IMMUNIZATION OF MICE WITH THE FIBRONECTIN‐BINDING PROTEIN AND CLUMPING FACTOR FROM STAPHYLOCOCCUS AUREUS: ANTIBODY RESPONSE AND RESISTANCE AGAINST INTRAPERITONEAL INFECTION

Abstract

The antibody response against Staphylococcus aureus antigens was quantitated in mice immunized with live staphylococci, the fibronectin‐binding protein, clumping factor, or saline by means of enzyme‐linked immunosorbent assay. Immunization with purified clumping factor induced a significant increase in antibody levels, while the fibronectin‐binding protein gave a poor response. All mice immunized with live S. aureus developed a high antibody level. The immunization of mice with live S. aureus or clumping factor resulted in an increased resistance towards staphylococcal intraperitoneal infection.

COMPLEMENT ACTIVATION BY CLUMPING FACTOR AND PROTEIN A FROM STAPHYLOCOCCUS AUREUS STRAIN E 2371

“…4), indicating that CF has a higher capacity on molar level as M, of CF is 400,000 and M, of protein A is 52,000. The CF preparation used in the present study has previously been demonstrated to bind purified fibrinogen, to inhibit the fibrinogen-induced s. aureus clumping, to be highly immunogenic in mice and rabbits, and to induce resistance against S. aureus intraperitoneal infection in mice immunized 3 times intracutaneously with CF (7,8). These results in combination with the present findings indicate that this biological active component may play a role in staphylococcal infections.…”

“…The plates were washed three times as described (8), and incubated 1 h at 20 "C with 0.1 ml of peroxidase conjugated rabbit antibodies against goat immunoglobulins (DAKO, Copenhagen, Denmark). The following washing procedure, enzyme reaction, and determination of the absorbance at 493 nm have been described previously (8).…”

Which are important targets in development of S. aureus mastitis vaccine?

“…In addition, immunization with these MAbs combined with CP5 antibodies sterilized a significant In murine models, the first in vivo research on direct immunization against FnBPs showed 211 underwhelming results (Espersen and Clemmensen, 1985) and passive immunization seemed to be 212 more promising (Rozalska and Wadstrom, 1993). Nevertheless, further studies reported that 213 vaccinated mice develop less severe infections than unvaccinated controls after experimental 214 endocarditis (Schennings et al, 1993) and experimental mastitis (Mamo et al, 1994).…”

“…However, when S. aureus isolates were 135 challenged with a hyper immune serum obtained by SpA recombinant adhesin, the response 136 observed was strongly influenced by isolates SpA gene polymorphisms (Scarpa et al, 2010). Recent Several studies regarding vaccination with ClfA in murine models are available in the literature and 165 the first in vivo research was conducted at least 30 years ago (Espersen and Clemmensen, 1985). 166 However, few data are available for ClfA and ClfB immunization in bovine mastitis models.…”