Immunization with a plasmid DNA containing the gene of trans-sialidase reduces Trypanosoma cruzi infection in mice

Costa, Fábio T. M.; Franchin, Giovanni; Pereira-Chioccola, Vera Lúcia; Ribeirão, Marcelo; Schenkman, Sérgio; Rodrigues, Maurício M.

doi:10.1016/s0264-410x(97)00277-6

Cited by 111 publications

(132 citation statements)

References 15 publications

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“…In studies conducted in our laboratory we used a gene encoding for the catalytic domain of the enzyme trans-sialidase (TS). Immunization of BALB/c mice with the TS gene elicited specific antibodies and promoted T-cell activation (Costa et al 1998). The T-cell immune response was later characterized as being mediated mainly by CD4 Th1 and CD8 TC1 cells, which secreted a large amount of IFN-γ , but not IL-4 or IL-10 ( Rodrigues et al 1999).…”

Section: Trypanosoma Cruzimentioning

confidence: 99%

“…The T-cell immune response was later characterized as being mediated mainly by CD4 Th1 and CD8 TC1 cells, which secreted a large amount of IFN-γ , but not IL-4 or IL-10 ( Rodrigues et al 1999). Upon challenge with infective T. cruzi trypomastigotes, immunized mice showed a significant reduction in parasitemia and survived acute lethal infection (Costa et al 1998).…”

Section: Trypanosoma Cruzimentioning

confidence: 99%

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Importance of CD8 T cell-mediated immune response during intracellular parasitic infections and its implications for the development of effective vaccines

Rodrigues

Boscardin

Vasconcelos

et al. 2003

An. Acad. Bras. Ciênc.

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Obligatory intracellular parasites such as Plasmodium sp, Trypanosoma cruzi, Toxoplasma gondii and Leishmania sp are responsible for the infection of hundreds of millions of individuals every year. These parasites can deliver antigens to the host cell cytoplasm that are presented through MHC class I molecules to protective CD8 T cells. The in vivo priming conditions of specific CD8 T cells during natural infection are largely unknown and remain as an area that has been poorly explored. The antiparasitic mechanisms mediated by CD8 T cells include both interferon-γ -dependent and -independent pathways. The fact that CD8 T cells are potent inhibitors of parasitic development prompted many investigators to explore whether induction of these T cells can be a feasible strategy for the development of effective subunit vaccines against these parasitic diseases. Studies performed on experimental models supported the hypothesis that CD8 T cells induced by recombinant viral vectors or DNA vaccines could serve as the basis for human vaccination. Regimens of immunization consisting of two different vectors (heterologous prime-boost) are much more efficient in terms of expansion of protective CD8 T lymphocytes than immunization with a single vector. The results obtained using experimental models have led to clinical vaccination trials that are currently underway.

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Section: Trypanosoma Cruzimentioning

confidence: 99%

Section: Trypanosoma Cruzimentioning

confidence: 99%

Importance of CD8 T cell-mediated immune response during intracellular parasitic infections and its implications for the development of effective vaccines

Rodrigues

Boscardin

Vasconcelos

et al. 2003

An. Acad. Bras. Ciênc.

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“…cruzi, the etiologic agent of Chagas' disease, is still a problem in South American countries, afflicting 16-18 million people and causing thousands of deaths every year. Plasmids containing parasitic genes have been successfully used to elicit protective immunity against experimental T. cruzi infection (8). In this study, we employed a plasmid (p154/13) containing a gene encoding the catalytic domain of T. cruzi transsialidase (TS).…”

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confidence: 99%

Adjuvant Effect of the Propionibacterium acnes and Its Purified Soluble Polysaccharide on the Immunization with Plasmidial DNA Containing a Trypanosoma cruzi Gene

