Immunization with Recombinant Sao Protein Confers Protection against
            <i>Streptococcus suis</i>
            Infection

Li, Yuanyi; Gottschalk, Marcelo; Esgleas, Miriam; Lacouture, Sonia; Dubreuil, J. Daniel; Willson, Philip; Harel, Josée

doi:10.1128/cvi.00046-07

Cited by 99 publications

(98 citation statements)

References 47 publications

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“…In contrast, IgG1 (called the "type 2 IgG subclass") elicited during interleukin-4 (IL-4)-dominant Th2 immune responses, usually has a less protective potential. Although the concept of type 1 or type 2 IgG subclasses is not well documented for pigs, recent studies have showed that porcine IgG2 had greater complement-activating capacity than IgG1 (15,16).…”

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confidence: 99%

Antibody Response Specific to the Capsular Polysaccharide Is Impaired in Streptococcus suis Serotype 2-Infected Animals

et al. 2015

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bStreptococcus suis serotype 2 is an extracellular encapsulated bacterium that causes severe septicemia and meningitis in swine and humans. Albeit crucial in the fight against encapsulated bacteria, the nature of the capsular polysaccharide (CPS)-specific antibody (Ab) response during S. suis type 2 infection is unknown. We compared for the first time the features of CPS-specific versus protein-specific Ab responses during experimental infections with live virulent S. suis type 2 in mice. The primary protein-specific Ab response was dominated by both type 1 and 2 IgG subclasses, whereas IgM titers were more modest. The secondary protein-specific Ab response showed all of the features of a memory response with faster kinetics and boosted the titers of all Ig isotypes. In contrast, the primary CPS-specific Ab response was either inexistent or had titers only slightly higher than those in noninfected animals and was essentially composed of IgM. A poor CPS-specific memory response was observed, with only a moderate boost in IgM titers and no IgG. Both protein-and CPS-specific Ab responses were Toll-like receptor 2 independent. By using S. suis type 2 strains of European or North American origin, the poor CPS-specific Ab response was demonstrated to be independent of the genotypic/phenotypic diversity of the strain within serotype 2. Finally, the CPS-specific Ab response was also impaired and lacked isotype switching in S. suis-infected pigs, the natural host of the bacterium. The better resistance of preinfected animals to reinfection with the same strain of S. suis type 2 might thus more likely be related to the development of a protein rather than CPS Ab response.

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Antibody Response Specific to the Capsular Polysaccharide Is Impaired in Streptococcus suis Serotype 2-Infected Animals

et al. 2015

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“…In 2007, Li et al established Sao-based ELISA for detecting titers of Sao-specific total IgG and IgG subclasses in mouse and swine sera [24] . In 2012, Hsueh et al [25] established ELISA using Sao-L and Sao-M for detecting titers of Sao-L-specific total IgG and Sao-M-specific total IgG.…”

Section: Discussionmentioning

confidence: 99%

“…The Sao protein is highly conserved among S. suis strains and Sao-specific antibodies have been shown to react with cell lysates of 28 of 33 S. suis serotypes and 25 of 26 serotype 2 isolates in immunoblots [22] . Sao protein is encoded by three allelic variants of gene of difference lengths, Sao-S (1.5 kb), Sao-M (1.7 kb) and Sao-L (2.0 kb) and Sao-M is the most common type of Sao [24] . Moreover, Sao-M in S. suis is well conserved and the protein exhibits strong immunogenicity [24] .…”

Section: Discussionmentioning

confidence: 99%

“…Sao protein is encoded by three allelic variants of gene of difference lengths, Sao-S (1.5 kb), Sao-M (1.7 kb) and Sao-L (2.0 kb) and Sao-M is the most common type of Sao [24] . Moreover, Sao-M in S. suis is well conserved and the protein exhibits strong immunogenicity [24] . Based on these observations, Sao-M was chosen as a diagnostic antigen for the development of a serodiagnostic test to detect S. suis in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Sao is highly conserved protein among most S. suis strains and has become an important candidate for the subunit S. suis vaccines [22] . Immunization with recombinant Sao protein was capable of provoking strong humoral antibody responses, diminish clinical signs and bacterial dissemination, improve survival rates and provide cross-serotype protection in pig and mouse vaccination protocols [24] , indicating rSao is a suitable antigen for subunit S. suis vaccine development [25] . Sao protein is encoded by three allelic variants of gene of difference lengths, Sao-S (1.5 kb), Sao-M (1.7 kb) and Sao-L (2.0 kb), and Sao-M is the most prevalent variant comprising about 80% [26] .…”

Section: Introductionmentioning

confidence: 99%

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Diagnostik Antijen Olarak Rekombinant Sao-M Protein Kullanılarak Streptococcus suis’in Tanısı Amacıyla PPA-ELİSA Geliştirilmesi

