Immunization with plasmid DNA encoding a truncated, secreted form of the bovine viral diarrhea virus E2 protein elicits strong humoral and cellular immune responses

Liang, Rong; Hurk, J V van den; Zheng, Chunfu; Yu, Hong; Pontarollo, Reno; Babiuk, Lorne A.; Hurk, Sylvia van Drunen Littel‐van den

doi:10.1016/j.vaccine.2005.06.025

Cited by 42 publications

(26 citation statements)

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“…DNA vaccines and recombinant protein vaccines utilize different mechanisms to elicit antigen-specific responses. Due to the production of antigen in transfected cells of the host, a DNA vaccine induces robust T-cell responses, which are critical for the development of T-cell-dependent antibody responses (Liang et al, 2005;Martin et al, 2006). DNA immunization is also highly effective in priming antigen-specific memory B cells.…”

Section: Discussionmentioning

confidence: 99%

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DNA prime–protein boost strategies protect cattle from bovine viral diarrhea virus type 2 challenge

Liang

Hurk

Landi

et al. 2008

Journal of General Virology

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At present, infections with bovine viral diarrhea virus (BVDV) type 2 occur nearly as frequently as those with BVDV type 1, so development of vaccines that protect cattle from both type 1 and type 2 BVDV has become critical. In this study, we compared various DNA prime-protein boost vaccination strategies to protect cattle from challenge with BVDV-2 using the major protective antigen of BVDV, glycoprotein E2. Calves were immunized with a plasmid encoding either type 1 E2 (E2.1) or type 2 E2 (E2.2) or with both plasmids (E2.1+E2.2). This was followed by a heterologous boost with E2.1, E2.2 or E2.1 and E2.2 protein formulated with Emulsigen and a CpG oligodeoxynucleotide. Subsequently, the calves were challenged with BVDV-2 strain 1373. All vaccinated calves developed both humoral and cell-mediated immune responses, including virus-neutralizing antibodies and IFN-c-secreting cells in the peripheral blood. Depletion studies showed that CD4+ T cells were responsible for IFN-c production. Furthermore, the calves vaccinated with either the E2.2 or the E2.1+E2.2 vaccines were very well protected from challenge with BVDV-2, having little leukopenia and showing no weight loss or temperature response. In addition, the animals vaccinated with the E2.1 vaccine were partially protected, so there was a certain level of cross-protection. These data demonstrate that a vaccination strategy consisting of priming with E2.2 or E2.1+E2.2 DNA and boosting with E2.2 or E2.1+E2.2 protein fully protects cattle from BVDV-2 challenge.

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Section: Discussionmentioning

confidence: 99%

“…Peripheral blood was collected from one animal from each of the E2.1+E2.2, E2.2 and placebo groups immediately prior to challenge as well as 14 and 60 days postchallenge. PBMCs were isolated as described previously (Liang et al, 2005), counted and resuspended at a concentration 1610 8 cells ml…”

Section: Methodsmentioning

confidence: 99%

DNA prime–protein boost strategies protect cattle from bovine viral diarrhea virus type 2 challenge

Liang

Hurk

Landi

et al. 2008

Journal of General Virology

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“…Other authors had previously used the signal peptide from a mouse IgG κ light chain [45], from gD of BoHVI [3,42], tPA [42] or from BVDV Erns [52]. In the early E2 construct of Harpin et al [26], the 3' end of the E1 coding sequence was retained at the 5' end of the E2 coding sequence.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, several research teams have reported DNA immunisation to BVDV structural proteins in the mouse [26,42,45,46] and cattle [27,47,42]. All experiments involved E2 constructs from a single BVDV-1a viral strain.…”

Section: Discussionmentioning

confidence: 99%

“…Neutralising antibodies and a cellular response are produced in animals vaccinated with various recombinant viruses expressing E2 [3,17,21], with DNA plasmid constructs expressing E2 [26,42,45] or with E2 subunit vaccines [7,11,12,39,58]. Purified recombinant E2 from BVDV1a strain Singer lead to partial protection against acute infection by the homologous strain [7].…”

Section: Bovine Viral Diarrhoea Virus (Bvdv)mentioning

confidence: 99%

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Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle

et al. 2007

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-We report DNA immunisation experiments in cattle using plasmid constructs that encoded glycoprotein E2 from bovine viral diarrhoea virus (BVDV)-1 (E2.1) and BVDV-2 (E2.2). The coding sequences were optimised for efficient expression in mammalian cells. A modified leader peptide sequence from protein gD of BoHV1 was inserted upstream of the E2 coding sequences for efficient membrane export of the proteins. Recombinant E2 were efficiently expressed in COS7 cells and they presented the native viral epitopes as judged by differential recognition by antisera from cattle infected with BVDV-1 or BVDV-2. Inoculation of pooled plasmid DNA in young cattle elicited antibodies capable of neutralising viral strains representing the major circulating BVDV genotypes.BVDV / DNA immunisation / E2 / neutralisation / synthetic gene

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2009

EFSA Journal

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BACKGROUND AS PROVIDED BY THE COMMISSION Classical swine fever (CSF) is one of the diseases that has caused major socioeconomic damages in the EU during the last decades. Although during the last years considerable progress has been made in the eradication and prevention of the disease, the threat for an epidemic still exists. The main reasons are that CSF virus is still present in feral pigs of some Member States (MSs) and that the virus is endemic in the Balkan region, including the MSs Bulgaria and Romania. Control measures are in place for those areas within the EU but this situation remains a constant threat for new outbreaks in the domestic pig population.

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Immunization with plasmid DNA encoding a truncated, secreted form of the bovine viral diarrhea virus E2 protein elicits strong humoral and cellular immune responses

Cited by 42 publications

References 50 publications

DNA prime–protein boost strategies protect cattle from bovine viral diarrhea virus type 2 challenge

DNA prime–protein boost strategies protect cattle from bovine viral diarrhea virus type 2 challenge

Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle

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