Immune-specific production of gamma interferon in human lymphocyte cultures in response to mumps virus

Nakayama, Tetsuo

doi:10.1128/iai.40.2.486-492.1983

Cited by 14 publications

(3 citation statements)

References 16 publications

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“…Interferon gamma is crucial for the function of both innate and adaptive immune responses e.g. due to its stimulatory function on macrophages [ 54 – 56 ]. Indeed, we observed both types of immune response to be affected by the RAO condition, and therefore the decrease in IFNG in RAO horses could be involved in the RAO pathomechanism ( Fig 6 ).…”

Section: Discussionmentioning

confidence: 99%

Impaired Cell Cycle Regulation in a Natural Equine Model of Asthma

et al. 2015

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Recurrent airway obstruction (RAO) is a common and potentially debilitating lower airway disease in horses, which shares many similarities with human asthma. In susceptible horses RAO exacerbation is caused by environmental allergens and irritants present in hay dust. The objective of this study was the identification of genes and pathways involved in the pathology of RAO by global transcriptome analyses in stimulated peripheral blood mononuclear cells (PBMCs). We performed RNA-seq on PBMCs derived from 40 RAO affected and 45 control horses belonging to three cohorts of Warmblood horses: two half-sib families and one group of unrelated horses. PBMCs were stimulated with hay dust extract, lipopolysaccharides, a recombinant parasite antigen, or left unstimulated. The total dataset consisted of 561 individual samples. We detected significant differences in the expression profiles between RAO and control horses. Differential expression (DE) was most marked upon stimulation with hay dust extract. An important novel finding was a strong upregulation of CXCL13 together with many genes involved in cell cycle regulation in stimulated samples from RAO affected horses, in addition to changes in the expression of several HIF-1 transcription factor target genes. The RAO condition alters systemic changes observed as differential expression profiles of PBMCs. Those changes also depended on the cohort and stimulation of the samples and were dominated by genes involved in immune cell trafficking, development, and cell cycle regulation. Our findings indicate an important role of CXCL13, likely macrophage or Th17 derived, and the cell cycle regulator CDC20 in the immune response in RAO.

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Section: Discussionmentioning

confidence: 99%

Impaired Cell Cycle Regulation in a Natural Equine Model of Asthma

et al. 2015

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“…There is an immune-specific production of the lymphokine gamma interferon (IFN-y) when mononuclear cells are stimulated by a specific antigen. For example, mononuclear cells from seropositive donors produce IFN-y in response to viral antigens of herpes simplex virus (8,9,13,15), Epstein-Barr virus (1), cytomegalovirus (CMV) (14), vaccinia virus (6), mumps virus (11), and influenza virus (5).…”

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confidence: 99%

“…Previous studies have used a bioassay for IFN-y; however, there is marked variability both in the time of earliest detection of IFN-y and in the day of maximum production (1,8,11). Bioassays quantitate IFN-y on the basis of inhibition of viral growth in cultured cells.…”

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confidence: 99%