Immunization of Man against Falciparum and Vivax Malaria by Use of Attenuated Sporozoites *

Clyde, David F.; Rm, Miller; Woodward, William E.; McCarthy, Vincent C.

doi:10.4269/ajtmh.1975.24.397

Cited by 358 publications

(168 citation statements)

References 0 publications

Supporting

Mentioning

161

Contrasting

Unclassified

Order By: Relevance

“…The feasibility for the development of a malaria vaccine was first suggested when it was shown that immunization with irradiated Plasmodium spp. sporozoites could protect mice, monkeys, and humans from the development of malaria after challenge with live sporozoites (6,7). Subsequent studies indicated that CD8 ϩ T cells play an important role in this immunity, targeting the liver stages of the disease, and preventing the development of the pathogenic blood stages (8 -13).…”

mentioning

confidence: 99%

The Dendritic Cell-Specific Chemokine, Dendritic Cell-Derived CC Chemokine 1, Enhances Protective Cell-Mediated Immunity to Murine Malaria

Bruña–Romero

Schmieg

Val

et al. 2003

The Journal of Immunology

View full text Add to dashboard Cite

Cell-mediated immunity plays a crucial role in the control of many infectious diseases, necessitating the need for adjuvants that can augment cellular immune responses elicited by vaccines. It is well established that protection against one such disease, malaria, requires strong CD8+ T cell responses targeted against the liver stages of the causative agent, Plasmodium spp. In this report we show that the dendritic cell-specific chemokine, dendritic cell-derived CC chemokine 1 (DC-CK1), which is produced in humans and acts on naive lymphocytes, can enhance Ag-specific CD8+ T cell responses when coadministered with either irradiated Plasmodium yoelii sporozoites or a recombinant adenovirus expressing the P. yoelii circumsporozoite protein in mice. We further show that these enhanced T cell responses result in increased protection to malaria in immunized mice challenged with live P. yoelii sporozoites, revealing an adjuvant activity for DC-CK1. DC-CK1 appears to act preferentially on naive mouse lymphocytes, and its adjuvant effect requires IL-12, but not IFN-γ or CD40. Overall, our results show for the first time an in vivo role for DC-CK1 in the establishment of primary T cell responses and indicate the potential of this chemokine as an adjuvant for vaccines against malaria as well as other diseases in which cellular immune responses are important.

show abstract

mentioning

confidence: 99%

The Dendritic Cell-Specific Chemokine, Dendritic Cell-Derived CC Chemokine 1, Enhances Protective Cell-Mediated Immunity to Murine Malaria

Bruña–Romero

Schmieg

Val

et al. 2003

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…It was shown by using irradiated or genetically attenuated sporozoites that sterile protection can be achieved by the induction of a T-cell response directed against infected hepatocytes [10][11][12][13]. In addition, a liver-stage-specific vaccine has the advantage that it could prevent the pathology associated with the blood-stage of the disease.…”

Section: Introductionmentioning

confidence: 99%

“…It is still under debate whether malaria parasites have evolved specific immune evasion mechanisms that interfere with memory formation. There are several reports of previously immune immigrants losing their immunity to malaria after a certain time of residence in a nonendemic area; however, systematic surveys imply that previously immune adults residing in nonendemic areas maintain a lower risk of severe and fatal disease [7][8][9].It was shown by using irradiated or genetically attenuated sporozoites that sterile protection can be achieved by the induction of a T-cell response directed against infected hepatocytes [10][11][12][13]. In addition, a liver-stage-specific vaccine has the advantage that it could prevent the pathology associated with the blood-stage of the disease.…”

mentioning

confidence: 99%

Plasmodium berghei sporozoite challenge of vaccinated BALB/c mice leads to the induction of humoral immunity and improved function of CD8⁺ memory T cells

et al. 2013

View full text Add to dashboard Cite

Protection against malaria can be achieved by induction of a strong CD8 + T-cell response against the Plasmodium circumsporozoite protein (CSP), but most subunit vaccines suffer from insufficient memory responses. In the present study, we analyzed the impact of postimmunization sporozoite challenge on the development of long-lasting immunity. BALB/c mice were immunized by a heterologous prime/boost regimen against Plasmodium berghei CSP that induces a strong CD8 + T-cell response and sterile protection, which is short-lived. Here, we show that protective immunity is prolonged by a sporozoite challenge after immunization. Repeated challenges induced sporozoite-specific antibodies that showed protective capacity. The numbers of CSP-specific CD8 + T cells were not substantially enhanced by sporozoite infections; however, CSP-specific memory CD8 + T cells of challenged mice displayed a higher cytotoxic activity than memory T cells of immunized-only mice. CD4 + T cells contributed to protection as well; but CD8 + memory T cells were found to be the central mediator of sterile protection. Based on these data, we suggest that prolonged protective immunity observed after immunization and infection is composed of different antiparasitic mechanisms including CD8 + effector-memory T cells with increased cytotoxic activity as well as CD4 + memory T cells and neutralizing antibodies. Keywords: CD8 + T cells r Plasmodium r Sporozoites r VaccinationAdditional supporting information may be found in the online version of this article at the publisher's web-site Correspondence: Dr. Susanne Tartz e-mail: tartz@bni-hamburg.de IntroductionThere is now good progress both in the prevention and the treatment of malaria that leads to decreasing numbers of malaria cases and fatalities; nevertheless malaria is still one of the biggest C 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.eji-journal.eu 694Susanne Tartz et al. Eur. J. Immunol. 2013. 43: 693-704 challenges for global public health management. There are around 250 million cases per year worldwide with the vast majority of these in the African region and it is estimated that malaria accounts for 900 000 deaths annually [1]. Therefore a vaccine is still the ultimate goal of malaria research. The development of immunity and immunological memory to Plasmodium infections in malaria endemic areas has been intensively studied [2]. Children in the age of 6 months to 5 years are at the highest risk of being infected with Plasmodium and developing severe disease. In the first years of life, an anti-disease immunity is acquired that leads to high-density infections with mild symptoms. In contrast, the acquisition of anti-parasite immunity takes years to decades, although transmigrant studies show that adults may acquire immunity more rapidly than children [3,4]. It is supposed that anti-parasite immunity is based mainly on antibodies recognising variant surface antigens on the membranes of infected erythrocytes whereas immune responses against preerythrocytic forms seem to play a m...

