Immune TB Antibody Phage Display Library as a Tool To Study B Cell Immunity in TB Infections

Abstract

B cells and in particular antibodies has always played second fiddle to cellular immunity in regard to tuberculosis (TB). However, recent studies has helped position humoral immunity especially antibodies back into the foray in relation to TB immunity. Therefore, the ability to correlate the natural antibody responses of infected individuals toward TB antigens would help strengthen this concept. Phage display is an intriguing approach that can be utilized to study antibody-mediated responses against a particular infection via harvesting the B cell repertoire from infected individuals. The development of disease-specific antibody libraries or immune libraries is useful to better understand antibody-mediated immune responses against specific disease antigens. This study describes the generation of an immune single-chain variable fragment (scFv) library derived from TB-infected individuals. The immune library with an estimated diversity of 10 independent clones was then applied for the identification of monoclonal antibodies against Mycobacterium tuberculosis α-crystalline as a model antigen. Biopanning of the library isolated three monoclonal antibodies with unique gene usage. This strengthens the role of antibodies in TB immunity in addition to the role played by cellular immunity. The developed library can be applied against other TB antigens and aid antibody-derived TB immunity studies in the future.

Development of a Phage Display Panning Strategy Utilizing Crude Antigens: Isolation of MERS-CoV Nucleoprotein human antibodies

“…The main assurance is that these proteins are mainly prepared with good purity and yield. This is evident with the reports of antibody development for dengue virus 10 , herpes simplex virus 11 , cytomegalovirus 11 , Zika virus 12 , Ebola virus 7,13 , Plasmodium species 14 , Mycobacterium tuberculosis 15 , and nematodes responsible for filariasis 16 .…”

Developing Recombinant Antibodies by Phage Display Against Infectious Diseases and Toxins for Diagnostics and Therapy

“…The avidity effect leading to cooperative binding resulted in a binding signal increase of ~14 times in ELISA. Antibodies against Mtb were also generated by Hamidon and colleagues ( Hamidon et al., 2018 ) utilizing a different approach. A patient immune TB library with a nominal diversity of 10 9 different scFv antibodies could be generated starting from the B-cell material from six individuals infected with TB.…”