Immuno-probed graphene nanoplatelets on electrolyte-gated field-effect transistor for stable cortisol quantification in serum

Nasyifa, Mohd Maidin Nur; Ruslinda, A. Rahim; Halim, Nur Hamidah Abdul; Abidin, Azrul Syafiq Zainol; Faudzi, Fatin Nabilah Mohd; Ahmad, Nurul Atiqah; Lockman, Zainovia; Rezek, Bohuslav; Kromka, Alexander; Gopinath, Subash C.B.

doi:10.1016/j.jtice.2020.12.008

Cited by 20 publications

(20 citation statements)

References 36 publications

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“…Further, when albumin and globulin were dropped on aptamer‐modified surfaces instead of LH, there was no current response recorded (Figure 5B). Globulin (2.6–3.5 g/dl) and albumin (3.5–5.0 g/dl) are the major and predominant proteins in the blood serum with the molecular sizes of ∼150 and 66.5 kDa, respectively 38 . Present study is embarked to demonstrate the genuine interaction between aptamer and LH in the presence of these proteins, which supports the condition with the real sample analysis.…”

Section: Resultssupporting

confidence: 79%

“…Globulin (2.6-3.5 g/dl) and albumin (3.5-5.0 g/dl) are the major and predominant proteins in blood serum with the molecular sizes of ∼150 and 66.5 kDa, respectively. 38 Present study is embarked to demonstrate the genuine interaction between aptamer and LH in the presence of these proteins, which supports the condition with the real sample analysis. These overall results confirm that our aptamer-modified sensing electrode surface did not show any biofouling effect and it only allows the specific interaction of LH with aptamer.…”

Section: Biofouling Of Control Molecules On Go-aptamer-modified Elect...supporting

confidence: 73%

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Aptasensing luteinizing hormone to determine gynecological endocrine complications on graphene oxide layered sensor

Sun¹,

Bao

Yao

et al. 2021

Biotech and App Biochem

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Luteinizing hormone (LH)/lutropin is an interstitial cell-stimulating hormone playing a predominant role in the reproductive system, and highly correlated with the infertility treatment in both men and women. This research was concentrated to quantify LH level by using interdigitated electrode sensor. To improve the electric current flow, sensing electrode was modified with graphene oxide (GO) and the aptamer probe was attached on GO through biotin-streptavidin linker. Current responses were measured with aptamer-LH interaction at the target concentrations between 7.5 nM and 1 µM and the detection limit of LH was calculated as 60 nM with the determination coefficient (R 2 ) value, 0.9229 [y = 1.296x − 2.8435] on a linear range from 30 nM to 1 µM. Further, biofouling effect on sensing electrode surface was analyzed with complementary aptamer sequence, control proteins (albumin and globulin). The above GO-aptamermodified interdigitated electrode sensor helps to quantify LH level and diagnose gynecological endocrinology-related complications.

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Section: Resultssupporting

confidence: 79%

Section: Biofouling Of Control Molecules On Go-aptamer-modified Elect...supporting

confidence: 73%

Aptasensing luteinizing hormone to determine gynecological endocrine complications on graphene oxide layered sensor

Sun¹,

Bao

Yao

et al. 2021

Biotech and App Biochem

View full text Add to dashboard Cite

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“…The sensor is limited by the adsorption kinetics of MIP as evident by logarithmic sensitivity compared to linear sensitivity offered by some sensors reported in Tables S1 and S2. While the prepared sensor exhibits better sensitivity compared to other PPy based MIP, it falls short compared to some reported cortisol immunosensor [72 μA log –1 (g/mL)] . One of the future endeavors is to leverage computational tools which can help in characterization and optimization of materials and methods to enhance sensitivity and performance of the sensor …”

