Immuno-electron Microscopic Demonstration of Luminal Release of Serotonin from Enterochromaffin Cells of Rat Embryo.

Fujimiya, Mineko; Okumiya, Kiyohito; Maeda, Toshihiro

doi:10.1267/ahc.28.555

Cited by 10 publications

(7 citation statements)

References 18 publications

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“…Comparable ultrastructural changes in the density of the secretory granules of gastric antral G cells (Forssmann and Orci 1969;Forssmann et al 1980;Håkanson et al 1982;Track et al 1978) and D cells (Lamberts et al 1991) have been reported with, Immunoelectron microscopic study of the luminal release of serotonin from rat enterochromaffin cells induced by high intraluminal pressure however, no coincident demonstration of ultrastructural intracellular immunoreactivities. As shown in our previous studies, the morphological change of the secretory granules was not always consistent with the change of intracellular localization of GI hormones (Fujimiya et al 1995b;Okumiya et al 1996). In this study the labeling of immunogold demonstrates the intracellular localization of 5HT immunoreactivity within EC cells.…”

Section: Introductionsupporting

confidence: 51%

“…v lar localization of 5HT in embryonic EC cells; we observed protrusion of apical cell membranes and diffuse localization of immunogold particles in the cytoplasmic matrix (Fujimiya et al 1995b). Biochemical analysis also detected considerable amounts of 5HT in the lumen of embryonic gut (Fujimiya et al 1995b).…”

Section: Fig 5a-c A′-b′mentioning

confidence: 73%

“…Biochemical analysis also detected considerable amounts of 5HT in the lumen of embryonic gut (Fujimiya et al 1995b). The physiological role of luminally released 5HT has been proposed in previous studies.…”

Section: Fig 5a-c A′-b′mentioning

confidence: 94%

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Immunoelectron microscopic study of the luminal release of serotonin from rat enterochromaffin cells induced by high intraluminal pressure

Fujimiya

Okumiya²,

Kuwahara

1997

Histochemistry and Cell Biology

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Since definitive morphological studies showing the luminal release of serotonin have not been reported, we used a perfused system which allows physiological monitoring and biochemical as well as morphological evidence indicating release of serotonin from enterochromaffin cells. Isolated vascularly and luminally perfused rat duodenums exposed to 5-35 cmH2O of luminal pressure were measured for release of serotonin into the blood vessels and intestinal lumen. Immediately after raising the luminal pressure, the duodenum was fixed for immunoelectron microscopic localization of serotonin. Peristaltic contraction and serotonin content of the perfusates were continuously measured. The luminal release of serotonin increased with elevated intraluminal pressure, but the vascular release of serotonin was not altered. Tetrodotoxin had no effect on the pressure-stimulated luminal serotonin release. Enterochromaffin cells in control animals without increased luminal pressure contained immunogold-labeled secretory granules in the apical and basal cytoplasm. After intraluminal pressure increased, many apical secretory granules were no longer dense and immunogold particles were localized over the cytoplasmic matrix and microvilli. These findings indicate that luminal serotonin release is increased after raising the intraluminal pressure and serotonin, normally stored in the secretory granules of enterochromaffin cells, appears to be released into the cytoplasmic matrix and then diffuses or is transported into the intestinal lumen.

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Section: Introductionsupporting

confidence: 51%

Section: Fig 5a-c A′-b′mentioning

confidence: 73%

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Immunoelectron microscopic study of the luminal release of serotonin from rat enterochromaffin cells induced by high intraluminal pressure

Fujimiya

Okumiya²,

Kuwahara

1997

Histochemistry and Cell Biology

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“…Immunoelectron microscopic studies have shown that serotonin (Fujimiya et al 1995;Okumiya et al 1996a) and gastrin (Okumiya et al 1996b) may exist in a nongranular form in the supranuclear cytoplasm of EC and gastrin cells, respectively. Our findings of an association between the intracellular distribution of the immunoreactivity of serotonin and gastrin with CgB, particularly in the luminal parts of the cells, may suggest a functional role for CgB as a carrier protein, at least for serotonin and gastrin.…”

Section: Discussionmentioning

confidence: 99%

Complex Co-localization of Chromogranins and Neurohormones in the Human Gastrointestinal Tract

Portela-Gomes

Stridsberg

Johansson

et al. 1997

J Histochem Cytochem.

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SUMMARY Co-localization of chromogranin (Cg) A, B, and C has been studied in different neuroendocrine cell types in histologically normal mucosa from human gastrointestinal tract (corpus, antrum, duodenum, ileum, and colon) using single-, double-, and triple-immunofluorescence stainings. Virtually all enterochromaffin (EC) cells contained CgA, and those in the luminal two thirds of the antral mucosa and villi of small intestine often also contained CgB. A few EC cells in the duodenal crypts contained CgC. Most gastrin cells harbored both CgB and CgA, although rather more CgB than CgA, but some gastrin cells contained all three types, i.e., also CgC. Some CCK cells also contained all three chromogranins. Enteroglucagon cells in the duodenal villi contained CgA and some CgB. CgA (but not B or C) was found in some secretin, GIP, enteroglucagon/peptide YY, and neurotensin cells. A few somatostatin cells contained CgA but neither CgB nor CgC. CgA and C were found mainly in the basal cell region, whereas CgB occurred more diffusely throughout the cytoplasm. This varying distribution suggests that not all secretory granules contain CgA, or that CgB may occur in a nongranular form. The varying composition of the different chromogranins may reflect their complex functional roles in the widespread neuroendocrine system. (J Histochem Cytochem 45:815-822, 1997)

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“…The route by which gut peptides produced in the endocrine cells enter the duodenal lumen is not yet completely understood. Fujimiya et al (1995) showed that enterochromaffin cells actively release serotonin into the lumen of the small intestine, and the ratio of luminal content to tissue content is higher in embryonic and neonatal gut compared to the gut of adult rats. Okumiya et al (1996), in an immuno-electron microscopic study, demonstrated changes in the subcellular localisation of gastrin granules (migration from basal to apical regions) and gastrin release into the small intestinal lumen induced by carbachol.…”

Section: Bioactive Protein and Peptides In The Gastrointestinal Tractmentioning

confidence: 99%

Regulatory peptides in milk, food and in the gastrointestinal lumen of young animals and children

Zabielski¹

1998

J. Anim. Feed Sci.

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The present article reviews recent advances in our understanding of the regulatory role played by milk in the physiology of the neonate. Importance of milk bioactive proteins and peptides (BAPP) and their endogenous counterparts (hormones, gut regulatory peptides, growth factors) in the regulation of the development of the gastrointestinal tract (GIT) structure and function in the new-born is discussed. Mother's milk is often replaced with a milk formula that contains little or no BAPP. Although deprivation of milk BAPP is not lethal to the neonate, it affects GIT development processes, produces noticeable reduction of animal performance and lowers the resistance to GIT disorders. It is recommended that either the industrial processing is modified so as to avoid BAPP degradation or that the commercial milk formulas and weaning feeds should be supplemented with BAPP.

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Immuno-electron Microscopic Demonstration of Luminal Release of Serotonin from Enterochromaffin Cells of Rat Embryo.

Cited by 10 publications

References 18 publications

Immunoelectron microscopic study of the luminal release of serotonin from rat enterochromaffin cells induced by high intraluminal pressure

Immunoelectron microscopic study of the luminal release of serotonin from rat enterochromaffin cells induced by high intraluminal pressure

Complex Co-localization of Chromogranins and Neurohormones in the Human Gastrointestinal Tract

Regulatory peptides in milk, food and in the gastrointestinal lumen of young animals and children

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