Immunoassay for parathion without its prior removal from solution in hexane

Francis, John M.; Craston, Derek H.

doi:10.1039/an9941901801

Cited by 21 publications

(6 citation statements)

References 14 publications

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“…An alternative immuno-assay strategy for the quantification of hydrophobic analytes has exploited the use of reverse miscelles [21][22][23]. The antibody is solubilised within an aqueous phase that is encapsulated by a suitable surfactant film, such that it forms a series of discrete pockets within a surrounding non-aqueous solvent, thereby eliminating antibody interaction with the organic-phase.…”

Section: Antibodies In the Organic-phasementioning

confidence: 99%

“…The antibody is solubilised within an aqueous phase that is encapsulated by a suitable surfactant film, such that it forms a series of discrete pockets within a surrounding non-aqueous solvent, thereby eliminating antibody interaction with the organic-phase. Francis and Craston [21] describe an immunoassay for parathion in which the analyte is dissolved in n-hexane and interacts with polyclonal antibody within an aqueous miscelle, via diffusion across the phase boundary. However, the hexane-based immunoassay was 10 4 times less sensitive than the equivalent aqueous-phase assay.…”

Section: Antibodies In the Organic-phasementioning

confidence: 99%

See 1 more Smart Citation

Organic phase immunosensors

Setford¹,

Kröger²,

Turner³

1999

Analusis

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Many analytical methods involving non-polar analytes require solvent extraction prior to measurement. The analysis procedure is greatly simplified if the method is able to function effectively in the more non-polar solvent extract. This consideration, coupled with the increasing need for simple, specific and rapid diagnostic and screening tools, has focused interest in the development of organic-phase immunosensors.

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Section: Antibodies In the Organic-phasementioning

confidence: 99%

Section: Antibodies In the Organic-phasementioning

confidence: 99%

Organic phase immunosensors

Setford¹,

Kröger²,

Turner³

1999

Analusis

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“…In comparison to the monoclonal antibody-based nitrofluoranthene ELISA found in the literature (27), the present assay is almost three orders of magnitude more sensitive to 1-NP and shows far less cross-reactivity with parent PAHs and NPAHs. Preliminary investigations with crude airborne particulate extracts exhibited about 600% overestimation of 1-NP by ELISA compared to HPLC.…”

Section: Discussionmentioning

confidence: 83%

“…Despite the strong positive effect of MeCN on the ODs the assay detection limit was significantly decreased. Since the first report on the behavior of antibodies in anhydrous organic solvents (26), related investigations are of growing interest (27,28). It is known that antibody-antigen binding is dependent on noncovalent interactions, such as hydrophobic interactions, van der Waals forces, and hydrogen bonds.…”

Section: -(6-nitropyrenyl-l)-butyric Acid-klh 4-(8-nitropyrenyl-l)-bmentioning

confidence: 99%

Development of an Enzyme-Linked Immunosorbent Assay for 1-Nitropyrene

Knopp

Väänänen

Zühlke

1997

ACS Symposium Series

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An enzyme-linked immunosorbent assay (ELISA) was developed for 1-nitropyrene. For hapten syntheses pyrenebutyric acid was nitrated yielding a mixture of 4-(6-nitropyrene-1-y1)-and 4-(8-nitropyrene-1 -y1)-butyric acid isomers that could not be separated. High titered antisera were obtained in rabbits after immunization with a KLH immunogen prepared with the mixture of isomers. As coating antigen a corresponding BSA conjugate was used. The calibration curve was linear in the 0.1 to 10 ppb range with a center point at 1.5 ppb and an LOD of 0.02 ppb in 10:90 acetonitrile/water (v/v). The average coefficients of variation were 4.9 and 7.8% over the range of the standard curve for intra-and interassay precision. Interference caused by 1,6-and 1,8-dinitropyrenes was observed. The effect of different concentrations of solvents on the antibody-analyte interaction was studied.

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“…However, it was shown that interaction of antibody with haptens occurs also in organic solvents [5] and immunochemical applications of this finding quickly followed [6][7][8][9]. Recently, attention is focused on immunosensors operating in the presence of organic solvents as reviewed by Setford et al [10,11].…”

Section: Introductionmentioning

confidence: 96%