Immunization with Chlamydia psittaci plasmid-encoded protein CPSIT_p7 induces partial protective immunity against chlamydia lung infection in mice

Tan, Yuan; Li, Yumeng; Yang, Zaixing; Yu, Jiangnan; Wen, Yang; Wang, Chuan; Xu, Man; Chen, Qian; Lu, Chunxue; Wu, Yimou

doi:10.1007/s12026-018-9018-3

Cited by 8 publications

(11 citation statements)

References 40 publications

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“…For optimal protection, chlamydial vaccines must induce Th1-cytokine-biased CD4 + T cells, especially IFN-γ, and humoral responses [ 17 , 29 , 30 ]. Our previous studies also found that both subunit antigens and polypeptide antigens could induce the secretion of Th1-biased cytokines and produce protective effects [ 19 , 31 ]. In addition, we also detected significant differences in the reduced levels of IFN-γ in lung tissues after C. psittaci infection.…”

Section: Discussionmentioning

confidence: 99%

“…Our previous studies have proven that the C. psittaci plasmid proteins CPSIT_p7 and CPSIT_p8 possess desirable immunogenicity as well. The CPSIT_p7 polypeptide vaccine antigen was further constructed, and animal experiments showed good anti-infection protection [ 18 , 19 ]. Donati et al [ 20 ] constructed a pCMV-KA-Pgp3 eukaryotic vector, and then immunized C3H/HeN mice to evaluate the protective effect against C. trachomatis vaginal infection.…”

Section: Introductionmentioning

confidence: 99%

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Protective Immunity against Chlamydia psittaci Lung Infection Induced by a DNA Plasmid Vaccine Carrying CPSIT_p7 Gene Inhibits Dissemination in BALB/c Mice

Wang

Jin

Jie-wen

et al. 2023

IJMS

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Chlamydia psittaci (C. psittaci), a zoonotic pathogen, poses a potential threat to public health security and the development of animal husbandry. Vaccine-based preventative measures for infectious diseases have a promising landscape. DNA vaccines, with many advantages, have become one of the dominant candidate strategies in preventing and controlling the chlamydial infection. Our previous study showed that CPSIT_p7 protein is an effective candidate for a vaccine against C. psittaci. Thus, this study evaluated the protective immunity of pcDNA3.1(+)/CPSIT_p7 against C. psittaci infection in BALB/c mice. We found that pcDNA3.1(+)/CPSIT_p7 can induce strong humoral and cellular immune responses. The IFN-γ and IL-6 levels in the infected lungs of mice immunized with pcDNA3.1(+)/CPSIT_p7 reduced substantially. In addition, the pcDNA3.1(+)/CPSIT_p7 vaccine diminished pulmonary pathological lesions and reduced the C. psittaci load in the lungs of infected mice. It is worth noting that pcDNA3.1(+)/CPSIT_p7 suppressed C. psittaci dissemination in BALB/c mice. In a word, these results demonstrate that the pcDNA3.1(+)/CPSIT_p7 DNA vaccine has good immunogenicity and immunity protection effectiveness against C. psittaci infection in BALB/c mice, especially pulmonary infection, and provides essential practical experience and insights for the development of a DNA vaccine against chlamydial infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protective Immunity against Chlamydia psittaci Lung Infection Induced by a DNA Plasmid Vaccine Carrying CPSIT_p7 Gene Inhibits Dissemination in BALB/c Mice

Wang

Jin

Jie-wen

et al. 2023

IJMS

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“…Recombinant protein CPSIT_P7 was purified as previously described (Tan et al, 2018), and the purity of the CPSIT_P7 protein was detected by SDS-PAGE. Then, the purified CPSIT_P7 protein was treated with the ToxinEraser TM Endotoxin Removal Kit (GenScript, Piscataway, United States), and the endotoxin level was measured using Limulus amebocyte lysate (Chinese Horseshoe Crab Reagent Manufactory, Ltd., Xiamen, China).…”

Section: Purification Of the Recombinant Cpsit_p7mentioning

confidence: 99%

Chlamydia psittaci Plasmid-Encoded CPSIT_P7 Elicits Inflammatory Response in Human Monocytes via TLR4/Mal/MyD88/NF-κB Signaling Pathway

Chen

Yan

et al. 2020

Front. Microbiol.

