Immuno-modulatory and Therapeutic Effect of Curcumin in an Allergensensitized Murine Model of Chronic Asthma

Paul, Pramathadhip; Majhi, Sourav; Mitra, Shinjini; Banerjee, Ena Ray

doi:10.4172/2155-9899.1000551

Cited by 3 publications

(6 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the CD45+B220+ are B cells, CD3+CD4+ are helper T (TH) cells, and neutrophils are CD45+Gr1+. All cells were examined by flow cytometry (Guava ® easyCyte, Merck Millipore, Mumbai, Maharashtra, India) and the results were analyzed using FCS Express (version 7 plus, De Novo Software, Pasadena, CA, USA) [ 13 , 14 ].…”

Section: Methods and Methodsmentioning

confidence: 99%

Non-Invasive Delivery of Nano-Emulsified Sesame Oil-Extract of Turmeric Attenuates Lung Inflammation

Rasool

Alavala²,

Kulandaivelu³

et al. 2020

Pharmaceutics

View full text Add to dashboard Cite

Turmeric, the golden Indian spice, and the edible oil of sesame seeds are the essential ingredients of Indian food created by ancestors and established the belief of the curative effect of food for many generations. Considering the anti-inflammatory effects of turmeric, we formulated a nano-emulsion of turmeric infused in edible sesame oil, with a globule size of 200–250 nm using high-energy microfluidization. The product with a zeta potential of −11.5 mV showed spherical globules when imaged for scanning and transmission electron microscopy. We explored the anti-inflammatory potential of this edible nano-emulsion in lung inflammation. The lungs are the internal organ most vulnerable to infection, injury, and rapid inflammation from the external environment because of their constant exposure to pollutants, pathogenic microorganisms, and viruses. We evaluated the nano-emulsion for efficacy in ovalbumin-induced lung injury in mice with an oral treatment for two weeks. The therapeutic effect of nano-emulsion of the sesame oil-extract of turmeric was evident from biochemical analysis of bronchoalveolar lavage fluid, lung histopathology, and flow cytometric analysis. The developed nano-emulsion significantly reduced the inflammation and damage to the alveolar network in ovalbumin-injured mice. Significant reduction in the levels of neutrophils and inflammatory cytokines like IL-4, IL-6, and IL-13 in bronchoalveolar lavage fluid was observed in the nano-emulsion-treated group. Leukotriene B4 and IgE were also significantly altered in the treated group, thus suggesting the suitability of the formulation for the treatment of allergy and other inflammatory conditions. The nano-emulsification process potentiated the immunoregulatory effect of turmeric, as observed from the elevated levels of the natural anti-inflammatory cytokine, IL-10. The dietary constituents-based nano-emulsion of spice turmeric helped in scavenging the free radicals in the injured lungs, thus modulating the inflammation pathway. This easily scalable formulation technology approach can therefore serve as a potential noninvasive and safe treatment modality for reducing lung inflammation in lung injury cases.

show abstract

Section: Methods and Methodsmentioning

confidence: 99%

Non-Invasive Delivery of Nano-Emulsified Sesame Oil-Extract of Turmeric Attenuates Lung Inflammation

Rasool

Alavala²,

Kulandaivelu³

et al. 2020

Pharmaceutics

View full text Add to dashboard Cite

show abstract

“…cDNA was prepared using reverse transcriptase III. Total mRNA was assessed by Reverse Transcriptase PCR (RT-PCR) and Real time PCR (qRT) as previously described 20 .…”

Section: Gene Expressionmentioning

confidence: 99%

“…Total mRNA were assessed by quantitative PCR (qPCR) analysis as previously mentioned 20 . The primers used for Real time PCR are listed in Table 1 A, with β -actin as the internal control.…”

Section: Real Time (Qrt)-pcrmentioning

confidence: 99%

“…mRNA expression of different cytokine genes were assessed by RT-PCR as previously described 20 . Gene specific primers are listed in Table 1B and GAPDH was taken as a house-keeping gene.…”

