Immunoadjuvant activities of a recombinant chicken IL-12 in chickens vaccinated with Newcastle disease virus recombinant HN protein

Su, Bor Sheu; Yin, Hao; Chiu, Hua Hsien; Hung, Li Hsiang; Huang, Ji Ping; Shien, Jui Hung; Lee, Long Huw

doi:10.1016/j.vetmic.2011.02.047

Cited by 12 publications

(12 citation statements)

References 45 publications

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“…The induction of higher levels of humoral antibody responses after administration of IBDV/TIR-TLR7 may be in accordance with the ability of TLR7 in stimulation of B cells. Consistent with this, numerous studies have shown that immune stimulator factors such as chIL-18, chIFN-α and chIL-12 modulate both CMI and humoral immunity in chickens when co-administrated with an avian vaccine [19][20][21][22]. Functionally, the co-stimulatory molecules for DCs could stimulate B cells differentiation, proliferation, maturation and maintenance and they could also stimulate T cell development, proliferation and Fig.…”

Section: Discussionsupporting

confidence: 54%

“…homeostatic regulation. For example, it has been shown that ChIL-18 could increase CD4+ and CD8+ T cell populations, leading to production of IFN-γ, that is critical to CMI and IL-4 that enhances antibody production in chickens following coimmunization with NDV vaccine [19][20][21][22].The synergistic effects of IL-12 and IL-18 have been shown to result in effective differentiation of Th1 cells. It has been suggested that the deriving appropriate immune responses can be due to effective DCs stimulation to secrete IL-12 and IFN-γ [23].…”

Section: Discussionmentioning

confidence: 99%

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Enhancement of cell-mediated immune response in chickens by combination of TIR-TLR7 with inactivated Newcastle disease vaccine

Rashid

Shahsavandi

Ebrahimi

et al. 2018

vacres

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Introduction: Live and inactivated vaccines are wildly used against Newcastle disease (ND) which is a highly contagious and acute viral infection of domestic and wild birds. A higher and prolonged immune response is required to improve the control of the disease. The aim of this study was to evaluate the potential of the conserved TIR domain of an immune regulatory protein TLR7 (i.e. TIR-TLR7) as a biological adjuvant in enhancing cell-mediated immunity in vaccinated chickens against the inactivated ND virus (NDV) V4 strain antigen. Methods: NDV V4 strain was propagated in chicken embryonated SPF eggs, tittered and then inactivated by formalin. The amount of 10 μg of TIR-TLR7 was mixed with the NDV antigen before intramuscular administration. Fifty SPF chickens were divided in A-E groups (n=10), consisted of negative control, TIR-TLR7, inactivated NDV antigen, TIR-TLR7/inactivated NDV antigen in prime, and the same regimen in boost platform. The blood samples were collected at week intervals up to 6 weeks post-vaccination. Humoral response was measured by detection of specific NDV antibody titer using the HI test. The cell-mediated immunity was evaluated by measuring lymphocyte proliferation in splenocytes cell culture using MTT. Results: All immunized chickens with TIR-TLR7/inactivated NDV antigen had significant (P < 0.05) cellmediated and HI responses to NDV compared to the control groups. No statistically-significant difference was observed between the prime and boost trials. Conclusion: The results indicated that the combination of TIR-TLR7 and inactivated NDV antigen gave a strong immune response at both the humoral and the cellular levels.

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Enhancement of cell-mediated immune response in chickens by combination of TIR-TLR7 with inactivated Newcastle disease vaccine

Rashid

Shahsavandi

Ebrahimi

et al. 2018

vacres

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“…Viral shedding was not measured in either of the above-mentioned studies, and therefore, the impact of these approaches on shedding is not known. Recently, another poxvirus-based DNA vaccine targeting the HN protein and including IL-12 as an adjuvant was evaluated [30]. It was shown that IL-12 can enhance a cell-mediated immune response.…”

Section: Discussionmentioning

confidence: 99%

Immune effect of a Newcastle disease virus DNA vaccine with IL-12 as a molecular adjuvant delivered by electroporation

Xie

et al. 2020

Arch Virol

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Newcastle disease (ND), caused by virulent Newcastle disease virus (NDV) strains, has been one of the most problematic diseases affecting the poultry industry worldwide. Conventional vaccines provide effective protection for birds to survive ND outbreaks, but they may not completely suppress NDV shedding. NDV strains circulate on farms for a long time after the initial infection and cause potential risks. A new vaccine with fast clearance ability and low viral shedding is needed. In this study, we used interleukin-12 (IL-12) as an adjuvant and electroporation (EP) as an advanced delivery system to improve a DNA vaccine candidate. The fusion (F) protein gene from an NDV strain of the prevalent genotype VII.1.1 was cloned to prepare the vaccine. Chickens immunized with the F gene DNA vaccine co-delivered with an IL-12-expressing plasmid DNA showed higher neutralizing antibody levels and stronger concanavalin-A-induced lymphocyte proliferation than those treated with the F gene DNA vaccine alone. The co-delivered vaccine provided 100% protection, and less viral shedding and a shorter release time were observed in challenged chickens than when the F gene DNA vaccine was administered alone. The use of F gene DNA combined with IL-12 delivered by electroporation is a promising approach for vaccination against ND. Abbreviations APC Antigen-presenting cell CEF Chicken embryo fibroblast chIL-12 Chicken IL-12 DMEM Dulbecco's modified Eagle'

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“…HN is one of the membrane proteins of NDV and responsible for the recognition and absorption of the virus to sialic acid-containing receptors on the target cells. Humoral immune responses against the HN protein are able to protect chickens from NDV infection, while reports have indicated that HN would be a good target for vaccine development [ 43 – 45 ]. Previous research looking to characterize the antigenic region on the HN protein of NDV have used monoclonal antibodies derived from mice [ 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis and Characterization of Linear Antigenic Domains on HN Protein from Genotype VII Newcastle Disease Virus Using Yeast Surface Display System

et al. 2015

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Circulation of genotype VII Newcastle disease virus (NDV) has posed a great threat for the poultry industry worldwide. Antibodies against Hemagglutinin-neuraminidase (HN), a membrane protein of NDV with critical roles in NDV infection, have been reported to provide chickens protection from NDV infection. In this study, we comprehensively analyzed the in vivo antibody responses against the linear antigenic domains of the HN protein from genotype VII NDV using a yeast surface display system. The results revealed four distinct regions of HN, P1 (1-52aa), P2 (53-192aa), P3 (193-302aa) and P4 (303-571aa), respectively, according to their antigenic potency. Analysis by FACS and ELISA assay indicated P2 to be the dominant linear antigenic domain, with the immunogenic potency to protect the majority of chickens from NDV challenge. In contrast, the P1, P3 and P4 domains showed weak antigenicity in vivo and could not protect chickens from NDV challenge. These results provide important insight into the characteristic of humoral immune responses elicited by HN of NDV in vivo.

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Immunoadjuvant activities of a recombinant chicken IL-12 in chickens vaccinated with Newcastle disease virus recombinant HN protein

Cited by 12 publications

References 45 publications

Enhancement of cell-mediated immune response in chickens by combination of TIR-TLR7 with inactivated Newcastle disease vaccine

Enhancement of cell-mediated immune response in chickens by combination of TIR-TLR7 with inactivated Newcastle disease vaccine

Immune effect of a Newcastle disease virus DNA vaccine with IL-12 as a molecular adjuvant delivered by electroporation

Comprehensive Analysis and Characterization of Linear Antigenic Domains on HN Protein from Genotype VII Newcastle Disease Virus Using Yeast Surface Display System

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