Immunization against Rauscher Mouse Leukemia with Tissue Culture Material

Barski, G; Youn, Jung Koo

doi:10.1126/science.149.3685.751

Cited by 25 publications

(10 citation statements)

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“…In in vivo experiments with two variants of Rauscher leukaemia virus (RV) previously reported from this laboratory (Barski and Youn, 1965), it was observed that preventive inoculation with a low leukaemogenic variant of RV protected mice against subsequent challenge with the virulent form. Later, these observations were confirmed and it became clear that the mechanism was of an immunological nature, probably involving both circulating antibodies and sensitized lymphoid cells (Youn et al, 1968).…”

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In vitro interference between low and high leukaemogenic variants of rauscher virus

Boyd

Youn

Barski

1973

Intl Journal of Cancer

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Cultures of the virus-free P4bis line, originating from a C57BI mouse, were infected in vitro with a '' low leukaemogenic " variant ( R C ) of the Rauscher leukaemia virus ( R V ) , prepared from long-term cultures of this virus in syngeneic cells. Later, after different delays, these infected cultures (P4bis+ R C ) were superinfected with a highly pathogenic variant ( R R ) of the R V , obtained from short-term cultures of R V extracted from spleens of leukaemic mice. Eighteen days afer these superinfections with R R , supernatants of the doubly-infected cell cultures (P4bisS-RC+ R R ) and their corresponding controls were assayed for leukaemogenic potential by intraperitoneal inoculation into young adult BALBIc mice. The proportion of mice which had developed leukaemia by the 120th day after inoculation served for the evaluation of in vitro interference between the R C and R R virus variants. Even when the in vitro infection of cells with RC virus was followed only I h later by the R R superinfection, there was a significant difference between the proportion of ieukaemic animals in the group inoculated with P4bis+ RC+ R R supernatant (56%) and that in the P4bisf R R positive controlgroup

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In vitro interference between low and high leukaemogenic variants of rauscher virus

Boyd

Youn

Barski

1973

Intl Journal of Cancer

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“…However, on different occasions, active immunization methods have been used, such as: (1) syngeneic transplantable leukaemia in sub-threshold doses (Axelrad, 1963;Klein & Klein, 1964;Pasternak & Graffi, 1963); (2) allogeneic transplantable leukaemia (Klein & Klein, 1964;McCoy et al, 1967;Pasternak & Graffi, 1963); (3) culture cells chronically infected with virus (Barski & Youn, 1965;Mayyasi et al, 1968); (4) infectious leukaemia virus (Bianco et al, 1966;Glynn et al, 1964Glynn et al, , 1968Klein & Klein, 1964;Mayyasi & Moloney, 1967;McCoy et al, 1967;Sachs, 1962;Slettenmark & Klein, 1962); (5) attenuated leukaemia virus (Fink & Rauscher, 1964;Friend, 1959;Huebner et al, 1976;Kelloff et al, 1976;Mayyasi & Moloney, 1967;McCoy et al, 1967;Tyndall, et al, 1967); (6) infectious murine sarcoma virus Huebner et al, 1976;Basombrio et al, 1977); and (7) purified viral components (Hunsmann et al, 1975;Ihle et al, 1976a,b). The variability among experimental protocols makes a direct comparison of the different results extremely difficult.…”

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Immunization against primary, transplanted and spontaneous murine leukaemia using a live Moloney sarcoma virus vaccine

Mayer¹,

Basombrío²,

Pasqualini³

1980

Br J Cancer

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As for spontaneous leukaemias, immunized BALB/c mice showed an increased incidence over the controls, while in Fl (Swiss x AKR) mice the incidence was similar but the latent period was shorter. Furthermore, in long-term observations the MSV-M-immunized mice showed an increased mortality, which could be related to (1) new phenotypic mixtures between MSV-M and leukaemia viruses; (2) reactivation of MSV-M sarcoma-genesis with age, and (3) genotype susceptibility to MSV-M.To DATE, no standardized system has been used to compare the results of one and the same immunization protocol on the prevention of transplanted, primary and spontaneous leukaemia in the mouse.

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“…Previous investigations have shown that murine leukemia viruses (MuLV) lose leukemogenic potential after a number of in vitro passages (Barski and Youn, 1965; Sinkovics et al, 1966;Wright and Lasfargues, 1966;Friend et al, 1966;Tkaczevski et al, 1968;Yoshikura et a/., 1969;Kirsten et al, 1974), or even a single passage (Schlom ef al., 1971). S:ill, high-and lowleukemogenic viruses have been indistinguishable from each other by ultrastructural (Tkaczevski et a/., 1968), antigenic, infectivity and replication rate characteristics (Barski and Youn, 1971 ; Barbieri et al, 1971 ;Barski et al, 1973).…”

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A quantitative comparison between in vivo‐and in vitro‐derived friend leukemia virus

Evenson

Pla

Beju

et al. 1975

Intl Journal of Cancer

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The biological activities of RNA viruses derived from Friend leukemia cells in culture (TCV) were compared with those of viruses derived from the plasma (PV) of mice infected with Friend leukemia virus (FLV). The comparison was quantitatively based on the actual number of viruses used in each experiment as determined by counting under the electron microscope. Electron microscopy also provided a qualitative assessment of the structural integrity of the concentrated virus particles used in various bioassays. The data shows that the leukemogenic and spleen-focus-forming (SFF) activities of TCV, although demonstrable, are respectively 10(5) and 10(4) lower than those of PV. Moreover, TCV has 10(4) less helper activity (S+L- test) than PV. The level of reverse transcriptase activity is ten times lower in TCV than in PV which indicates that there is little correlation between polymerase activity and the other biological activities measured. The decreased biological activity of the in vitro grown virus is thought to be intrinsic to this type of virus although all extrinsic factors have not been ruled out.

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Immunization against Rauscher Mouse Leukemia with Tissue Culture Material

Cited by 25 publications

References 1 publication

In vitro interference between low and high leukaemogenic variants of rauscher virus

In vitro interference between low and high leukaemogenic variants of rauscher virus

Immunization against primary, transplanted and spontaneous murine leukaemia using a live Moloney sarcoma virus vaccine

A quantitative comparison between in vivo‐and in vitro‐derived friend leukemia virus

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