Immune stimulatory and anti-HIV-1 potential of extracts derived from marine brown algae <i>Padina tetrastromatica</i>

Suram, Dinesh; Hanna, Luke Elizabeth; Maheshkumar, K; Karuppiah, Ponmurugan; Al-Dhabi, Naïf Abdullah; Murugan, Pitchai

doi:10.1515/jcim-2019-0071

Cited by 9 publications

(5 citation statements)

References 35 publications

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“…Perhaps this decrease might also be caused by the instability in the cell culture medium, which was replaced after every collection of the supernatant. Similar results were reported by Subramaniam et al (2020) using a different endophytic fungus on PBMCs. Methanolic extracts (100 μg/ml) of Padina tetrastromatica inhibited HIV-1 by more than 50% and showed a significant reduction in HIV-1 p24 levels after 7 days of incubation.…”

Section: Discussionsupporting

confidence: 90%

Secondary metabolites produced by endophytic fungi, Alternaria alternata, as potential inhibitors of the human immunodeficiency virus

et al. 2022

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Antiretroviral treatment has significantly reduced human immunodeficiency virus infection and mortality. However, the current treatment regimen is limited by adverse side effects, the emergence of drug resistance, and the inability to eliminate viral reservoirs. Here, fifteen endophytic fungi were isolated from Sclerocarya birrea and Hypoxis plants. Crude extracts of Alternaria alternata (strain ID PO4PR1, PO4PR2, and PO2PL1) of the fifteen isolate’s crude extracts showed anti-HIV-1 activity in TZM-bl cell line at inhibitory concentration (IC50) values ranging from 0.017 to 1.170 μg/ml. The three crude extracts also maintained the virus replication inhibition profile on PBMCs and CD4+ T cells at concentrations ranging from 0.3 to 50.2 ng/ml. Partial purification using the solid phase extraction and analysis with Gas Chromatography-Mass spectrophotometry showed a diverse profile. The bioactive compounds were identified based on peak area, retention time, similarity index. The major compounds from GC-MS analysis of A. Alternata revealed the existence of cyclotrisiloxane octamethyl (22.92%); Propaninitrile (16,67%); Pyrrolol[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methyl propyl) (10.42%); Silane, diethylethoxy(2-ethoxyethyloxy) (4.17%); Coumarin, 3,4-dihydro-4,5,7-trimethyl- 4,5,7-Trimethyl-2-chromanone (13.7%) and 1,2-Cyclobutanedicarbonitrile (2.08%) with previously reported biological activities such as antimicrobial, anti-inflammatory and antioxidant properties. Therefore, these bioactive compounds from A. alternata fungal endophytes could be repurposed as potential anti-HIV agents. This study showed the potential of endophytic fungi, Alternaria alternata from S. birrea, and Hypoxis species as producers of anti-HIV compounds.

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Section: Discussionsupporting

confidence: 90%

Secondary metabolites produced by endophytic fungi, Alternaria alternata, as potential inhibitors of the human immunodeficiency virus

et al. 2022

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“…The recorded IC 50 of seaweed extracts on WI-38 cell line was above the lower concentrations (10, 50, 100, 200 and 400 µg/mL), which supports the extracts safety compared to the Cisplatin (chemotherapeutic drug) (Table 3 and 4). This action is in agreement with the observation that no toxicity was detected in all tested concentrations (0-400 μg/mL) of the methanolic and aqueous extract of Padina tetrastromatica on Peripheral blood mononuclear cells (PBMCs) after 48 hours' treatment (Subramaniam et al, 2020). Jania Rubens exhibited a non-effective cytotoxic activity on normal human HCEC (Human Corneal Epithelial Cells) cell line after 72 hours at different concentrations (0-200 μg/mL) (Alghazeer et al, 2016).…”

Section: In Vitro Cytotoxicity Of the Four Seaweed Extracts On Normal Cell Line (Wi-38 Human Caucasian Fibroblast-like Fetal Lung Cell LIsupporting

confidence: 88%

Antioxidant, antidiabetic, anti-inflammatory and anticancer potential of some seaweed extracts