Mussalem

Vasconcelos

Squaiella

et al. 2006

Microbiology and Immunology

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In the present work we investigated the role of killed Propionibacterium acnes or a soluble polysaccharide extracted from bacterium cell wall in modulated experimental immunization with plasmidial DNA. We used a plasmid, p154/13, containing a gene‐encoding catalytic domain of Trypanosoma cruzi (T. cruzi) trans‐sialidase. As previously described, immunization of BALB/c mice with p154/13 elicited humoral, cell‐mediated and protective immune responses against T. cruzi infection. In this study we describe that both P. acnes and its soluble polysaccharide fraction have the ability to modulate the immune response elicited by p154/13. Treatment with these adjuvants enhanced specific trans‐sialidase Th1 immune response, as revealed by a lower IgG1/IgG2a ratio and stronger in vitro IFN‐γ synthesis by CD4+ T cells. The most important fact was that treatment with P. acnes or its soluble polysaccharide fraction in the presence of p154/13 significantly reduced the peak of parasitemia observed 7 to 8 days after T. cruzi challenge. These data suggest that P. acnes or its soluble polysaccharide fraction may improve the protective potential of a DNA vaccine against experimental T. cruzi infection.

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“…Although vector control programs and the application of chemotherapeutics are partial solutions to control of the infection and disease, these approaches alone are unlikely to be long-term solutions to combating Chagas' disease. Vaccination also has potential for reducing the severity of T. cruzi infection Chagas' disease (2)(3)(4). The design of appropriate vaccination strategies against T. cruzi requires the elucidation of the mechanisms for immune protection.…”

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confidence: 99%

Antigen-Specific Th1 But Not Th2 Cells Provide Protection from Lethal Trypanosoma cruzi Infection in Mice

Kumar

Tarleton

2001

The Journal of Immunology

106

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Infection with Trypanosoma cruzi results in the development of both type 1 and type 2 patterns of cytokine responses during acute and chronic stages of infection. To investigate the role of Th1 and Th2 subsets of CD4+ T cells in determining the outcome of T. cruzi infection in mice, we have developed T. cruzi clones that express OVA and have used OVA-specific TCR-transgenic T cells to generate OVA-specific Th1 and Th2 cells. BALB/c mice receiving 107 OVA-specific Th1 cells and then challenged with OVA-expressing T. cruzi G-OVA.GPI showed significantly lower parasitemia and increased survival in comparison to mice that received no cells. In contrast, recipients of OVA-specific Th2 cells developed higher parasitemias, exhibited higher tissue parasitism and inflammation, and had higher mortality than recipients of Th1 cells after infection with T. cruzi G-OVA.GPI. Mice receiving a mixture of both Th1 and Th2 OVA-specific cells also were not protected from lethal challenge. The protective effect of the OVA-specific Th1 cells was OVA dependent as shown by the fact that transfer of OVA-specific Th1 or Th2 cells failed to alter the course of infection or disease in mice challenged with wild-type T. cruzi. Immunohistochemical analysis of OVA-specific Th1 and Th2 cells at 4, 15, and 30 days postinfection revealed the persistence and expansion of these cells in mice challenged with T. cruzi G-OVA.GPI but not in mice infected with wild-type T. cruzi. We conclude that transfer of Ag-specific Th1 cells but not Th2 cells protect mice from a lethal infection with T. cruzi.

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Immunization with a plasmid DNA containing the gene of trans-sialidase reduces Trypanosoma cruzi infection in mice

Cited by 111 publications

References 15 publications

Importance of CD8 T cell-mediated immune response during intracellular parasitic infections and its implications for the development of effective vaccines

Importance of CD8 T cell-mediated immune response during intracellular parasitic infections and its implications for the development of effective vaccines

Adjuvant Effect of the Propionibacterium acnes and Its Purified Soluble Polysaccharide on the Immunization with Plasmidial DNA Containing a Trypanosoma cruzi Gene

Antigen-Specific Th1 But Not Th2 Cells Provide Protection from Lethal Trypanosoma cruzi Infection in Mice

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