Xia¹,

Zhang²,

Cheng³

et al. 2017

Kafkas Univ Vet Fak Derg

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Streptococcus suis, an important zoonotic agent, is responsible for outbreaks of human infections. The accurate and rapid detection of S. suis may help control the potential outbreak and ameliorate patient outcomes. In the present study, Sao-M was used to establish a horseradish peroxidase enzyme-linked staphylococcal protein A immunosorbent assay (PPA-ELISA) for the diagnosis of S. suis infection. Results of chessboard titration test showed that the optimal concentration of coating antigen and dilution of serum were 8 µg/ml and 1:80, respectively. The cut-off was confirmed as OD450≥0.351 for positive response. The specificity of test indicated that rSao-M had no cross-reaction with antisera against the other 6 species of pathogens. The variation coefficient of intra-batch and inter-batch in the repeating tests was less than 9.5%. Comparative analysis by using conventional ELISA kit and established GDH-based ELISA showed that the present PPA-ELISA has higher specificity and sensitivity than GDH-based ELISA. A total seroprevalence of 6.6% in 500 pig serum samples indicated the method's applicability to detect S. suis infection. Cumulatively, the results suggested that the PPA-ELISA is a rapid, sensitive and specific diagnostic method and could be used as a new tool for large-scale epidemiological surveys and serological diagnosis of S. suis infection. Keywords: Streptococcus suis, PPA-ELISA, Sao-M, Diagnosis Diagnostik Antijen Olarak Rekombinant Sao-M Protein KullanılarakStreptococcus suis'in Tanısı Amacıyla PPA-ELİSA Geliştirilmesi ÖzetStreptococcus suis, insanlarda enfeksiyona neden olan salgınlardan sorumlu önemli bir zoonotik ajandır. S. suis'in doğru ve hızlı bir şekilde tespit edilmesi enfeksiyona bağlı salgınları kontrol edebilir ve hastalığa bağlı etkileri azaltabilir. Bu çalışmada, Sao-M, S suis enfeksiyonunun teşhisi amacıyla peroksidaz enzim-bağlı stafilokokal protein A immün testi (PPA-ELİSA) için kullanılmıştır. Satranç tahtası titrasyon testinin sonuçları, kaplama antijeninin optimal konsantrasyonu ve serum sulandırılmasını sırasıyla 8 µg/mL ve 1:80 olarak göstermiştir. Pozitif cevap için eşik değeri OD450≥0.351 olarak belirlendi. Testin spesifitesi rSao-M'nin diğer 6 patojen türe karşı kullanılan antiserum ile çapraz reaksiyon vermediğini gösterdi. Tekrar testlerinde yığın içi ve yığınlar arası varyasyon katsayısı %9.5'ten az idi. Geleneksel ELİSA kiti ve kurulan GDH tabanlı ELİSA'nın karşılaştırmalı analizinde, mevcut PPA-ELİSA testinin GDH tabanlı ELİSA testinden daha yüksek spesifite ve sensitiviteye sahip olduğunu gösterdi. 500 domuz serumu örneğinde toplam seroprevalansın %6.6 olduğu ve bunun yöntemin S. suis enfeksiyonunun saptanmasında uygunluğunu gösterdi. Sonuçlar, PPA-ELİSA'nın hızlı, duyarlı ve spesifik bir tanı yöntemi olduğunu ve S. suis enfeksiyonunun serolojik tanısı için büyük kapsamlı epidemiyolojik araştırmalarda yeni bir araç olarak kullanılabilirliğini göstermektedir.

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Initial steps of the pathogenesis of the infection caused byStreptococcus suis: fighting against nonspecific defenses

et al. 2016

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Edited by Wilhelm JustInteractions between a bacterial pathogen and its potentially susceptible host are initiated with the colonization step. During respiratory/oral infection, the pathogens must compete with the normal microflora, resist defense mechanisms of the local mucosal immunity, and finally reach, adhere, and breach the mucosal epithelial cell barrier in order to induce invasive disease. This is the case during infection by the swine and zoonotic pathogen Streptococcus suis, which is able to counteract mucosal barriers to induce severe meningitis and sepsis in swine and in humans. The initial steps of the pathogenesis of S. suis infection has been a neglected area of research, overshadowed by studies on the systemic and central nervous phases of the disease. In this Review article, we provide for the first time, an exclusive focus on S. suis colonization and the potential mechanisms involved in S. suis establishment at the mucosa, as well as the mechanisms regulating mucosal barrier breakdown. The role of mucosal immunity is also addressed. Finally, we demystify the extensive list of putative adhesins and virulence factors reported to be involved in the initial steps of pathogenesis by S. suis.

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Immunization with Recombinant Sao Protein Confers Protection against Streptococcus suis Infection

Cited by 99 publications

References 47 publications

Antibody Response Specific to the Capsular Polysaccharide Is Impaired in Streptococcus suis Serotype 2-Infected Animals

Antibody Response Specific to the Capsular Polysaccharide Is Impaired in Streptococcus suis Serotype 2-Infected Animals

Diagnostik Antijen Olarak Rekombinant Sao-M Protein Kullanılarak Streptococcus suis’in Tanısı Amacıyla PPA-ELİSA Geliştirilmesi

Initial steps of the pathogenesis of the infection caused byStreptococcus suis: fighting against nonspecific defenses

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