Immunization of dogs with a DNA vaccine induces protection against rabies virus

Perrin, Pierre; Jacob, Yves; Aguilar-Setién, Álvaro; Loza-Rubio, Elizabeth; Jallet, Corinne; Desmézières, Emmanuel; Aubert, Michel; Cliquet, Florence; Tordo, Noël

doi:10.1016/s0264-410x(99)00247-9

Cited by 72 publications

(34 citation statements)

References 17 publications

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“…Evidence suggests these vaccines are fully effective against virtually all RABVs tested to date (4,11,33,37,46,50) but not against distantly related viruses in phylogroup II or WCBV (5,43). In addition, the reported variable efficacy of vaccines against the EBLVs and DUVV (11,17,32) and recent evidence of variable efficacy against ARAV, KHUV, and IRKV (22) suggest that there may be a gradual loss of protection as viruses become antigenically distant from vaccine strains.…”

Section: Discussionmentioning

confidence: 99%

Quantifying Antigenic Relationships among the Lyssaviruses

Horton

McElhinney

Marston

et al. 2010

J Virol

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All lyssaviruses cause fatal encephalitis in mammals. There is sufficient antigenic variation within the genus to cause variable vaccine efficacy, but this variation is difficult to characterize quantitatively: sequence analysis cannot yet provide detailed antigenic information, and antigenic neutralization data have been refractory to high-resolution robust interpretation. Here, we address these issues by using state-of-the-art antigenic analyses to generate a high-resolution antigenic map of a global panel of 25 lyssaviruses. We compared the calculated antigenic distances with viral glycoprotein ectodomain sequence data. Although 67% of antigenic variation was predictable from the glycoprotein amino acid sequence, there are in some cases substantial differences between genetic and antigenic distances, thus highlighting the risk of inferring antigenic relationships solely from sequence data at this time. These differences included epidemiologically important antigenic differences between vaccine strains and wild-type rabies viruses. Further, we quantitatively assessed the antigenic relationships measured by using rabbit, mouse, and human sera, validating the use of nonhuman experimental animals as a model for determining antigenic variation in humans. The use of passive immune globulin is a crucial component of rabies postexposure prophylaxis, and here we also show that it is possible to predict the reactivity of immune globulin against divergent lyssaviruses.

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Section: Discussionmentioning

confidence: 99%

Quantifying Antigenic Relationships among the Lyssaviruses

Horton

McElhinney

Marston

et al. 2010

J Virol

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“…37 Murine models have been instrumental to show that anti-specific HPV responses can be obtained by DNA immunization, which can both control tumor and metastasis development and promote tumor regression. [38][39][40][41] DNA vaccines have also been tested in rabbits 42,43 and dogs 44 with similar results. Altogether, the encouraging results obtained with different animal models have warranted the development of HPV vaccines in humans.…”

Section: Development Of Papillomavirus Vaccines Animal Modelsmentioning

confidence: 77%

Vaccines against papillomavirus infections and disease

Villa

2003

Salud pública Méx

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“…The DNA vaccine against rabies was constructed following technical procedures previously described by Bahloul et al [6] and Perrin et al [33]. It consists of a mammalian expression vector pClneo (Promega Corporation, Madison, WI., USA) into which the glycoprotein-encoding gene of the HQ01-IMSS virus [3] was inserted (pGQH).…”

Section: Plasmid Constructionmentioning

confidence: 99%

“…Furthermore, some rabies vaccines have been associated with injection-site fibrosarcomas in cats [34]. These problems have led to continued efforts to develop safer rabies vaccines using recombinant subunit proteins [39], recombinant viral vectors [30,41], and deoxyribonucleic acid (DNA) based vaccines [10,13,33,40].…”

Section: Introductionmentioning

confidence: 99%

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Intradermal DNA vaccination in ear pinnae is an efficient route to protect cats against rabies virus

et al. 2008

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-A DNA vaccine against rabies (pGQH) was administrated to cats in order to examine different administration routes. Four groups of three cats each were inoculated with pGQH as follows: group A, intramuscularly (IM), 100 µg; group B, intranasally (IN), 100 µg; group C, intradermally into ear pinnae (ID-EP), 100 µg, and group D, IM, 200 µL of phosphate buffer solution (PBS) alone (control group). Blood was drawn on days 0, 30, 60, 90, 120, 150, and 180. Groups A, B, and C received a booster on day 30. At day 200 all animals were challenged. A passive transfer of cat sera, as well as a viral challenge, was performed in mice. The results displayed that neutralizing antibody titers were higher in cats of group C (ID-EP) showing high early titers (> 2 IU) and the highest titer was on day 120 (> 14 IU). In group B (IN), two out of three cats seroconverted on day 30 (> 0.5 IU), the third cat seroconverted until day 60 (> 0.5 IU). In contrast, the lowest levels of neutralizing antibodies were detected in group A (IM). The control group showed no anti-rabies antibodies. Groups A (IM) and D (control) succumbed after lethal challenge. All animals from the ID-EP group (C) survived, only one individual from the IN (B) group died. Mice that received cat sera from ID-EP, IM, and IN groups survived and were protected (30/30 survivors). Mice groups that received pre-immunization sera from cats were not protected (0/30 survivors). This study demonstrates that pGQH immunization was successful when it was administrated ID-EP, and acceptable through the IN route. The IM route, however, was not effective in cats. For vaccination, the IN route seems attractive due to its accessibility for application, but it seems to activate seroconversion slowly. The best route to promote anti-rabies antibody titers was the ID-EP route. This practical and efficient route should be further studied.rabies / DNA vaccine / intradermal into ear pinna / intranasal / cats

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Immunization of dogs with a DNA vaccine induces protection against rabies virus

Cited by 72 publications

References 17 publications

Quantifying Antigenic Relationships among the Lyssaviruses

Quantifying Antigenic Relationships among the Lyssaviruses

Vaccines against papillomavirus infections and disease

Intradermal DNA vaccination in ear pinnae is an efficient route to protect cats against rabies virus

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