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The chlamydial plasmid, an essential virulence factor, encodes plasmid proteins that play important roles in chlamydial infection and the corresponding immune response. However, the virulence factors and the molecular mechanisms of Chlamydia psittaci are not well understood. In the present study, we investigated the roles and mechanisms of the plasmid-encoded protein CPSIT_P7 of C. psittaci in regulating the inflammatory response in THP-1 cells (human monocytic leukemia cell line). Based on cytokine arrays, CPSIT_P7 induces the expression of interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemoattractant protein-1 (MCP-1) in THP-1 cells. Moreover, the expression levels of IL-6, IL-8, and MCP-1 stimulated by CPSIT_P7 declined after silencing of the Toll-like receptor 4 (TLR4) gene using small interfering RNA and transfection of a dominant negative plasmid encoding TLR4 (pZERO-hTLR4). We further demonstrated that transfection with the dominant negative plasmid encoding MyD88 (pDeNy-hMyD88) and the dominant negative plasmid encoding Mal (pDeNy-hMal) could also abrogate the expression of the corresponding proteins. Western blot and immunofluorescence assay results showed that CPSIT_P7 could activate nuclear factor κB (NF-κB) signaling pathways in THP-1 cells. Altogether, our results indicate that the CPSIT_P7 induces the TLR4/Mal/MyD88/NF-κB signaling axis and therefore contributes to the inflammatory cytokine response.

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“…BALB/c mice intranasally immunized with recombinant Pgp3 protein purified from chlamydial serovar D exhibited cross-serovar protection against C. muridarum genital tract infection ( 17 ). Furthermore, immunization with Chlamydia psitaci CPSIT_P7, homologous of C. trachomatis Pgp3 with 70% protein sequence identity, provided partial protection against C. psitaci lung infection in BALB/c mice ( 18 ). Therefore, Pgp3 showed stable protective efficacy against both Chlamydia strains and in different mouse models, suggesting the potential value of Pgp3 in clinical vaccine development.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Pgp3 vaccination for Chlamydia urogenital tract infection depends on its native conformation

et al. 2022

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Urogenital tract infections with Chlamydia trachomatis have frequently been detected among patients diagnosed with sexually transmitted infections, and such infections lead to inflammatory complications. Currently, no licensed chlamydial vaccine is available in clinical practice. We previously reported that immunization with recombinant C. trachomatis plasmid-encoded virulence factor Pgp3 provided cross-serovar protection against C. muridarum genital tract infection. Because Pgp3 is a homotrimer and human antisera only recognize the trimeric form of Pgp3, we compared the effects of the native conformation of Pgp3 (trimer) and heat-denatured Pgp3 (monomer) to determine whether the native conformation is dispensable for the induction of protective immunity against chlamydial vaginal challenge. Both Pgp3 trimer and monomer immunization induced corresponding specific antibody production, but only trimer-induced antibody recognized endogenous Pgp3, and trimer-immunized mouse splenocytes showed the highest IFN-γ production upon restimulation with the chlamydial elementary body or native Pgp3 in vitro. Importantly, only Pgp3 trimer-immunized mice showed shortened lower genital tract chlamydial shedding and decreased upper genital tract pathology. Thus, Pgp3-induced protective immunity against Chlamydia urogenital tract infection is highly dependent on the native conformation, which will guide the design of Pgp3-based polypeptides and multi-subunit chlamydial vaccines.

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Immunization with Chlamydia psittaci plasmid-encoded protein CPSIT_p7 induces partial protective immunity against chlamydia lung infection in mice

Cited by 8 publications

References 40 publications

Protective Immunity against Chlamydia psittaci Lung Infection Induced by a DNA Plasmid Vaccine Carrying CPSIT_p7 Gene Inhibits Dissemination in BALB/c Mice

Protective Immunity against Chlamydia psittaci Lung Infection Induced by a DNA Plasmid Vaccine Carrying CPSIT_p7 Gene Inhibits Dissemination in BALB/c Mice

Chlamydia psittaci Plasmid-Encoded CPSIT_P7 Elicits Inflammatory Response in Human Monocytes via TLR4/Mal/MyD88/NF-κB Signaling Pathway

Efficacy of Pgp3 vaccination for Chlamydia urogenital tract infection depends on its native conformation

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