Immunoassay for acetamiprid detection: application to residue analysis and comparison with liquid chromatography

Abstract

This work describes the fundamental ability of a commercial ELISA to determine acetamiprid and the application of the ELISA to residue analysis in fruit and vegetable samples. The ELISA exhibited satisfactory sensitivity (I (50) 0.6 ng/g; limit of detection 0.053 ng/g) and a high selectivity for acetamiprid versus other neonicotinoid analogs (thiacloprid amide). Methanol, which influenced the sensitivity of the ELISA the least, was selected as the extractant for the ELISA analysis. Simple dilution of sample extracts with water eliminated matrix interferences. Average recoveries from the acetamiprid-spiked agricultural samples were >95% using a simple extraction method. Analytical results obtained from the ELISA were comparable to those obtained from the reference HPLC method (r>0.99). The ELISA applied to the residue analysis of acetamiprid in agricultural products is a rapid, simple, and cost-effective method, and could be successfully applied to the detection of acetamiprid before the distribution of produce.

Review on Current Analytical Methods with Chromatographic and Nonchromatographic Techniques for New Generation Insecticide Neonicotinoids

“…Table 3 shows that the easiest method to avoid the matrix effect is dilution of the sample extract (mainly methanol extract) with water or phosphate buffer. The recovery from various measurement samples were generally good in all reports (Byrne et al, 2005;Eisenback et al, 2009;Kim et al, 2006;Ma et al, 2009;Watanabe et al, 2001;Watanabe et al, 2004aWatanabe et al, , 2004bWatanabe et al, , 2006Watanabe et al, , 2011Xu et al, 2010). The measurement sensitivity of ELISA is apt to be affected by the concentration of extraction solvent (mainly methanol) coexisting in the sample solution during measurement (Nunes et al, 1998).…”

“…can be used easily for monitoring tests of a specific neonicotinoid insecticide. However, it is important in the use of kit-based ELISA to remember that the matrix effect should be evaluated in advance (Byrne et al, 2005;Watanabe et al, 2004aWatanabe et al, , 2004bWatanabe et al, , 2006Watanabe et al, , 2011. In any event, when ELISA is applied to a sample, it might be affected by the matrix effect.…”

Dispersive Solid-Phase Extraction Clean-up Combined with Dispersive Liquid–Liquid Microextraction for the Determination of Neonicotinoid Insecticides in Vegetable Samples by High-Performance Liquid Chromatography

“…They are mainly determined by highperformance liquid chromatography (HPLC) coupled to either mass spectrometry (Muccio et al 2006;Fidente et al 2005;Seccia et al 2005;Obana et al 2003) or diode array detection (DAD) (Seccia et al 2008;Obana et al 2002;Watanabe et al 2007;Fernández-Alba et al 1996). On the other hand, enzyme-linked immunosorbent assays has also been explored as a rapid and simple screening method for the compounds (Watanabe et al 2004;Watanabe et al 2006).…”

“…Liquid-liquid extraction (LLE) (Fernández-Alba et al 1996;Watanabe et al 2004;Watanabe et al 2006;Banerjee et al 2007) and solidphase extraction (SPE) (Muccio et al 2006;Fidente et al 2005;Seccia et al 2005;Obana et al 2003;Seccia et al 2008;Obana et al 2002;Watanabe et al 2007) are the most commonly used techniques for the preconcentration and clean-up of the neonicotinoid insecticides. However, LLE suffers from the disadvantage of requiring both large amount of samples and toxic organic solvents.…”

Aptamer-based Resonance Light Scattering for Sensitive Detection of Acetamiprid

“…Despite that it is considered to have relatively low acute and chronic mammalian toxicity, due to its frequent and extensive usage, there is probability to cause potential risk of human health. 1 At present, there are many methods used to detect acetamiprid, for instance, high-performance liquid chromatography (HPLC), [2][3][4] enzyme-linked immunosorbent assay (ELISA) 5,6 and liquid chromatography (LC) [7][8][9][10] etc. All of these methods have many advantages; however, they also encounter some disadvantages, for example: HPLC and LC suffer from complicated sample pretreatment, time consuming and inappropriate on-site operation.…”