Immuno-rolling circle amplification using a multibinding fusion protein

DNA for Non-nucleic Acid Sensing

“…2. The strategy is to extend the nucleotide sequence attached to the secondary antibody and to add a complementary DNA strand, often labelled by a fluorophore, after the rinsing steps (Akter et al 2011(Akter et al , 2012. Most systems work in a heterogeneous pattern particularly suitable for electrochemical detection.…”

Expanding Possibilities of Rolling Circle Amplification as a Biosensing Platform

“…20 Therefore, a series of treatments including precircularization and successive degradation of the linear ssDNA probe have dramatically improved the reliability of the RCA reaction due to clear negative control. [19][20][21][22][23] A lack of nonspecific amplification in test samples considerably contributes to the accuracy of RCA.…”

DNA-based immunoassays for sensitive detection of protein

“…Recently, we have developed a fusion product of the C2 domain of protein G with a biotin acceptor peptide (BAP) to make a bridge that links antigen-bound IgG on one side to a biotinylated short-primer DNA-bound streptavidin at the other end for IRCA development (Fig. 6a-ii) [1]. This system was able to detect ␣-fetoprotein in buffer at concentrations 100-fold lower than were detected by ELISA, and it eliminated the need for chemical conjugation.…”

“…The ultrasensitive detection of biomarkers in human body fluids is essential for early detection, treatment, and management of diseases [1], because even only a few protein molecules can be sufficient to affect the biological functions of cells and trigger pathophysiological processes. Throughout history, immunoassays have played an enormous role in disease diagnosis and in determining effective treatments for infectious diseases.…”