Immunization effect of recombinant P27/30 protein expressed in Escherichia coli against the hard tick Haemaphysalis longicornis (Acari: Ixodidae) in rabbits

Abstract

Abstract:We investigated the induction of resistance to Haemaphysalis longicornis infestation in rabbits that had been immunized with recombinant H. longicornis P27/30 protein. The success of immunological control methods is dependent upon the use of potential key antigens as tick vaccine candidates. Previously, we cloned a gene encoding 27 kDa and 30 kDa proteins (P27/30) of H. longicornis, and identified P27/30 as a troponin I-like protein. In this study, rabbits that were immunized with recombinant P27/30 expressed in Escherichia coli showed the statistically significant longer feeding duration for larval and adult ticks (P<0.05), low engorgement rates in larval ticks (64.4%), and an apparent reduction in egg weights, which suggest that H. longicornis P27/30 protein is a potential candidate antigen for a tick vaccine. These results demonstrated that the recombinant P27/30 protein might be a useful vaccine candidate antigen for biological control of H. longicornis.

Recombinant Rhipicephalus appendiculatus gut (Ra86) and salivary gland cement (Trp64) proteins as candidate antigens for inclusion in tick vaccines: protective effects of Ra86 on infestation with adult R. appendiculatus

“…The protective mechanism in the host is likely to be at least partially a result of lysis of tick gut epithelial cells through the anti-Ra86 antibodies present in the blood meal taken from vaccinated rabbits, resulting in reduced tick survival and fertility. Such protective mechanisms have also been reported by de la Fuente et al 9 and Almazan et al 33 Similar results were reported by You 40 and Kumar et al, 41 where antibodies were consumed by ticks together with the blood meal and were postulated to damage gut digestive cells, thereby affecting transport of nutrients and tick reproductive performance.…”

Characterization and evaluation of a Sarcoptes scabiei allergen as a candidate vaccine

“…Furthermore, tropomyosin has been demonstrated to effectively induce protective responses against other parasites such as the filarial nematode A. viteae as mentioned above. The troponin I-like protein of Haemaphysalis longicornis , which has been considered as a potential vaccine candidate antigen, may be identical to tropomyosin [ 33 ]. Hence, we chose the recombinant protein as a potential candidate antigen.…”

A house dust mite allergen homologue from poultry red mite Dermanyssus gallinae (De Geer)*

“…The presence of tick paramyosin in the gut, its potential exposure to ingested host blood and the demonstrated ability to interact with components of the host's immune system has led to the suggestion that it may represent an important new ‘concealed’ antigen for vaccination studies (18). In addition, recombinant versions of the muscle‐associated protein P27/30, a troponin I‐like molecule, have recently been used successfully to produce a protective response in rabbits and mice against infestation with the tick Haemaphysalis longicornis (19,20). Troponin I is a member of a complex containing troponin C which binds Ca 2+ , troponin I which binds actin (thus inhibiting actin–myosin interactions), and troponin T which binds to tropomyosin (21).…”