐Cu‐HVs are efficacious after being stored for 4 weeks at room temperature and are expected to simplify vaccine storage and lower the cost of cold storage for transportation. Looking forward, OVA‐Cu‐HVs may hold strong potential to be as an effective vaccine delivery platform, which will facilitate the application of organic–inorganic hybrid flowers in biomedical areas.

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Immune responses to vaccines delivered by encapsulation into and/or adsorption onto cationic lipid-PLGA hybrid nanoparticles

Cited by 93 publications

References 32 publications

Effects of ovalbumin protein nanoparticle vaccine size and coating on dendritic cell processing

Effects of ovalbumin protein nanoparticle vaccine size and coating on dendritic cell processing

Liposomes used as a vaccine adjuvant-delivery system: From basics to clinical immunization

Antigen‐Inorganic Hybrid Flowers‐Based Vaccines with Enhanced Room Temperature Stability and Effective Anticancer Immunity

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