Immuno‐Polymeric Nanoparticles by Diels–Alder Chemistry

Magnetic hyaluronic acid nanospheres via aqueous Diels–Alder chemistry to deliver dexamethasone for adipose tissue engineering

“…In the past few years, the use of aqueous Diels-Alder chemistry has been known in conjugation strategy in preparation of biomaterials [31,32]. For example, conjugation of biopolymers through aqueous Diels-Alder chemistry has been employed for preparation of biodegradable hydrogels for cell culture and drug delivery [33][34][35]. These successful results clearly illustrate that aqueous Diels-Alder chemistry promise as conjugation strategy for cell scaffolding materials.…”

Direct Synthesis of Biodegradable Polysaccharide Derivative Hydrogels Through Aqueous Diels‐Alder Chemistry

“…[36][37] 0.5 g HA sodium (MW %1.6 Â 10 6 ) was dissolved in 100 ml nanopure H 2 O and activated by N-ethyl-N 0 -(3-dimethylaminopropyl) carbodiimide hydrochloride/N-hydroxysulfosuccinimide (EDAC/Sulfo-NHS). 0.5 g Furan-PEG-NH 2 was dissolved in a borate buffer solution (500 mM, pH 9.0) and then slowly added to the activated HA sodium solution under stirring.…”

“…[29][30][31][32][33][34][35][36][37] Given the high specificity and efficiency, the success of aqueous Diels-Alder chemistry in biomolecule conjugation and immobilization may be extended to the synthesis of biodegradable hydrogels. To the best of our knowledge, the use of aqueous Diels-Alder chemistry strategy for biodegradable hydrogel fabrication has received limited attention.…”

“…The unreacted aldehyde sites were blocked by adding 1 ml of 1 M ethanolamine (pH 9.6). [37] The maleimide-functionalized HA derivative was obtained as a white solid by freeze-drying after exhaustive dialysis against nanopure water. Additional detail is available in Supplementary Fig.…”

Diels–Alder Cycloaddition in Protein Chemistry

“…Another interesting application was the preparation of immuno polymeric nanoparticles by chemoselective immobilization of antibodies. [47] Antibodies anti-HER2 (a therapeutic antibody used to treat breast cancer) functionalized with maleimide (44) in the carbohydrate chains within the Fc region were specifically incorporated on furan-functionalized micellar nanoparticles derivatized by Diels-Alder in aqueous solution (Scheme 15). This was efficient binding with breast cancer HER2-over-expressing cell lines in vitro.…”