show abstract

“…Article available at http://www.parasite-journal.org or http://dx.doi.org/10.1051/parasite/1989646412 et des sporozoïtes (Clyde et al, 1975et Nussenzweig, 1977 permettront dans l'avenir de proposer une nouvelle stratégie de lutte contre le paludisme basée sur l'immuno thérapie. De plus les résultats obtenus avec les immunisations à base de gamètes chez P. gallinaceum (Carter et al, 1976et Gwadz, 1976 et chez P. yoelii (Mendis et al, 1979) ont montré que l'immunité induite était capable de réduire la pro duction du nombre d'oocystes dans l'estomac du moustique.…”

Section: Introductionunclassified

Inhibition complète de la transmission cyclique par immunisation contre les ookinètes dePlasmodium bergheiAnka

Said

Wéry

1989

Ann. Parasitol. Hum. Comp.

View full text Add to dashboard Cite

A. SAID, M. WERY RÉSUMÉ. Un antisérum dirigé contre les ookinètes de P. berghei Anka reconnaît spécifiquement les ookinètes et les zygotes. Cette réaction sérologique a été révélée par la technique d'immuno fluorescence indirecte.L'action des anticorps anti-ookinètes sur la sporogonie a été étudiée sur des moustiques nourris sur des souris immunisées. Un immun-sérum anti-ookinètes titré à 1/320 en immuno fluorescence indirecte obtenu après 6 immunisations a été capable de bloquer complètement la transmission cyclique.Ce type d'immun-sérum pourrait être utilisé pour passer au crible les librairies génomiques ou de cDNA de P. berghei afin d'identifier et de cloner les séquences qui interviennent dans le processus de l'infectivité des gamétocytes. Mots-clés : Plasmodium berghei. Ookinètes. Sérum anti-ookinète. Immunisation. Arrêt de la transmission. Cyclical transmission blocked by immunization against P. berghei Anka ookinetesSUMMARY. An antiserum raised against P. berghei Anka ookinetes binds specifically to ookinetes and zygotes surface as assessed by indirect immunofluorescence. The transmission blocking activity of this serum was assessed by feeding mosquitoes on immunized mice. A serum showing an antibody titer of 1/320 reached after 6 immunizations completely blocks ookinetes development. This immune serum might be used to screen P. berghei genomic or cDNA library in order to identifyi or clone sequences involved in the process of game tocyte infectivity.

show abstract

Immunization of Man against Falciparum and Vivax Malaria by Use of Attenuated Sporozoites *

Cited by 358 publications

References 0 publications

The Dendritic Cell-Specific Chemokine, Dendritic Cell-Derived CC Chemokine 1, Enhances Protective Cell-Mediated Immunity to Murine Malaria

The Dendritic Cell-Specific Chemokine, Dendritic Cell-Derived CC Chemokine 1, Enhances Protective Cell-Mediated Immunity to Murine Malaria

Plasmodium berghei sporozoite challenge of vaccinated BALB/c mice leads to the induction of humoral immunity and improved function of CD8⁺ memory T cells

Inhibition complète de la transmission cyclique par immunisation contre les ookinètes dePlasmodium bergheiAnka

Contact Info

Product

Resources

About

Immunization of Man against Falciparum and Vivax Malaria by Use of Attenuated Sporozoites *

Cited by 358 publications

References 0 publications

The Dendritic Cell-Specific Chemokine, Dendritic Cell-Derived CC Chemokine 1, Enhances Protective Cell-Mediated Immunity to Murine Malaria

The Dendritic Cell-Specific Chemokine, Dendritic Cell-Derived CC Chemokine 1, Enhances Protective Cell-Mediated Immunity to Murine Malaria

Plasmodium berghei sporozoite challenge of vaccinated BALB/c mice leads to the induction of humoral immunity and improved function of CD8+ memory T cells

Inhibition complète de la transmission cyclique par immunisation contre les ookinètes dePlasmodium bergheiAnka

Contact Info

Product

Resources

About

Plasmodium berghei sporozoite challenge of vaccinated BALB/c mice leads to the induction of humoral immunity and improved function of CD8⁺ memory T cells