Immuno-magnetic beads-based extraction-capillary zone electrophoresis-deep UV laser-induced fluorescence analysis of erythropoietin

Wang, Heye; Dou, Peng; Lü, Chenchen; Liu, Zhen

doi:10.1016/j.chroma.2012.02.017

Cited by 21 publications

(15 citation statements)

References 53 publications

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“…A very useful characteristic of MBs is their easy manipulation by using external permanent magnets, independently of the microfluidic process. One interesting technique is the use of MBs as a solid support for immunoextraction [24][25][26][27][28]. In this study, haptoglobin was immobilized on particle surfaces.…”

Section: Introductionmentioning

confidence: 99%

Utilization of magnetic nanobeads for analyzing haptoglobin in human plasma as a marker of Alzheimer’s disease by capillary electrophoretic immunoassay with laser-induced fluorescence detection

Wang

Yang

et al. 2015

Analytica Chimica Acta

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Section: Introductionmentioning

confidence: 99%

Utilization of magnetic nanobeads for analyzing haptoglobin in human plasma as a marker of Alzheimer’s disease by capillary electrophoretic immunoassay with laser-induced fluorescence detection

Wang

Yang

et al. 2015

Analytica Chimica Acta

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“…To improve the LODs for native fluorescence detection of erythropoietin (EPO), Wang et al utilized a magnetic bead-based extraction system for pre-concentration. Using this procedure, it was possible to obtain an LOD of 10 nM, two orders of magnitude lower than what was possible with UV absorbance at 214 nm [83]. …”

Section: Detection Methodsmentioning

confidence: 99%

Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis

2014

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The development of therapeutic proteins and peptides is an expensive and time-intensive process. Biologics, which have become a multi-billion dollar industry, are chemically complex products that require constant observation during each stage of development and production. Post-translational modifications along with chemical and physical degradation from oxidation, deamidation, and aggregation, lead to high levels of heterogeneity that affect drug quality and efficacy. The various separation modes of capillary electrophoresis (CE) are commonly utilized to perform quality control and assess protein heterogeneity. This review attempts to highlight the most recent developments and applications of CE separation techniques for the characterization of protein and peptide therapeutics by focusing on papers accepted for publication in the in the two-year period between January 2012 and December 2013. The separation principles and technological advances of CE, capillary gel electrophoresis, capillary isoelectric focusing, capillary electrochromatography and CE-mass spectrometry are discussed, along with exciting new applications of these techniques to relevant pharmaceutical issues. Also included is a small selection of papers on microchip electrophoresis to show the direction this field is moving with regards to the development of inexpensive and portable analysis systems for on-site, high-throughput analysis.

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“…They include elastin, flavin, and pyridoxine . A number of drug molecules also absorb DUV light due to aromatic rings in their structures, and emit fluorescence at DUV excitation . These molecules are potential targets of DUV autofluorescence imaging and analysis.…”

Section: Autofluorescence From Biomolecules At Duv Excitationmentioning

confidence: 99%

Deep‐Ultraviolet Biomolecular Imaging and Analysis

Kumamoto

Taguchi

Kawata

2018

Advanced Optical Materials

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Deep‐ultraviolet light, 200–300 nm in wavelength, interacts with nucleic acids and proteins strongly compared to visible and infrared light. In this article, the interaction between deep‐ultraviolet photons and biomolecules is discussed. Especially, the absorption and autofluorescence of biomolecules by the deep‐ultraviolet excitation are examined. Applications of deep‐ultraviolet absorption and autofluorescence to label‐free biomolecular imaging and analysis of cells and tissues are shown. Resonant Raman scattering at nucleotide bases and aromatic amino acids as another important topic of deep‐ultraviolet photonics is reviewed in biomolecular imaging and analysis. The discussion extends to the recent progress in the development of deep‐ultraviolet microscopes as well as a variety of deep‐ultraviolet optical devices such as light sources, detectors, and objectives. The issue of photodamage due to deep‐ultraviolet irradiation on cells and tissues is also discussed. It has been recently suppressed with use of lanthanide ions. The experimental results of the photodamage suppression are shown. For surface‐enhanced resonant Raman scattering, autofluorescence enhancement, and tip‐enhanced resonant Raman scattering microscopy, plasmonic materials applicable in the deep‐ultraviolet range are discussed.

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Immuno-magnetic beads-based extraction-capillary zone electrophoresis-deep UV laser-induced fluorescence analysis of erythropoietin

Cited by 21 publications

References 53 publications

Utilization of magnetic nanobeads for analyzing haptoglobin in human plasma as a marker of Alzheimer’s disease by capillary electrophoretic immunoassay with laser-induced fluorescence detection

Utilization of magnetic nanobeads for analyzing haptoglobin in human plasma as a marker of Alzheimer’s disease by capillary electrophoretic immunoassay with laser-induced fluorescence detection

Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis

Deep‐Ultraviolet Biomolecular Imaging and Analysis

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