Section: Resultsmentioning

confidence: 97%

“…While the prepared sensor exhibits better sensitivity compared to other PPy based MIP, 17 it falls short compared to some reported cortisol immunosensor [72 μA log −1 (g/mL)]. 27 One of the future endeavors is to leverage computational tools which can help in characterization and optimization of materials and methods to enhance sensitivity and performance of the sensor. 9 The response of the MIP can be fitted to an adsorption isotherm to understand the affinity of MIP toward the target.…”

Section: Analytical Performancementioning

confidence: 94%

“…The combination of β-CD functioning as complimentary recognition sites in conjunction with MIP, which increases the template imprinting during MIP fabrication . Graphene and its derivatives have been extensively studied for electrochemical biosensing applications, in conjunction with antibodies as immunosensors , and aptamers . Graphene oxide (GO) is an attractive interface material which can be produced inexpensively by wet oxidation and exfoliation of graphite in comparison to traditional electrochemical electrodes.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Redox-Label-Doped Molecularly Imprinted Polymer on β-Cyclodextrin/Reduced Graphene Oxide for Electrochemical Detection of a Stress Biomarker

Goyal

Sakata

2022

ACS Omega

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Cortisol is a major stress biomarker involved in the regulation of metabolic and immune responses. Readily accessible assays with sufficient quantitative and temporal resolution can assist in prevention, early diagnosis, and management of chronic diseases. Whereas conventional assays are costly in terms of time, labor, and capital, an electrochemical approach offers the possibility of miniaturization and detection at the point-of-care. Here, we investigate the biosensor application of molecularly imprinted polypyrrole (PPy) doped with hexacyanoferrate (HCF) and coupled to reduced graphene oxide functionalized with β-cyclodextrin (β-CD). β-CD provides an inclusion site for lipophilic cortisol and was electrochemically grafted simultaneous with reduction of GO. Next, PPy was electrochemically deposited in presence of cortisol template with HCF dopant ions serving as intrinsic redox probe. Thus, the sensor response was evaluated via changes of redox peak current in cyclic voltammetry and demonstrated a broad logarithmic detection range (5 pg/mL to 5000 ng/mL, R 2 = 0.995), with a sensitivity of 8.809 μA log –1 (ng/mL) cm –2 and LOD of 19.3 pM. The sensor was shown to be specific toward cortisol in reference to salivary cortisol concentration in saliva over structural analogues. The sensor was exhibited to determine cortisol in artificial saliva at normal and elevated levels. The good performance and facile electrochemical fabrication of this antibody- and external label-free interface are promising for the development of affordable point-of-care biosensors.

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Electrochemical Affinity Sensors Using Field Effect Transducer Devices for Chemical Analysis

2021

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The review presents advances and main challenges of the affinity sensors based on field-effect transistors published during the last five years. The different nanomaterial-based field-effect transistors are classified according to the nature of the nanomaterials, beginning by silicon, the "gold-standard" semiconductor, the gallium nitride semiconductor, the organic semiconductors, the silicon nanowires, the inorganic nanomaterials, the carbon nanotubes and the graphene. Due to its exceptional electrical properties, the main works are devoted to graphene. The obtained analytical performances for the detection of biomarkers, of DNA sequences and of miRNA are listed. The relation between the operational conditions -nature of the nanomaterials, procedure of preparation, choice of the receptor molecule, method of immobilization -and the analytical performance are discussed. The perspective of industrialization of these affinity sensors based on field-effect transistors is discussed.

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Immuno-probed graphene nanoplatelets on electrolyte-gated field-effect transistor for stable cortisol quantification in serum

Cited by 20 publications

References 36 publications

Aptasensing luteinizing hormone to determine gynecological endocrine complications on graphene oxide layered sensor

Aptasensing luteinizing hormone to determine gynecological endocrine complications on graphene oxide layered sensor

Development of a Redox-Label-Doped Molecularly Imprinted Polymer on β-Cyclodextrin/Reduced Graphene Oxide for Electrochemical Detection of a Stress Biomarker

Electrochemical Affinity Sensors Using Field Effect Transducer Devices for Chemical Analysis

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