et al. 2022

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This study aims to evaluate the secondary metabolites, cytotoxicity, antioxidant, antidiabetic, anti-inflammatory, and anticancer activities of four selected seaweeds Padina pavonica, Taonia atomaria, Jania rubens, and Corallina elongata. The maximum value of phenolic content (176.7 ± 6.9 mg gallic acid equivalents/g crude extract) and tannin content (26.5 ± 4.3 mg tannic acid equivalents/g crude extract), was shown in the methanol extract of J. rubens and P. pavonica, respectively. While the ethanol extract of T. atomaria recorded the highest values of total flavonoid content (374.1 ±27.4 mg quercetin equivalents/g crude extract) and total saponins content (30.2 ± 0.7 mg cholesterol equivalents/g crude extract, respectively) compared to other seaweeds. Methanol extract of J. rubens and C. elongata exhibited the greatest content of alkaloids (25.8 ± 4.4 and 22.7 ± 2.6 mg/g d wt.). The methanol extract of P. pavonica established the highest DPPH radical scavenging activity (55.7% ± 0.1) at 50 μg/mL. Among the selected seaweed extracts, the ethanol extract of T. atomaria demonstrated the maximum α-amylase inhibition capacity (66.3% ± 0.0). Methanol extract of J. rubens and P. pavonica species effectively prevented the hypotonicity-induced haemolysis in a concentration-dependent manner compared with the diclofenac potassium as a standard anti-inflammatory drug. In vitro, the reported results also supported the safety and non-toxicity of the four seaweed extracts on WI-38 cell line at lower concentrations. J. rubens and P. pavonica methanol extracts recorded the highest cell growth inhibition of the HeLa Cancer Cell Lines compared to other seaweed extracts and the standard drug Cisplatin.

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“…Our results are also consistent with the finding that, after 48 h of treatment, no toxicity was seen in any of the concentrations (0-400 g/mL) of the methanolic and aqueous extract of Padina spp. on peripheral blood mononuclear cells (PBMCs) [72]. According to the results, the P. pavonica extract has antibacterial properties against all the bacteria tested.…”

Section: Cytotoxicity Effect Of Padina Pavonica Extractmentioning

confidence: 99%

Utilization of Algae Extracts as Natural Antibacterial and Antioxidants for Controlling Foodborne Bacteria in Meat Products

Hamad,

Samy,

Mehany

et al. 2023

Foods

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Padina pavonica, Hormophysa cuneiformis, and Corallina officinalis are three types of algae that are assumed to be used as antibacterial agents. Our study’s goal was to look into algal extracts’ potential to be used as food preservative agents and to evaluate their ability to inhibit pathogenic bacteria in several meat products (pastirma, beef burger, luncheon, minced meat, and kofta) from the local markets in Alexandria, Egypt. By testing their antibacterial activity, results demonstrated that Padina pavonica showed the highest antibacterial activity towards Bacillus cereus, Staphylococcus aureus, Escherichia coli, Streptococcus pyogenes, Salmonella spp., and Klebsiella pneumoniae. Padina pavonica extract also possesses most phenolic and flavonoid content overall. It has 24 mg gallic acid equivalent/g and 7.04 mg catechol equivalent/g, respectively. Moreover, the algae extracts were tested for their antioxidant activity, and the findings were measured using ascorbic acid as a benchmark. The IC50 of ascorbic acid was found to be 25.09 μg/mL, while Padina pavonica exhibited an IC50 value of 267.49 μg/mL, Corallina officinalis 305.01 μg/mL, and Hormophysa cuneiformis 325.23 μg/mL. In this study, Padina pavonica extract was utilized in three different concentrations (Treatment 1 g/100 g, Treatment 2 g/100 g, and Treatment 3 g/100 g) on beef burger as a model. The results showed that as the concentration of the extract increased, the bacterial inhibition increased over time. Bacillus cereus was found to be the most susceptible to the extract, while Streptococcus pyogenes was the least. In addition, Padina pavonica was confirmed to be a safe compound through cytotoxicity testing. After conducting a sensory evaluation test, it was confirmed that Padina pavonica in meat products proved to be a satisfactory product.

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Immune stimulatory and anti-HIV-1 potential of extracts derived from marine brown algae Padina tetrastromatica

Abstract: AbstractBackgroundMarine brown algae are biologically diverse and their medicinal value has been explored limited. We assessed whether Padina tetrastromatica Hauck will possess the immune stimulatory and human immunodeficiency virus (HIV-1) inhibitory activity.M… Show more

Cited by 9 publications

References 35 publications

Secondary metabolites produced by endophytic fungi, Alternaria alternata, as potential inhibitors of the human immunodeficiency virus

Secondary metabolites produced by endophytic fungi, Alternaria alternata, as potential inhibitors of the human immunodeficiency virus

Antioxidant, antidiabetic, anti-inflammatory and anticancer potential of some seaweed extracts

Utilization of Algae Extracts as Natural Antibacterial and Antioxidants for Controlling Foodborne Bacteria in Meat Products

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