Immunoanalytical Method for Quality Control of Orange Juice Products

Abstract

The main goal of the present study is to develop an immunoanalytical method for the quality control of orange juice products. Peptides from various parts (juice, albedo, and flavedo) of citrus fruits (orange, mandarin, grapefruit, and lemon) were analyzed and isolated by SDS-PAGE. Antisera were developed in mice against the protein pool of orange juice and peel and tested by Western blot analysis. Using these antisera, some juice- and peel-specific peptides were detected. One of the antibodies in the antiserum developed against peel proteins recognized a single peel-specific peptide with a molecular mass of 28 kDa in 10000-fold dilution. It did not give any positive reactions against the sample prepared from the juice. The 24 and 27 kDa juice-specific peptides were isolated in electrophoretically pure form, and polyclonal antibodies were developed against them in mice. The anti-27 kDa antibody reacted with a 29 kDa protein in the peel sample, and it gave a positive reaction against the 27 kDa peptide of the juice. The antibodies developed in the course of the present work seem to be useful for determining the juice content in commercial citrus beverages and for evaluating the peel contamination in them.

Characterization of blond and Star Ruby (red) Jaffa grapefruits using antioxidant and electrophoretic methods

“…Both forms of grapefruits showed small differences in their protein patterns. Our results confirm the data of other investigators (Sass-Kiss & Sass, 2000.…”

“…The main bands displayed by SDS-PAGE were between 20 and 43 kDa. The results were in agreement with others (Sass-Kiss & Sass, 2000), but in their case the proteins were precipitated with trichloroacetic acid (TCA) and then separated with SDS-PAGE. In the present report the proteins were preliminary not precipitated, but the method was modified using different gel concentrations.…”

“…Resolution of the bands was satisfactory in the molecular weight region (>36 kDa), but poor in the low molecular weight region (<20 kDa). Nevertheless, we detected more bands in the <28 kDa zone than Sass-Kiss & Sass, 2000. The use of a longer gel (14 cm instead of 7 cm) gave more distinct and sharp bands, especially in the >36 kDa zone.…”

“…Entirely new possibilities appeared with the development of electrophoretic techniques (Firon et al, 1979;Sass-Kiss & Sass, 2000. As mentioned, interest in the characterization of citrus fruits is growing, however investigations of the cultivars of the same citrus fruits are practically non-existent (Gorinstein et al, 2001a;Proteggente et al, 2002;Sun et al, 2002;Gorinstein et al, 2003;Sass-Kiss et al, 2004).…”

Fouling Analysis and the Recovery of Phytosterols from Orange Juice Using Regenerated Cellulose Ultrafiltration Membranes

“…Orange juice contains a polydisperse distribution of particle sizes from pulp trashes to small particles less than 2 μm in diameter (Corredig et al 2001;Stinco et al 2012). In addition, the molecular weight of proteins in orange juice was 12 to 71 kDa (Sass-Kiss and Sass 2000). No literature is available on the performance of ultrafiltration processes for the separation of phytosterols from proteins in fruit juices.…”