Immunization With a Combination of 2 Peptides Derived From the C5a Receptor Significantly Reduces Early Atherosclerotic Lesion in Ldlr ^tm1Her Apob ^tm2Sgy J Mice

Abstract

Objective-The goal of this study was to assess whether immunization of Ldlr tm1Her Apob tm2Sgy J mice with 2 peptides located at the N-terminus of the C5a receptor (C5aR), either alone or in combination, is effective in reducing atherosclerotic lesions. Methods and Results-Five-to 6-week-old female Ldlr

Expression of Human Tissue Factor Pathway Inhibitor on Vascular Smooth Muscle Cells Inhibits Secretion of Macrophage Migration Inhibitory Factor and Attenuates Atherosclerosis in ApoE −/− Mice

“…10,12,17 Briefly, tissues were snap-frozen in dry ice, embedded in optimum cutting temperature compound (VWR International, Dorset, UK), sectioned at 5-μm thickness, and fixed in methanol at -20°C. Frozen sections were immersed in 1% BSA-PBS for 30 minutes and then incubated overnight at 4°C with the antibodies defined in the online-only Data Supplement.…”

The complement system and toll-like receptors as integrated players in the pathophysiology of atherosclerosis

“…As C5-inhibitors have been shown to reduce atherosclerosis in murine models indicating C5 as a possible therapeutic target [175,176], several groups have studied inhibition of cleavage of C5 into C5a and C5b, in humans. Two early trials tested pexelizumab, a precursor of the recombinant anti-C5 antibody eculizumab, in coronary artery bypass grafting (CABG) with results indicating reduced mortality [177,178].…”

Vaccination to modulate atherosclerosis

“…This finding suggests that vaccine-induced anti-pneumococcus antibody cross-reactive with oxLDL may have an atheroprotective potential. In two unconfirmed studies, immunization with complement C5a receptor peptide with alum adjuvant was atheroprotective compared to KLH with alum (109). Immunization with rat MDA-modified fibronectin reduced atherosclerosis in descending aorta and subvalvular lesion (110).…”