Section: Reverse Transcriptase (Rt)-pcrmentioning

confidence: 99%

“…The antibodies were rabbit anti-mouse NFκB (Santa Cruz), rabbit anti-mouse TGFβ , rabbit anti-mouse STAT6 (Santa Cruz) and rabbit anti-mouse GAPDH (Santa Cruz). Western blot protocol was described previously 20 .…”

Section: Analysis Of Protein Expression By Western Blotmentioning

confidence: 99%

See 2 more Smart Citations

Orally administered fisetin as an immuno-modulatory and therapeutic agent in a mouse model of chronic allergic airway disease

et al. 2019

Self Cite

View full text Add to dashboard Cite

Introduction: Allergic asthma is a prevalent disorder, in which eosinophilic inflammation is involved in the lungs. Asthma affects people all over the world, regardless of the country's level of development. Chronic allergen-induced fibrotic damage of the lungs is stimulated in 55 days, which results in significant tissue destruction constitutive to pulmonary tissues, in addition to extensive oxidative & inflammation-induced damage of small and large airways. To date, there is no cure for asthma, and symptoms are controlled using corticosteroids, which may cause systemic side effects. Flavonoids, like fisetin, are a class of secondary metabolites produced by plants, which are known to have numerous beneficial effects. Previous report demonstrated that fisetin has beneficial effects against various diseases such as cancers, tumors, diabetes, and alcohol-induced liver injury. Methods: In the present study, chronic allergic disease (asthma) was developed in C57BL/6J mice, using intraperitoneal injection of ovalbumin for 54 days together with orally administered fisetin as a treatment strategy. Fisetin was administered 1 hour before intratracheal treatment. On day 55, treated animals were sacrificed, and tissues were collected for various assays. Results: Fisetin was found to reduce the symptoms of asthma significantly. Reduction in total cell infiltration, eosinophil count, and the levels of serum IgE were observed. There was a down regulation in CD3+CD4+ TH cells, and a decrease in the deposition of collagen in the lung and airways. Conclusion: From these observations, we conclude that fisetin is effective in the treatment of asthma, and a pathway by which fisetin acts was hypothesized.

show abstract

Scientific rationale of Indian AYUSH Ministry advisory for COVID-19 prevention, prophylaxis, and immunomodulation

Gupta¹,

Sonewane²,

Rajan

et al. 2021

ADV TRADIT MED (ADTM)

Self Cite

View full text Add to dashboard Cite

The current outbreak of COVID-19 is caused by the SARS-CoV-2 virus that has affected > 210 countries. Various steps are taken by different countries to tackle the current war-like health situation. In India, the Ministry of AYUSH released a self-care advisory for immunomodulation measures during the COVID-19 and this review article discusses the detailed scientific rationale associated with this advisory. Authors have spotted and presented in-depth insight of advisory in terms of immunomodulatory, antiviral, antibacterial, co-morbidity associated actions, and their probable mechanism of action. Immunomodulatory actions of advised herbs with no significant adverse drug reaction/toxicity strongly support the extension of advisory for COVID-19 prevention, prophylaxis, mitigations, and rehabilitation capacities. This advisory also emphasized Dhyana (meditation) and Yogasanas as a holistic approach in enhancing immunity, mental health, and quality of life. The present review may open-up new meadows for research and can provide better conceptual leads for future researches in immunomodulation, antiviral-development, psychoneuroimmunology, especially for COVID-19.

show abstract

Immuno-modulatory and Therapeutic Effect of Curcumin in an Allergensensitized Murine Model of Chronic Asthma

Cited by 3 publications

References 22 publications

Non-Invasive Delivery of Nano-Emulsified Sesame Oil-Extract of Turmeric Attenuates Lung Inflammation

Non-Invasive Delivery of Nano-Emulsified Sesame Oil-Extract of Turmeric Attenuates Lung Inflammation

Orally administered fisetin as an immuno-modulatory and therapeutic agent in a mouse model of chronic allergic airway disease

Scientific rationale of Indian AYUSH Ministry advisory for COVID-19 prevention, prophylaxis, and immunomodulation

Contact Info

Product

Resources

About