Immunoassay-based measurement of clinical biomarkers for monitoring changes in nasal cavity

Zhang, Yan J; Luroe, Sherri; Schieber, Frank C; Kelsey, Joan; Nabbie, Fizal; Rizzi, Giovanni; Richards, Penny; Weiner, Russell; Rhyne, Paul

doi:10.1016/j.jpba.2009.06.043

Cited by 10 publications

(9 citation statements)

References 41 publications

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“…In the present study MPO and IL-8 concentrations in NL-fluid correlated with high significance for all assessed groups. This accords to our expectations, as both IL-8 and MPO are markers of neutrophil activity [15,20].…”

Section: Correlation Between Mpo and Il-8 As Well As Pathogen Colonizsupporting

confidence: 92%

“…This finding is new, as to our knowledge, MPO has not been assessed previously in NL from CF patients. MPO is a neutrophil-derived protein released during oxidative burst and catalyzes the production of various oxidants, so that elevated levels can be regarded as markers for neutrophil activity [20,21]. Previously, in CF elevated MPO concentrations were found in serum [22], sputum [23][24][25], and BAL compared to healthy controls [21,26].…”

Section: Mpomentioning

confidence: 99%

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Soluble inflammation markers in nasal lavage from CF patients and healthy controls

Beiersdorf

Schien

Hentschel

et al. 2013

Journal of Cystic Fibrosis

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Section: Correlation Between Mpo and Il-8 As Well As Pathogen Colonizsupporting

confidence: 92%

Section: Mpomentioning

confidence: 99%

Soluble inflammation markers in nasal lavage from CF patients and healthy controls

Beiersdorf

Schien

Hentschel

et al. 2013

Journal of Cystic Fibrosis

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“…Myeloperoxidase (MPO) is produced by stimulated neutrophils and catalyses the production of various oxidants [20,21]. Elevated MPO is an established marker for neutrophil activity as it is released in oxidative bursts.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of soluble and cellular inflammatory markers in nasal lavage obtained from Cystic Fibrosis patients during intravenous antibiotic treatment

et al. 2014

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BackgroundIn cystic fibrosis (CF) patients, the upper airways display the same ion channel defect as evident in the lungs, resulting in chronic inflammation and infection. Recognition of the sinonasal area as a site of first and persistent infection with pathogens, such as Pseudomonas aeruginosa, reinforces the “one-airway” hypothesis. Therefore, we assessed the effect of systemic antibiotics against pulmonary pathogens on sinonasal inflammation.MethodsNasal lavage fluid (NLF) from 17 CF patients was longitudinally collected prior to and during elective intravenous (i.v.) antibiotic treatment to reduce pathogen burden and resulting inflammation (median treatment time at time of analysis: 6 days). Samples were assessed microbiologically and cytologically. Cytokine and chemokine expression was measured by Cytometric Bead Array and ELISA (interleukin (IL)-1β, IL-6, IL-8, MPO, MMP9, RANTES and NE). Findings were compared with inflammatory markers from NLF obtained from 52 healthy controls.ResultsInitially, the total cell count of the NLF was significantly higher in CF patients than in controls. However after i.v. antibiotic treatment it decreased to a normal level. Compared with controls, detection frequencies and absolute concentrations of MPO, IL-8, IL-6 and IL-1β were also significantly higher in CF patients. The detection frequency of TNF was also higher. Furthermore, during i.v. therapy sinonasal concentrations of IL-6 decreased significantly (P = 0.0059), while RANTES and MMP9 levels decreased 10-fold and two-fold, respectively. PMN-Elastase, assessed for the first time in NFL, did not change during therapy.ConclusionsAnalysis of NLF inflammatory markers revealed considerable differences between controls and CF patients, with significant changes during systemic i.v. AB treatment within just 6 days. Thus, our data support further investigation into the collection of samples from the epithelial surface of the upper airways by nasal lavage as a potential diagnostic and research tool.

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“…Of particular interest is the study of multiple potential biomarkers to identify predictors of disease progression or outcome (12,13,28). A widely used approach for relating systemic inflammation or immune status to disease outcome is the measurement of soluble biomarkers in blood (2,26), tissue culture supernatant (24), or mucosal secretions (32). Historically, studies have focused on quantitation of one or a few soluble biomarkers for correlation with or prediction of disease outcome.…”

mentioning

confidence: 99%

Multisite Comparison of High-Sensitivity Multiplex Cytokine Assays

Breen

Reynolds

Cox

et al. 2011

Clin Vaccine Immunol

152

130

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The concentrations of cytokines in human serum and plasma can provide valuable information about in vivo immune status, but low concentrations often require high-sensitivity assays to permit detection. The recent development of multiplex assays, which can measure multiple cytokines in one small sample, holds great promise, especially for studies in which limited volumes of stored serum or plasma are available. Four high-sensitivity cytokine multiplex assays on a Luminex (Bio-Rad, BioSource, Linco) or electrochemiluminescence (Meso Scale Discovery) platform were evaluated for their ability to detect circulating concentrations of 13 cytokines, as well as for laboratory and lot variability. Assays were performed in six different laboratories utilizing archived serum from HIV-uninfected and -infected subjects from the Multicenter AIDS Cohort Study (MACS) and the Women's Interagency HIV Study (WIHS) and commercial plasma samples spanning initial HIV viremia. In a majority of serum samples, interleukin-6 (IL-6), IL-8, IL-10, and tumor necrosis factor alpha were detectable with at least three kits, while IL-1␤ was clearly detected with only one kit. No single multiplex panel detected all cytokines, and there were highly significant differences (P < 0.001) between laboratories and/or lots with all kits. Nevertheless, the kits generally detected similar patterns of cytokine perturbation during primary HIV viremia. This multisite comparison suggests that current multiplex assays vary in their ability to measure serum and/or plasma concentrations of cytokines and may not be sufficiently reproducible for repeated determinations over a long-term study or in multiple laboratories but may be useful for longitudinal studies in which relative, rather than absolute, changes in cytokines are important.

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Immunoassay-based measurement of clinical biomarkers for monitoring changes in nasal cavity

Cited by 10 publications

References 41 publications

Soluble inflammation markers in nasal lavage from CF patients and healthy controls

Soluble inflammation markers in nasal lavage from CF patients and healthy controls

Dynamics of soluble and cellular inflammatory markers in nasal lavage obtained from Cystic Fibrosis patients during intravenous antibiotic treatment

Multisite Comparison of High-Sensitivity Multiplex Cytokine Assays

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